Biber ve Patlıcanda Katlanmış Haploidi Teknolojisi İle Elde Edilen Saf Hatlarda Moleküler Yöntemlerle Heterotik Grupların Oluşturulması

Abstract

Anter kültürü yöntemiyle elde edilen dihaploid (DH) silindirik tipte 133 patlıcan (Solanum melongena) ve kapya tipinde 128 biber (Capsicum annuum L) genotipinde heterotik grupların oluşturulması amacıyla DNA izolasyonu yapılmıştır. İzolasyonu yapılan DNA’lar genetik benzerlik ve farklılıklarının tespit edilmesi amacıyla SRAP markırlarıyla taranmıştır. Araştırmada 32 adet primer kombinasyonu test edilmiş ve bu primerlerden polimorfik ve skorlanabilir biberde 7, patlıcanda 8 primer kombinasyonu seçilmiştir. Polimorfik primerler bütün patlıcan ve biber genotipleri ile taranmıştır. NTSYS (Numerical Taxonomy Multivariate Analysis System) modülü kullanılarak, Rohlf (2004) metoduna göre elde edilen benzerlik matrisinin UPGMA (Unweighted Pair Group Method Arithmetic Average) gruplandırması ile genetik ilişkinin seviyesi belirlenmiştir. Biberde primerlerin polimorfizm oranı %40 ile %83 arasında değişmiş, ortalama %60 olarak tespit edilmiştir. Biberde 128 genotipin 0.65 ile 1.0 düzeyinde benzer oldukları tespit edilmiştir. Patlıcanda 8 primer kombinasyonu ile toplam 71 bant elde edilmiştir ve bunların 37’si polimorfik bulunmuştur. Tüm primerlerin polimorfizm oranı %52 olarak belirlenmiştir. Genotiplerin benzerlik oranı 0.78 ile 0.99 arasında değişmiştir. Elde edilen dendrogramlarda ve iki boyutlu dağılım grafiğinde tespit edilen bulgular ışığında birbirine en uzak olan hatlar melezlenmek üzere belirlenmiştir.

Keywords

• Solanum melongena
• Capsicum annuum
• DH hat
• markır
• heterotik grup
• hibrit çeşit

Construction of Heterotic Groups With Molecular Methods in Pure Lines Obtained by Doubled Haploid Technology in Pepper and Eggplant

Abstract

DNA isolation was performed for heterotic grouping in DH (doubled haploid) 133 cylindrical type eggplant (Solanum melongena) and 128 pepper (Capsicum annuum L) genotypes obtained by anther culture method. The isolated DNAs were screened with SRAP markers to detect genetic similarities and differences. In the study, 32 primer combinations were tested and 7 primer combinations in polymorphic and score able pepper and 8 primer combinations in eggplant were selected from these primers. Polymorphic primers were analyzed with all eggplant and pepper genotypes. In line with the data obtained, the level of genetic relationship was determined by UPGMA (Unweighted Pair Group Method Arithmetic Average) grouping of the similarity matrix obtained according to the Rohlf (2004) method using the NTSYS (Numerical Taxonomy Multivariate Analysis System) module. The polymorphism ratio of the primers in pepper varied between 40%and 83%, with an average of 60%. It was determined that 128 genotypes were similar between 0.65 and 1.00 in pepper. In eggplant, 71 bands were obtained with 8 primer combinations and 37 of them were found to be polymorphic. Polymorphism ratio of all primers was determined as 52%. The similarity ratio of the genotypes varied between 0.78 and 0.99. In light of the findings determined in the obtained dendrograms and bi-dimensional scatterplots, the lines that are farthest from each other can be hybridized to increase the hybrid power.

Keywords

• Solanum melongena
• Capsicum annuum
• DH lines
• markers
• heterotic groups
• hybrid species

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