MicroRNA’ların Domates Bitkisinde Abiyotik Stres Faktörlerine Karşı Toleranslığa Etkisi

Abstract

21-24 nt uzunluğunda kodlanmayan RNA’lar arasında bulunan mikro RNA’lar çeşitli gen regülasyonların da aktif bir şekilde rol almaktadırlar. Bu husus özellikle stres anlarında meydana gelmekte olup bitki tolerantlığında/dayanımında olumlu veya olumsuz olarak rol oynamaktadırlar. Bu sebeple bitki ıslahında kullanımı önemli bir potansiyele sahiptir. Özellikle biyoteknolojinin de gelişmesiyle mikro RNA aracılığı ile genomda çeşitli düzenlemeler yapılabilmekte olup CRISPR, Prime editing gibi teknolojiler ile beraber direkt olarak MIR geneleri hedeflenip düzenleme de yapılabilmektedir. Bu sayede model bitkilerin yanında ekonomik değeri yüksek bitkilerin ıslahında da önemli bir araç olarak da kullanılabilmektedir. Dünya üzerinde en çok üretilen sebzelerin başında gelen domates bu türlerden birisidir. Bu makalede mikro RNA moleküllerinin abiyotik stres faktörleri özelinde domates bitkisinde kullanım olanakları tartışılmıştır.

Keywords

• Domates
• abiyotik stres faktörleri
• mikro RNA
• kısa kodlanmayan RNA’lar

The Effects of MicroRNAs on Tolerance of Abiotic Stress Factors in Tomato Plant

Abstract

Micro RNAs are among the 21-24 nt long non-coding RNAs, playing an active role in various gene regulation. This happens especially when plants are exposed to stress factors, leading to positive or negative regulations. Therefore, miRNAs have an important potential. Nowadays, micro-RNA based gene regulation can be manipulated in the genome. Furthermore, through the today’s technological tools such as CRISPR and prime editing, Micro RNA coding genes (MIR) can be up or down regulated. Thus, in addition to model crops, economically important crops can also be improved easily thanks to these tools. Tomato, one of the most produced vegetables is among these plants. In this article, the possibilities of using micro-RNA molecules in tomato plants will be discussed in terms of abiotic stress factors.

Keywords

• Tomato
• abiotic stress factors
• micro RNAs
• small non-coding RNAs

References

1. Banerjee, S., Sirohi, A., Ansari, A.A., Gill, S.S. 2017. Role of small RNAs in abiotic stress responses in plants. Plant Gene, 11:180-189.
2. Bao, N., Lye, K.W., Barton, M.K., 2004. MicroRNA binding sites in Arabidopsis class III HD-ZIP mRNAs are required for methylation of the template chromosome. Dev. Cell 7:653-662. (https://doi.org/10.1016/j.devcel.2004.10.003).
3. Basso, M.F., Ferreira, P.C.G., Kobayashi, A.K., Harmon, F. G., Nepomuceno, A. L., Molinari, H. B.C., Grossi‐de‐Sa, M.F. 2019. Micro RNAs and new biotechnological tools for its modulation and improving stress tolerance in plants. Plant Biotechnology Journal, 17(8):1482-1500.
4. Cao, D., Wang, J., Ju, Z., Liu, Q., Li, S., Tian, H., Fu, D., Zhu, H., Luo, Y., Zhu, B. 2016. Regulations on growth and development in tomato cotyledon, flower and fruit via destruction of miR396 with short tandem target mimic. Plant Science:An International Journal of Experimental Plant Biology 247:1-12 (https://doi.org/10.1016/j. plantsci.2016.02.012).
5. Cardoso, T.C.D.S., Alves, T.C., Caneschi, C.M., Santana, D.D.R.G., Fernandes-Brum, C.N., Reis, G.L.D., ... de Souza Gomes, M. 2018. New insights into tomato microRNAs. Scientific Reports 8(1):1-22.
6. Djami-Tchatchou, A.T., Sanan-Mishra, N., Ntushelo, K., Dubery, I.A., 2017. Functional roles of microRNAs in Agronomically important plants-potential as targets for crop improvement and protection. Front. Plant Sci. 8 (https://doi.org/ 10.3389/ fpls.2017.00378).
7. Fracasso, A., Vallino, M., Staropoli, A., Vinale, F., Amaducci, S., Carra, A. 2021. Increased water use efficiency in miR396-downregulated tomato plants. Plant Science 303:110729.
8. Kar, M.M., Raichaudhuri, A. 2021. Role of microRNAs in mediating biotic and abiotic stress in plants. Plant Gene 26:100277.

9. Khraiwesh, B., Arif, M.A., Seumel, G.I., Ossowski, S., Weigel, D., Reski, R., Frank, W., 2010. Transcriptional control of gene expression by microRNAs. Cell 140:111-122 (https://doi.org/ 10.1016/j.cell.2009.12.023).
10. Kim, W., Benhamed, M., Servet, C., Latrasse, D., Zhang, W., Delarue, M., Zhou, D.X., 2009. Histone acetyltransferase GCN5 interferes with the miRNA pathway in Arabidopsis. Cell Res. 19:899-909 (https://doi.org/10.1038/cr.2009.59).
11. Mandal, K., Boro, P., Chattopadhyay, S. 2021. Micro-RNA based gene regulation:A potential way for crop improvements. Plant Gene 27:100312.
12. Ouyang, S., Park, G., Atamian, H.S., Han, C.S., Stajich, J.E., Kaloshian, I., Borkovich, K.A. 2014. MicroRNAs suppress NB domain genes in tomato that confer resistance to Fusarium oxysporum. PLoS Pathogens 10(10):e1004464.
13. Rao, S., Balyan, S., Jha, S., Mathur, S. 2020. Novel insights into expansion and functional diversification of MIR169 family in tomato. Planta 251(2):1-17.
14. Rodriguez-Enriquez, J., Dickinson, H.G., Grant-Downton, R.T., 2011. MicroRNA misregulation:an overlooked factor generating soma clonal variation? Trends Plant Sci. 16:242-248 (https:// doi.org/10.1016/j.tplants.2011.03.002).
15. Wang, W., Luan, Y. 2015. The advance of tomato disease-related microRNAs. Plant Cell Reports, 34(7):1089-1097.
16. Wilkinson, S., Kudoyarova, G.R., Veselov, D.S., Arkhipova, T.N., Davies, W.J. 2012. Plant hormone interactions:innovative targets for crop breeding and management. Journal of Experimental Botany 63(9):3499-3509.
17. Wu, L., Zhou, H., Zhang, Q., Zhang, J., Ni, F., Liu, C., Qi, Y., 2010. DNA methylation mediated by a microRNA pathway. Mol. Cell 38:465-475. (https://doi.org/10.1016/j.molcel.2010.03.008).
18. Yang, G., Li, Y., Wu, B., Zhang, K., Gao, L., Zheng, C., 2019. MicroRNAs transcriptionally regulate promoter activity in Arabidopsis thaliana. J. Integr. Plant Biol. 61:1128-1133. (https://doi. org/10.1111/jipb.12775).
19. Yang, X., Luan, Y.S. 2021. Preliminary study of Sly-miR399 in tomato resistance to late blight. China Biotechnology 41(11):23-31.
20. Zhou, M., Li, D., Li, Z., Hu, Q., Yang, C., Zhu, L., Luo, H., 2013. Constitutive expression of a miR319 gene alters plant development and enhances salt and drought tolerance in transgenic creeping bentgrass. Plant Physiol. 161(3):1375-1391 (https://doi.org/ 10.1104/pp.112.208702).