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Amasya Elmasının SSR, AFLP, E-STS ve RGA Markörleri İle Doymuş Genetik Haritasının Oluşturulması

Year 2016, Volume: 45 Issue: (Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi, 223 - 229, 31.03.2016

Murat Güney , Nergiz Çoban Mortaza Khodaeiaminjan Hayat Topçu , Ebru Kafkas , Şerif Özongun , Ayşe Nilgün Atay , Kadir Uğurtan Yılmaz , Salih Kafkas

Abstract

Ülkemizde en önemli yerel elma çeşidi olan Amasya elması, sahip olduğu iyi meyve kalitesiyle önemli bir genetik kaynaktır. Bu çalışmada Amasya elması içerisinden seçilen 'Kaşel-37' ile 'Delbarestivale' çeşidi melezlemesinden elde edilen F1 popülasyonu kullanılarak Amasya elmasının doymuş genetik haritasının elde edilmesi ve sonrasında aroma bileşikleri, şeker ve organik asitler gibi bazı meyve kalite özellikleri ile ilişkili kantitatif özellik lokuslarının belirlenmesi amaçlanmıştır. Çalışma kapsamında 699 SSR, 240 AFLP, 121 E-STS, 24 RGA primer çifti öncelikle ebeveynler ve 6 F1 bitkisinde açılım gösterme bakımından taranmış olup, açılım gösterenler tüm F1 bitkilerinde analiz edilmişlerdir. 'Kaşel-37' çeşidinin genetik haritasında toplam 621 markör yer almış ve toplam harita uzunluğu 1.700,1 cm, ortalama bağlantı grubu uzunluğu ise 100,0 cm olarak hesaplanmıştır. Toplam 17 bağlantı grubu üzerinde, 286 AFLP, 323 SSR, 7 adet E-STS, 2 RGA ve 3 adet farklı özellikler ile ilişkili markörler yer almıştır. 'Delbarestivale' çeşidinin genetik haritasında toplam 557 markör yer almış ve harita uzunluğu 1.826,7 cm, ortalama bağlantı grubu uzunluğu ise 107,5 cm olarak hesaplanmıştır. Toplam 17 bağlantı grubu üzerinde, 254 AFLP, 390 SSR, 7 E-STS, 1 RGA ve 5 adet farklı özellikler ile ilişkili markörler yer almıştır. Sonuç olarak, ‘Kaşel-37’ ve ‘Delbarestivale’ çeşitleri için QTL analizlerinde kullanmak üzere doymuş genetik haritalar oluşturulmuştur.

