The using of biotechnology in cultivar breeding of Cucurbita vegeteble species

Abstract

Compared to conventional breeding methods, biotechnology is approved a more effective crop improvement technique, requiring only a short time to develop a new variety. Thus, biotechnogical breeding methods are distinguished in current breeding efforts. In view of this concept, this presented study aimed to provide an overview of biotechnology–based approaches in Cucurbita breeding programs. In this way, some biotechnological breeding methods (dihaploidization, interspecific hybridization, regeneration, protoplast culture, recombinant DNA technology, molecular techniques) and their applications were discussed. The results of study could provide a different perspective to Cucurbita breeders for the improvement of new varieties with desirable agronomic and economic traits.

Keywords

• Cucurbita,breeding,cultivar,biotechnology

Kabak türlerinin çeşit ıslahında biyoteknolojinin kullanımı

Abstract

Yeni bir çeşidin geliştirilmesinde biyoteknoloji, konvansiyonel ıslah metotlarıyla karşılaştırıldığında etkili bir ürün geliştirme tekniği olarak kabul görmektedir. Bu sebeple günümüz ıslah çalışmalarında biyoteknolojik ıslah metotları daha fazla ön plana çıkmaktadır. Sunulan bu derleme çalışmasında, Cucurbita türlerinin çeşit ıslah programlarında bazı biyoteknolojik ıslah metotları (dihaploidizasyon, türler arası melezlemeler ve embriyo kültürü, rejenerasyon, protoplast kültürü, rekombinant DNA teknolojisi, moleküler teknikler) ve bunların uygulamaları tartışılmıştır. Çalışmanın sonuçları, Cucurbita türlerinde çalışan ıslahçılara istenilen agronomik ve ekonomik özelliklere sahip yeni çeşitlerin geliştirilmesinde farklı bir bakış açısı sunabilecektir.

Keywords

• Cucurbita,ılsah,çeşit,biyoteknoloji

References

1. Abrie, A. L. and J. Van Staden, 2001. Micropropagation of the Endangered Aloe polyphylla. Plant Growth Regulation 33(1): 19–23.
2. Aggour, A. R., L. A. Badr and M. M. Ashry, 1999. Biotechnological Studies on Interspecific Crosses among Some Cucurbita Species. First International Conference in Egypt, on Plant Tissue Culture and its Application 255–275.
3. Ananthakrishnan, G., X. Xia, C. Elman, S. Singer, H. S. Paris, A. Gal–on and V. Gaba, 2003. Shoot Production in Squash (Cucurbita pepo) by in vitro Organogenesis. Cell Biology and Morphogenesis.
4. Andres, T. C. and R. W. Robinson, 2002. Cucurbita Ecuadorensis, an Ancient Semi–Domesticate With Multiple Disease Resistance and Tolerance to Some Adverse Growing Conditions. In: D. N. Maynard (ed.). Cucurbitaceae 2002. ASHS Press, Alexandria, Va. p. 95–99.
5. Baktemur, G., N. K. Yücel, H. Taşkın, S. Çömlekçioğlu ve S. Büyükalaca, 2014. Effects of Different Genotypes and Gamma Ray Doses on Haploidization Using Irradiated Pollen Technique in Squash. Turkis Journal of Biology 38:318–327.
6. Balkaya, A., E. S. Kurtar, R. Yanmaz ve M. Ozbakır, 2005. Karadeniz Bölgesinde Kışlık Kabak Türlerinde (Kestane Kabağı, Cucurbita maxima Duchesne ve Balkabağı, Cucurbita moschata Duchesne) Gen Kaynaklarının Toplanması, Karakterizasyonu ve Değerlendirilmesi. TÜBİTAK 104O144 nolu Proje Sonuç Raporu 160s.
7. Baranek, M., G. Stift, J. Vollmann and T. Lelly, 2000. Genetic Diversity within and between the Species Cucurbita pepo, C. moschata and C. maxima as Revealed by Rapd Markers. Cucurbit Genetics Cooperative Report 23:73–77.
8. Berber, M., 2009. Kabuksuz Çekirdek Kabaklarında (Cucurbita pepo L. var. styriaca) Işınlanmış Polenle Tozlama Yöntemiyle Haploid Üretimi (Yüksek Lisans Tezi). Çukurova Üniversitesi Fen Bilimleri Enstitüsü Biyoteknoloji Anabilim Dalı, 62 s.

