Amasya Elmasının SSR, AFLP, E-STS ve RGA Markörleri İle Doymuş Genetik Haritasının Oluşturulması

Abstract

Ülkemizde en önemli yerel elma çeşidi olan Amasya elması, sahip olduğu iyi meyve kalitesiyle önemli bir genetik kaynaktır. Bu çalışmada Amasya elması içerisinden seçilen 'Kaşel-37' ile 'Delbarestivale' çeşidi melezlemesinden elde edilen F1 popülasyonu kullanılarak Amasya elmasının doymuş genetik haritasının elde edilmesi ve sonrasında aroma bileşikleri, şeker ve organik asitler gibi bazı meyve kalite özellikleri ile ilişkili kantitatif özellik lokuslarının belirlenmesi amaçlanmıştır. Çalışma kapsamında 699 SSR, 240 AFLP, 121 E-STS, 24 RGA primer çifti öncelikle ebeveynler ve 6 F1 bitkisinde açılım gösterme bakımından taranmış olup, açılım gösterenler tüm F1 bitkilerinde analiz edilmişlerdir. 'Kaşel-37' çeşidinin genetik haritasında toplam 621 markör yer almış ve toplam harita uzunluğu 1.700,1 cm, ortalama bağlantı grubu uzunluğu ise 100,0 cm olarak hesaplanmıştır. Toplam 17 bağlantı grubu üzerinde, 286 AFLP, 323 SSR, 7 adet E-STS, 2 RGA ve 3 adet farklı özellikler ile ilişkili markörler yer almıştır. 'Delbarestivale' çeşidinin genetik haritasında toplam 557 markör yer almış ve harita uzunluğu 1.826,7 cm, ortalama bağlantı grubu uzunluğu ise 107,5 cm olarak hesaplanmıştır. Toplam 17 bağlantı grubu üzerinde, 254 AFLP, 390 SSR, 7 E-STS, 1 RGA ve 5 adet farklı özellikler ile ilişkili markörler yer almıştır. Sonuç olarak, ‘Kaşel-37’ ve ‘Delbarestivale’ çeşitleri için QTL analizlerinde kullanmak üzere doymuş genetik haritalar oluşturulmuştur.

Keywords

• Elma
• amasya elması
• genetik harita
• mikrosatellit

Construction of a Saturated Genetic Linkage Map of Amasya Apple by SSR, AFLP, E-STS and RGA Markers

Abstract

The most important local cultivar in Turkey is ‘Amasya’ which is a very important genetic source with a good fruit quality. In this study, it was aimed to construct especially genetic map of Amasya cultivar and to determine quantitative trait loci of fruit quality characters such as aroma compounds, sugars etc. by using a F1 population that was created by crossing ‘Kaşel-37’ (female parent; selected from Amasya cultivar) and ‘Delbarestivale’ (male parent) cultivars. 699 SSR, 240 AFLP, 121 E-STS, 24 RGA primer pairs were screened for segregation firstly in the DNAs of parents and 6 F1 plants, then segregated ones were analyzed in all F1 plants. A total of 621 markers were mapped in the genetic map of 'Kaşel-37' cultivar, and the total map was 1.700,1 cm, The average length of linkage groups was calculated 100,0 cm. A total of 17 linkage groups was located with 286 AFLP, 323 SSR, 7 E-STS, 2 RGA and 3 different characters. A total of 557 markers were mapped in the genetic map of 'Delbarestivale' cultivar, and the total map was 1.826.7 cm, The average length of linkage groups was calculated 107.5 cm. A total of 17 linkage groups was located with 254 AFLP, 390 SSR, 7 E-STS, 1 RGA and 5 different characters. In conclusion, saturated genetic maps were established for both ‘Amasya’ and ‘‘Delbarestivale’’ cultivars to use them for QTL analysis.

Keywords

• Apple
• Amasya apple
• genetic map
• microsatellite

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