Keywords

Elma amasya elması genetik harita mikrosatellit

References

  • Conner, P.J., Brown, S.K., Weeden, N.F., 1997. Randomly amplified polymorphic DNA-based genetic linkage maps of three apple cultivars. J. Amer. Soc. for Hort. Sci., 122:350-359.
  • Doyle, J.J., Doyle, J.L., 1987. A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19:11-15.
  • FAOSTAT, 2015. Agriculture data [online]. http://faostat3.fao.org/browse/q/qc/e.
  • Fernandez, F.F., Harvey, N. G., James, C.M., 2006. Isolation and characterization of polymorphic microsatellite markers from European pear (Pyrus communis L.). Molecular Ecology Notes 6:1039–1041.
  • Grattapaglia, D., Sederoff, R., 1994. Genetic linkage maps of eucalyptus grandis and eucalyptus urophylla using a pseudo-testcross:mapping strategy and RAPD markers. Genetics 137:1121-1137.
  • Guilford, P., Prakash, S., Zhu, J.M., Rikkerink, E., Gardiner, S., Bassett, H., Forster, R., 1997. Microsatellites in Malus x domestica (apple):abundance, polymorphism and cultivar identification. Theoretical and Applied Genetics, 97:249-254.
  • Gianfranceschi, L., Seglias, N., Tarchini, R., Komjanc, M., Gessler, C., 1998. Simple sequence repeats for the genetic analysis of apple. Theoretical and Applied Genetics 96:1069-1076.
  • Han, Y., Chagne, D., Gasic, K., Rikherink, E.H.A., Beever, J.E., Gardiner, S.E., Korban, S.S., 2009. BAC-end sequence-based SNPs and Bin mapping for rapid integration of physical and genetic maps in apple. Genomics 93:282-288.
  • Han, Y., Zheng, D., Vimolmangkang, S., Khan, M.A., Beever, J.E., Korban, S.S., 2011. Integration of physical and genetic maps in
  • apple confirms whole-genome and segmental duplications in the apple genome. J. of Experimental Botany, 1-14.
  • Hemmat, M., Weeden, N.F. and Brown, S.K., 2003. Mapping and evaluation of Malus x domestica microsatellites in apple and pear. J. Amer. Soc. Hort. Sci., 128 (4):515-520.
  • Hokanson, S.C., Szewc-McFadden, A.K., Lamboy, W.F., McFerson, J.R., 1998. Microsatellite (SSR) markers reveal genetic identities, genetic diversity and relationships in a Malus x domestica borkh. Core subset collection. Theoretical and Applied Genetics 97:671683.
  • Hemmat, M., Weeden, N.F., Manganaris, A.G., Lawson, D.M., 1994. Molecular marker linkage map for apple. J. Heredity 85:4-11.
  • Inoue, E., Matsukı, Y., Anzai, H., Evans, K., 2007. Isolation and characterization of microsatellite markers in Japanese pear Molecular Ecology Notes, 7:445–447.
  • Kafkas, E., Atasay, A., Sabır, F., Akgül, H., Uçgun, K., 2010. Effects of different irrigation intervals and fertilizer applications on certain biochemical contents of ‘Breaburn’ apple cultivar. African J. of Biotechnology, 8(10):2138-2142.
  • Kafkas, S., 2006. Phylogenetic analysis of the genus Pistacia by AFLP markers. Plant Systematics and Evolution 262:113-124.
  • Kafkas, S., Özgen, M., Doğan, Y., Özcan, B., Ercişli, S., Serçe, S., 2008. Molecular characterization of mulberry accessions in Turkey by AFLP markers. J. Amer. Soc. for Hort. Sci., 13:593597.
  • Khan, M.A., Han, Y., Zhao, Y.F., Troggio, M., Korban, S.S., 2012, A Multi-population consensus genetic map reveals inconsistent marker order among maps likely attributed to structural variation in the apple genome. Plos One 7:11.
  • Kosambi, D.D., 1944. The estimation of map distances from recombination values. Annals. Eugenics 12:172-175.
  • Liebhard, R., Gianfranceschi, L., Koller, B., Ryder, C.D., Tarchini, R., Weg, Van De E., Gessler, C., 2002. Development and characterisation of 140 new microsatellites in apple. Molecular Breeding 10:217–241.