9. Blanca, J., J. Canizares, C. Roig, P. Ziarsolo, F. Nuez and B. Pico, 2011. Transcriptome Characterization and High Throughput SSRs and SNPs Discovery in Cucurbita pepo (Cucurbitaceae). BMC Genomics 12:1–15.
10. Bordas, M., L. Gonzáles–Candelas, M. Dabauza, D. Ramón and V. Moreno, 1998. Somatic Hybridization between an Albino Cucumis melo L. Mutant and Cucumis myriocarpus Naud. Plant Science 132:179–190.
11. Chambonnet, D. and R. Dumas De Vaulx, 1985. Obtention of Embryos and Plants from in vitro Culture of Unfertilized Ovules of Cucurbita Pepo. Cucurbit Genetics Cooperative Report 8:66.
12. Çağlar, G. ve S. Bağcı, 2004. Bazı Cucumis Türleri Arasındaki Melezlemelerde Embriyo Kurtarma Yoluyla in vitro Hibrit Bitki Regenerasyonu. Akdeniz Üniversitesi Ziraat Fakültesi Dergisi 17(2):175–182.
13. Dumas De Vaulx, R. and D. Chambonnet, 1986. Obtention of Embryos and Plants from in vitro Culture of Unfertilized Ovules of Cucurbita pepo. In: Genetic Manipulation in Plant Breeding. W. Horn, C. J. Jensen, W. Odenbach, O. Schieder (eds), Proc. International Symposium Eucarpia, 8–12 Sept. 1985, Berlin. 295–297.
14. Esteras, C., P. Gomez and A. J. Monforte, 2012. High–Throughput SNP Genotyping in Cucurbita pepo for Map Construction and Quantitative Trait Loci Mapping. BMC Genomics 13:1–21.
15. Ferriol, M., B. Pico and F. Nuez, 2003. Genetic Diversity of a Germplasm Collection of Cucurbita pepo using SRAP and AFLP Markers. Theoretical and Applied Genetics 107:271–282.
16. Ferriol, M., B. Pico, P. Fernandez and F. Nuez, 2004a. Molecular Diversity of a Germplasm Collection of Squash (Cucurbita moschata) Determined by SRAP and FLP Markers. Crop Science 44:653–664.
17. Ferriol, M., B. Pico and F. Nuez, 2004b. Morphological and Molecular Diversity of a Collection of Cucurbita maxima Landraces. Journal of the American Society for Horticultural Science 129(1):60–69.
18. Gajdova J., A. Lebeda and B. Navrátilová, 2004. Protoplast Cultures of Cucumis and Cucurbita spp. In: Lebeda A., Paris H. S. (eds), Progress in Cucurbit Genetics and Breeding Research. Proceedings of Cucurbitaceae 2004, the 8th Eucarpia Meeting on Cucurbit Genetics and Breeding. Palacký University in Olomouc, Olomouc: 441–454.
19. Gajdova, J., B. Navrátilová, J. Smolná and A. Lebeda, 2007. Effect of Genotype, Source of Protoplasts and Media Composition on Cucumis and Cucurbita Protoplast Isolation and Regeneration. Acta Horticulturae 731:89–94.
20. Gémes Juhász, A., G. Venczel and P. Balogh, 1997. Haploid Plant Induction in Zucchini (Cucurbita pepo L. convar. giromontiina Duch) and in Cucumber (Cucumis sativus L.) Lines Through in vitro Gynogenesis. Acta Horticulturae 447:623–625.
21. Gemesne, J. A. and G. Venczel, 1996. in vitro Gynogenesis Induction in Zucchini (Cucurbita pepo L. convar. giromontiina Duch) Lines. Proceedeings of the VI. Eucarpia Meeting on Cucurbit Genetics and Breeding (1996) pp: 200–201.
22. Gong, L., G. Stift, R. Kofler, M. Pachner and T. Lelley, 2008a. Microsatellites for the Genus Cucurbita and an SSR–based Genetic Linkage Map of Cucurbita pepo L. Theoretical and Applied Genetics 117:37–48.
23. Gong, L., M. Pachner, K. Kalai and T. Lelley, 2008b. SSR–based Genetic Linkage Map of Cucurbita moschata and its Synteny with Cucurbita pepo. Genome 51:878–887.
24. Jarl, C. I., G. S. Bokelmann and J. M. De Haas, 1995. Protoplast Regeneration and Fusion in Cucumis: melon × cucumber. Plant Cell Tissue and Organ Culture 43:259–265.
25. Kathiravan, K., G. Vengedesan, S. Singer, B. Steinitz, H. S. Paris and V. Gaba, 2006. Adventitious Regeneration in vitro Occurs Across a wide Spectrum of Squash (Cucurbita pepo) genotypes. Plant Cell Tissue and Organ Culture 85:285–295.
