Katlanmış Diploid Biberlerde Morfolojik, Sitolojik ve DNA Miktarına Göre Yapılan İnceleme Sonuçlarının Karşılaştırılması

Abstract

Kapya biberlerde, tohumsuz meyve veren çeşitler geliştirmeye yönelik ıslah programlarında kullanılacak tetraploid ebeveynlerin elde edilmesi amacıyla bir dizi çalışma yapılmıştır. Bu kapsamda gerçekleştirilen kolhisin uygulamaları sonucunda gelişen bitkiler/sürgünler üzerindeki çiçeklerin kendilenmesiyle tohumlar elde edilmiştir. Olası tetraploid tohumlar ekilmiş ve bitkiler fidelikte yetiştirilmiştir. Polikarbon serada yerlerine dikilen bitkiler çeşitli morfolojik özellikleri bakımından gözlemlenmiş (yaprak alanı, çiçek çapı, meyve eni / boyu, tohum oluşumu), bu bitkilerin yapraklarında birim alandaki stoma sayıları ve boyutları kayıt altına alınmış, ayrıca genç fide döneminde alınan örneklerde flow sitometri yöntemi ile ploidi seviyesi belirlenmiştir. Böylece morfolojik, sitolojik ve DNA miktarı bakımından yapılan incelemelerin sonuçları bir arada değerlendirilebilmiştir. Üç farklı yöntemden elde edilen sonuçlar karşılaştırılarak aralarındaki ilişkiler araştırılmıştır. Çalışma sonucunda en güvenilir bulguları veren flow sitometri yöntemi esas alınarak, morfolojik ve sitolojik tahminlerin tutarlılığı yorumlanmıştır. Morfolojik ve sitolojik yollarla geniş bir bitki popülasyonu içinde ön seleksiyonun yapılabileceği, seçilen bitkilerin ploidi seviyesinin teyit edilmesi için flow sitometri yoluyla analiz yapılmasının gerekli olduğu sonucuna ulaşılmıştır.

Keywords

• Capsicum annuum
• flow sitometri
• stoma
• ploidi

Comparison of The Examination Results According to Morphological, Cytological and DNA Amount in Doubled Diploid Peppers

Abstract

A series of studies have been carried out to obtain tetraploid parents to be used in breeding programs to develop seedless fruiting varieties in capia peppers. In this context, the seeds obtained by the selfing of flowers on the plants / shoots developed as a result of colchicine applications were sown and the seedlings were grown in the seedling greenhouse. Seedlings planted in the polycarbonate greenhouse were observed in terms of various morphological characteristics (leaf area, flower diameter, fruit width and length, seed formation) and the number of stomas per unit area and size in the leaves of these plants were determined. Also, the ploidy level of plants was determined by flow cytometry method in the samples taken during the young seedling period. Thus, the results of the examinations made in terms of morphological, cytological and DNA amount could be evaluated together. At the end of the study, the consistency of morphological and cytological predictions was interpreted based on the flow cytometry method, which gave the most reliable result. It has been concluded that preselection can be made in a large plant population by morphological and cytological means, but analysis by flow cytometry is necessary to confirm the ploidy level of the selected plants.

Keywords

• Capsicum annuum
• flow cytometry
• stoma
• ploidy

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