  • Liebhard, R., Koller, B., Potacchi, A., Kellerhals, M., Pfammatter, W., Jermini, M., Gessler, C., 2003. Mapping quantitative field resistance against apple scap in a ‘Fiesta’ x ‘Discovery’ progeny. Phytopathology 93:493-501.
  • Maliepaard, C., Alston, F.H., Arkel, van G., Brown, L.M., Chevreau, E., Dunemann, F., Evans, K.M., Gardiner, S., Guilford, P., Heusden, van A.W., Janse, J., Laurens, F., Lynn, J.R., Manganaris, A.G., Nijs, den A.P.M., Periam, N., Rikkerink, E., Roche, P., Ryder, C., Sansavini, S., Schmidt, H., Tartarini, S., Verhaegh, J.J., Ginkel, van- M.V., King, G.J., 1998. Aligning male and female linkage maps of apple (Malus pumila Mill.) using multiallelic markers. Theoretical and Applied Genetics 97 (1-2):60-73.
  • Naik, S., Hampson, C., Gasic, K., Bakkeren, G., Korban S.S., 2006. Development and linkage mapping of E-STS and RGA markers for functional gene homologues in apple. Genome 49:959–968.
  • Oddou-Muratorio, S., Aligon, C., Decroocq, S., Plomion, C., Lamant, T., Mush-Demesure, B., 2001. Microsatellite primers for Sorbus torminalis and related species. Molecular Ecology Notes 1:297-299.
  • Özçağıran, R., Ünal, A., Özeker, E., İsfendiyaroğlu, M., 2004. Ilıman Iklim Meyve TürleriYumuşak Çekirdekli Meyveler. Ege Üniv. Ziraat Fak. Yayınları 556, 2:7-9.
  • Silfverberg-Dilworth, E., Matasci, C.L., Van de Weg, W.E., Van Kaauwen, M.P.W., Walser, M., Kodde, L.P., Soglio, V., Gianfranceschi, L., Durel, C.E., Costa, F., Yamamoto, T., Koller, B., Gessler, C., Patocchi, A., 2006. Microsatellite markers spanning the apple (Malus x domestica Borkh.) genome. Tree Genetics & Genomes 2 (4):202-224.
  • Şahinoğlu, A.R., 2011. Bazı elma çeşitlerinde soğuklama gereksinimlerinin saptanması ve subtropik koşullara uygunluğunun incelenmesi. Yüksek Lisans Tezi. Çukurova Üniversitesi Fen Bilimleri Enstitüsü, 55s.
  • Testolin, R., Marrazzo, T., Cipriani, G., Quarta, R., Verde, I., Dettori, M.T., Pancaldi, M., Sansavini, S., 2000. Microsatellite DNA in peach (Prunus persica L. Batsch) and its use in fingerprinting and testing the genetic origin of cultivars. ProQuest Health and Medical Complete 43 (3):512.
  • Van Ooijen, J.W., 2011. JoinMap 4.1, Software for the calculation of genetic linkage maps in experimental populations. Kiyazma B.V., Wageningen, Netherlands.
  • Voorrips, R.E., 2002. Map Chart: software for the graphical presentation of linkage maps and QTLs. The Journal of Heredity 93:77-78.
  • Vos, P., Hogers, L., Bleeker, M., Van De Lee, T., Hornes, m., Frijters, A., Pot, J., Peleman, J., Kuiper, M., Zabeau, M., 1995. AFLP:A new technique for DNA fingerprinting. Nucleic Acids Research 23:4407-4414.
  • Wang, A., Aldwinckle, H., Forsline, P., Main, D., Matsuta, N., 2006b. Simple sequence repeats Fazio, G., Brown, S., Xu, K., 2012. EST for genetic analysis in pear. Euphytica. 124:contig-based SSR linkage maps for Malus x 129-137.
  • domestica cv Royal Gala and an apple scab Zhang, Q., Ma, B., Li, H., Chang, Y., Han, Y., Li, J., resistant accession of M. sieversii, the Wei, G., Zhao, S., Khan, M. A., Zhou, Y., Gu, progenitor species of domestic apple. C., Zhang, X., Han, Z., Korban, S. S., Li, S., Han, Y. ,2012. Identification, characterization, Molecular Breeding 29:379-397.
  • Yamamoto, T., Kimura, T., Shoda, M., Ban, Y., and utilization of genome-wide simple Hayashi, T., Matsuta, N., 2002a. Development sequence repeats to identify a QTL for acidity of microsatellite markers in the Japanese pear in apple. BMC Genomics 13:537.
  • Yamamoto, T., Kimura T., Sawamura, Y., Manabe, T., Kotobuki, K., Hayashi, T., Ban, Y., Mole. Eco.Notes 2:14-16.