26. Kintzios, S., E. Sereti, P. Bluchos, J. B. Drossopoulos, C. K. Kitsaki and A. Liopa–Tsakalidis, 2002. Growth Regulator Pretreatment Improves Somatic Embryogenesis from Leaves of Squash (Cucurbita pepo L.) and Melon (Cucumis melo L.). Plant Cell Reports 21:1–8.
27. Kiss–Baba, E., S. Panczel, K. Simonyi and G. D. Bisztray, 2010. Investigations on the Regeneration Ability of Squash Cultivars. Acta Agronomica Hungarica 58(2):159–166.
28. Klas, F. E., M. Fuchs and D. Gonsalves, 2011. Fruit Yield of Virus–Resistant Transgenic Summer Squash in Simulated Commercial Plantings under Conditions of High Disease Pressure. Journal of Horticulture and Forestry 3(2):46–52.
29. Kurtar, E. S., N. Sarı ve K. Abak, 2000. Kabakta Bazı Cucurbita Türleri ile Tozlamanın Haploid Embriyo Uyartımına Etkileri. OMÜ Ziraat Fakültesi Dergisi 15(2):43–47.
30. Kurtar, E. S., N. Sarı and K. Abak, 2002. Obtention of Haploid Embryos and Plants through Irradiated Pollen Technique in Squash (Cucurbita pepo L.). Euphytica 127:335–344.
31. Kurtar, E. S., A. Balkaya, M. Özbakır and T. Ofluoğlu, 2009. Induction of Haploid Embryo and Plant Regeneration via Irradiated Pollen Technique in Pumpkin (Cucurbita moschata Duchesne ex. Poir). African Journal of Biotechnology 8(21):5944–5951.
32. Kurtar, E. S. and A. Balkaya, 2010. Production of in vitro haploid Plants from in Situ Induced Haploid Embryos in Winter Squash (Cucurbita maxima Duchesne ex Lam.) Via Irradiated Pollen. Plant Cell Tissue and Organ Culture 102(3):267–277.
33. Kurtar, E. S., A. Balkaya and D. Kandemir, 2016. Evaluation of Haploidization Efficiency in Winter Squash (Cucurbita maxima Duch.) and Pumpkin (Cucurbita moschata Duch.) Through Anther Culture. Plant Cell Tissue and Organ Culture 127:497–511.
34. Kwack, S. N. and K. Fujieda, 1988. Somatic Embryogenesis in Cultured Unfertilized Ovules of Cucurbita moschata. Journal of the Japanese Society for Horticultural Science 57:34–42.
35. Lee, Y. K. and W. I. Chung, 2002. Plant Regeneration via Organogenesis in the Korean and Japanese Winter Squash (Cucurbita Maxima). Proc. 2nd IS on Cucurbits. Acta Horticulturae 588.
36. Lee, Y. K., W. I. Chung and H. Ezura, 2003. Efficient Plant Regeneration via Organogenesis in Winter Squash (Cucurbita maxima Duch.). Plant Science 164:41–418.
37. Metwally, E. I., S. A. Haroun and G. A. El–Fadly, 1996. Interspecific Cross between Cucurbita pepo L. and Cucurbita martinezii Through in vitro Embryo Culture, Euphytica 90:1–7.
38. Metwally, E., S. A. Moustafa, B. I. El–Sawy, S. A. Harun and T. A. Shalaby, 1998a. Production of Haploid Plants from in vitro Culture of Unpollinated Ovules of Cucurbita pepo. Plant Cell Tissue and Organ Culture 52(3):117–121.
39. Metwally, E., S. A. Moustafa, B. I. El–Sawy and T. A. Shalaby, 1998b. Haploid Plantlets Derived by Anther Culture of Cucurbita pepo. Plant Cell Tissue and Organ Culture 52(3):171–176.
40. Mohammed, M. F. and E. F. S. Refaei, 2004. Enhanced Haploids Regeneration in Anther Culture of Summer Squash (Cucurbita pepo L.). Cucurbit Genetics Cooperative Report 27:57–60.
41. Mookhan, M., 2015. Direct Organogenesis from Cotyledonary Node Explants of Cucurbita pepo (L.) An Important Zucchini Type Vegetable Crop. American Journal of Plant Science 6:157–162
42. Murashige, T. and F. Skoog, 1962. A revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Plant Physiol 15:473–497.
43. Oliveira, A. C. B., W. R. Maluf, J. E. B. P. Pinto and S. M. Azevedo, 2003. Resistance to Papaya Ringspot Virus in Summer Squash Cucurbita pepo L. Introgressed from an Interspecific C. pepo × C. moschata cross. Euphytica 132:211–215.
44. Pal, S. P., I. Alam, M. Anisuzzaman and K. K. Sarker, 2007. Indirect Organogenesis in Summer Squash (Cucurbita pepo L.). Turkish Journal of Agriculture 31:63–70.
45. Paris, H. S., N. Yonash, V. Portnoy, N. Mozes–Daube, G. Tzuri and N. Katzir, 2003. Assessment of Genetic Relationships in Four Cucurbita pepo (Cucurbitaceae) Using DNA markers. Theoretical Applied Genetics 106:971–978.