Construction of a Saturated Genetic Linkage Map of Amasya Apple by SSR, AFLP, E-STS and RGA Markers

Year 2016, Volume: 45 Issue: (Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi, 223 - 229, 31.03.2016

Murat Güney , Nergiz Çoban Mortaza Khodaeiaminjan Hayat Topçu , Ebru Kafkas , Şerif Özongun , Ayşe Nilgün Atay , Kadir Uğurtan Yılmaz , Salih Kafkas

Abstract

The most important local cultivar in Turkey is ‘Amasya’ which is a very important genetic source with a good fruit quality. In this study, it was aimed to construct especially genetic map of Amasya cultivar and to determine quantitative trait loci of fruit quality characters such as aroma compounds, sugars etc. by using a F1 population that was created by crossing ‘Kaşel-37’ (female parent; selected from Amasya cultivar) and ‘Delbarestivale’ (male parent) cultivars. 699 SSR, 240 AFLP, 121 E-STS, 24 RGA primer pairs were screened for segregation firstly in the DNAs of parents and 6 F1 plants, then segregated ones were analyzed in all F1 plants. A total of 621 markers were mapped in the genetic map of 'Kaşel-37' cultivar, and the total map was 1.700,1 cm, The average length of linkage groups was calculated 100,0 cm. A total of 17 linkage groups was located with 286 AFLP, 323 SSR, 7 E-STS, 2 RGA and 3 different characters. A total of 557 markers were mapped in the genetic map of 'Delbarestivale' cultivar, and the total map was 1.826.7 cm, The average length of linkage groups was calculated 107.5 cm. A total of 17 linkage groups was located with 254 AFLP, 390 SSR, 7 E-STS, 1 RGA and 5 different characters. In conclusion, saturated genetic maps were established for both ‘Amasya’ and ‘‘Delbarestivale’’ cultivars to use them for QTL analysis.

Keywords

Apple Amasya apple genetic map microsatellite

References

  • Conner, P.J., Brown, S.K., Weeden, N.F., 1997. Randomly amplified polymorphic DNA-based genetic linkage maps of three apple cultivars. J. Amer. Soc. for Hort. Sci., 122:350-359.
  • Doyle, J.J., Doyle, J.L., 1987. A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19:11-15.
  • FAOSTAT, 2015. Agriculture data [online]. http://faostat3.fao.org/browse/q/qc/e.
  • Fernandez, F.F., Harvey, N. G., James, C.M., 2006. Isolation and characterization of polymorphic microsatellite markers from European pear (Pyrus communis L.). Molecular Ecology Notes 6:1039–1041.
  • Grattapaglia, D., Sederoff, R., 1994. Genetic linkage maps of eucalyptus grandis and eucalyptus urophylla using a pseudo-testcross:mapping strategy and RAPD markers. Genetics 137:1121-1137.
  • Guilford, P., Prakash, S., Zhu, J.M., Rikkerink, E., Gardiner, S., Bassett, H., Forster, R., 1997. Microsatellites in Malus x domestica (apple):abundance, polymorphism and cultivar identification. Theoretical and Applied Genetics, 97:249-254.
  • Gianfranceschi, L., Seglias, N., Tarchini, R., Komjanc, M., Gessler, C., 1998. Simple sequence repeats for the genetic analysis of apple. Theoretical and Applied Genetics 96:1069-1076.
  • Han, Y., Chagne, D., Gasic, K., Rikherink, E.H.A., Beever, J.E., Gardiner, S.E., Korban, S.S., 2009. BAC-end sequence-based SNPs and Bin mapping for rapid integration of physical and genetic maps in apple. Genomics 93:282-288.
  • Han, Y., Zheng, D., Vimolmangkang, S., Khan, M.A., Beever, J.E., Korban, S.S., 2011. Integration of physical and genetic maps in
  • apple confirms whole-genome and segmental duplications in the apple genome. J. of Experimental Botany, 1-14.
  • Hemmat, M., Weeden, N.F. and Brown, S.K., 2003. Mapping and evaluation of Malus x domestica microsatellites in apple and pear. J. Amer. Soc. Hort. Sci., 128 (4):515-520.
  • Hokanson, S.C., Szewc-McFadden, A.K., Lamboy, W.F., McFerson, J.R., 1998. Microsatellite (SSR) markers reveal genetic identities, genetic diversity and relationships in a Malus x domestica borkh. Core subset collection. Theoretical and Applied Genetics 97:671683.
  • Hemmat, M., Weeden, N.F., Manganaris, A.G., Lawson, D.M., 1994. Molecular marker linkage map for apple. J. Heredity 85:4-11.
  • Inoue, E., Matsukı, Y., Anzai, H., Evans, K., 2007. Isolation and characterization of microsatellite markers in Japanese pear Molecular Ecology Notes, 7:445–447.
  • Kafkas, E., Atasay, A., Sabır, F., Akgül, H., Uçgun, K., 2010. Effects of different irrigation intervals and fertilizer applications on certain biochemical contents of ‘Breaburn’ apple cultivar. African J. of Biotechnology, 8(10):2138-2142.
  • Kafkas, S., 2006. Phylogenetic analysis of the genus Pistacia by AFLP markers. Plant Systematics and Evolution 262:113-124.
  • Kafkas, S., Özgen, M., Doğan, Y., Özcan, B., Ercişli, S., Serçe, S., 2008. Molecular characterization of mulberry accessions in Turkey by AFLP markers. J. Amer. Soc. for Hort. Sci., 13:593597.
  • Khan, M.A., Han, Y., Zhao, Y.F., Troggio, M., Korban, S.S., 2012, A Multi-population consensus genetic map reveals inconsistent marker order among maps likely attributed to structural variation in the apple genome. Plos One 7:11.
  • Kosambi, D.D., 1944. The estimation of map distances from recombination values. Annals. Eugenics 12:172-175.
  • Liebhard, R., Gianfranceschi, L., Koller, B., Ryder, C.D., Tarchini, R., Weg, Van De E., Gessler, C., 2002. Development and characterisation of 140 new microsatellites in apple. Molecular Breeding 10:217–241.
  • Liebhard, R., Koller, B., Potacchi, A., Kellerhals, M., Pfammatter, W., Jermini, M., Gessler, C., 2003. Mapping quantitative field resistance against apple scap in a ‘Fiesta’ x ‘Discovery’ progeny. Phytopathology 93:493-501.
  • Maliepaard, C., Alston, F.H., Arkel, van G., Brown, L.M., Chevreau, E., Dunemann, F., Evans, K.M., Gardiner, S., Guilford, P., Heusden, van A.W., Janse, J., Laurens, F., Lynn, J.R., Manganaris, A.G., Nijs, den A.P.M., Periam, N., Rikkerink, E., Roche, P., Ryder, C., Sansavini, S., Schmidt, H., Tartarini, S., Verhaegh, J.J., Ginkel, van- M.V., King, G.J., 1998. Aligning male and female linkage maps of apple (Malus pumila Mill.) using multiallelic markers. Theoretical and Applied Genetics 97 (1-2):60-73.