46. Rakha, M. T., E. I. Metwally, S. A. Moustafa, A. A. Etman and Y. H. Dewir, 2012. Production of Cucurbita Interspecific Hybrids through Cross Pollination and Embryo Rescue Technique. World Applied Sciences Journal 20(10):1366–1370.
47. Robinson, R. W., 1987. Inheritance of Fruit Skin Color in Cucurbita Moschata. Cucurbit Genetics Cooperative Report 10:84.
48. Sarover, S., H. Y. OH, N. I. Hyung, B. W. Min, C. H. Harn, S. K. Yang, S. H. OK and J. S. Shin, 2003. In vitro Micropropagation of a Cucurbita Interspecific Hybrid Cultivar–a Root Stock Plant. Plant Cell Tissue and Organ Culture 75:179–182.
49. Shail, J. W. and R. W. Robinson, 1987. Anther and Ovule Culture of Cucurbita. Cucurbit Genetics Cooperative Report 10:92.
50. Shalaby, T. A., 2007. Factors Affecting Haploid Induction Through in vitro Gynogenesis in Summer Squash (Cucurbita pepo L.). Science Horticultural 115(1):1–6.
51. Sisko, M., A. Ivancic and B. Bohanec, 2003. Genome Size Analysis in the Genus Cucurbita and its use for Determination of Interspecific Hybrids Obtained Using the Embryo–Rescue Technique. Plant Science 165:663–669.
52. Stipp, L. C. L., B. A. Cristina, M. Hara, B. M. J. Mendes, 2012. In vitro Rganogenesis of Zucchini Squash cv. Caserta. Horticultura Brasileira 30:274–278.
53. Tricoli, D., K. J. Carney, P. F. Russell, J. R. McMaster, D. W. Groff, K. C. Hadden, P. T. Himmel, J. P. Hubbard, M. L. Boeshore and H. D. Quemada, 1995. Field Evaluation of Transgenic Squash Containing Single or Multiple Virus Coat Protein Gene Constructs for Resistance to Cucumber Mosaic Virus, Watermelon Mosaic Virus 2 and Zucchini Yellow Mosaic Virus. Nature Biotechnology 13:1458–1465.
54. Tuncer, B., 2013. Callus Proliferation and Shoot Regeneration from Different Explant Types in Ornamental Gourd (Cucurbita pepo var. ovifera). Yüzüncü Yıl University Journal Agricultural Science 23(2):164–171.
55. Urbanek, A., B. Zechmann and M. Muller, 2004. Plant Regeneration via Somatic Embryogenesis in Styrian Pumpkin: Cytological and Biochemical Investigations. Plant Cell Tissue and Organ Culture 79:329–340.
56. Wu, T., S. Luo and R. Wang. 2014. The First Illumina-Based De Novo Transcriptome Sequencing and Analysis of Pumpkin (Cucurbita moschata Duch.) and SSR Marker Development. Mol Breeding 34:1437–1447.
57. Wyatt, L. E., S. R. Strickler, L. A. Mueller and M. Mazourek, 2015. An Acorn Squash (Cucurbita pepo ssp. ovifera) Fruit and Seed Transcriptome as a Resource for the Study of Fruit Traits in Cucurbita. Horticulture Research, doi: 10.1038/hortres.2014.70.
58. Xie, B., X. F. Wang and Z. C. Fan, 2006. Improved Conditions of in vitro Culture of Unpollinated Ovules and Production of Embryonary Sac Plants in Summer Squash (Cucurbita pepo L.). Scientia Agricultura Sinica 39(1):132–138.
59. Yarımoğlu, M., 2004. Bazı Yazlık Kabak Çeşitlerinde (Cucurbita pepo L.) in vitro Bitki Regenerasyonu (Yüksek Lisans Tezi). Çukurova Üniversitesi Fen Bilimleri Enstitüsü, 35s.
60. Yılmaz, Ö., 2005. Yazlık Kabakta (Cucurbita pepo L.) Ovaryum Kültürü Yoluyla Haploid Bitki Elde Edilmesi (Yüksek Lisans Tezi). Kahramanmaraş Sütçü İmam Üniversitesi Fen Bilimleri Enstitüsü, 66s.
61. Youn, S. J. and H. D. Chung, 1998. Genetic Relationships among the Local Varieties of the Korean Native Squashes (Cucurbita moschata) Using RAPD Technique. Journal of the Korean Society for Horticultural Science 39:517–521.
62. Zhang, Y., J. Zhou, T. Wu and J. Cao, 2008. Shoot Regeneration and the Relationship between Organogenic Capacity and Endogenous Hormonal Contents in Pumpkin. Plant Cell Tissue and Organ Culture 93:323–331.
63. Zraidi, A., G. Stift, M. Pachner, A. Shojaeiyan, L. Gong and T. A. Lelley, 2007. Consensus Map for Cucurbita pepo. Mol Breeding 20:375–388.