  • Naik, S., Hampson, C., Gasic, K., Bakkeren, G., Korban S.S., 2006. Development and linkage mapping of E-STS and RGA markers for functional gene homologues in apple. Genome 49:959–968.
  • Oddou-Muratorio, S., Aligon, C., Decroocq, S., Plomion, C., Lamant, T., Mush-Demesure, B., 2001. Microsatellite primers for Sorbus torminalis and related species. Molecular Ecology Notes 1:297-299.
  • Özçağıran, R., Ünal, A., Özeker, E., İsfendiyaroğlu, M., 2004. Ilıman Iklim Meyve TürleriYumuşak Çekirdekli Meyveler. Ege Üniv. Ziraat Fak. Yayınları 556, 2:7-9.
  • Silfverberg-Dilworth, E., Matasci, C.L., Van de Weg, W.E., Van Kaauwen, M.P.W., Walser, M., Kodde, L.P., Soglio, V., Gianfranceschi, L., Durel, C.E., Costa, F., Yamamoto, T., Koller, B., Gessler, C., Patocchi, A., 2006. Microsatellite markers spanning the apple (Malus x domestica Borkh.) genome. Tree Genetics & Genomes 2 (4):202-224.
  • Şahinoğlu, A.R., 2011. Bazı elma çeşitlerinde soğuklama gereksinimlerinin saptanması ve subtropik koşullara uygunluğunun incelenmesi. Yüksek Lisans Tezi. Çukurova Üniversitesi Fen Bilimleri Enstitüsü, 55s.
  • Testolin, R., Marrazzo, T., Cipriani, G., Quarta, R., Verde, I., Dettori, M.T., Pancaldi, M., Sansavini, S., 2000. Microsatellite DNA in peach (Prunus persica L. Batsch) and its use in fingerprinting and testing the genetic origin of cultivars. ProQuest Health and Medical Complete 43 (3):512.
  • Van Ooijen, J.W., 2011. JoinMap 4.1, Software for the calculation of genetic linkage maps in experimental populations. Kiyazma B.V., Wageningen, Netherlands.
  • Voorrips, R.E., 2002. Map Chart: software for the graphical presentation of linkage maps and QTLs. The Journal of Heredity 93:77-78.
  • Vos, P., Hogers, L., Bleeker, M., Van De Lee, T., Hornes, m., Frijters, A., Pot, J., Peleman, J., Kuiper, M., Zabeau, M., 1995. AFLP:A new technique for DNA fingerprinting. Nucleic Acids Research 23:4407-4414.
  • Wang, A., Aldwinckle, H., Forsline, P., Main, D., Matsuta, N., 2006b. Simple sequence repeats Fazio, G., Brown, S., Xu, K., 2012. EST for genetic analysis in pear. Euphytica. 124:contig-based SSR linkage maps for Malus x 129-137.
  • domestica cv Royal Gala and an apple scab Zhang, Q., Ma, B., Li, H., Chang, Y., Han, Y., Li, J., resistant accession of M. sieversii, the Wei, G., Zhao, S., Khan, M. A., Zhou, Y., Gu, progenitor species of domestic apple. C., Zhang, X., Han, Z., Korban, S. S., Li, S., Han, Y. ,2012. Identification, characterization, Molecular Breeding 29:379-397.
  • Yamamoto, T., Kimura, T., Shoda, M., Ban, Y., and utilization of genome-wide simple Hayashi, T., Matsuta, N., 2002a. Development sequence repeats to identify a QTL for acidity of microsatellite markers in the Japanese pear in apple. BMC Genomics 13:537.
  • Yamamoto, T., Kimura T., Sawamura, Y., Manabe, T., Kotobuki, K., Hayashi, T., Ban, Y., Mole. Eco.Notes 2:14-16.
There are 35 citations in total.

Details

Primary Language Turkish
Subjects Agricultural Engineering (Other)
Journal Section Makaleler
Authors

Murat Güney

Nergiz Çoban This is me

Mortaza Khodaeiaminjan This is me

Hayat Topçu

Ebru Kafkas

Şerif Özongun

Ayşe Nilgün Atay

Kadir Uğurtan Yılmaz

Salih Kafkas

Publication Date March 31, 2016
Submission Date January 1, 2016
Acceptance Date January 31, 2016
Published in Issue Year 2016 Volume: 45 Issue: (Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi

Cite

APA Güney, M., Çoban, N., Khodaeiaminjan, M., … Topçu, H. (2016). Amasya Elmasının SSR, AFLP, E-STS ve RGA Markörleri İle Doymuş Genetik Haritasının Oluşturulması. Bahçe, 45((Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi), 223-229.
AMA Güney M, Çoban N, Khodaeiaminjan M, et al. Amasya Elmasının SSR, AFLP, E-STS ve RGA Markörleri İle Doymuş Genetik Haritasının Oluşturulması. Bahçe. March 2016;45((Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi):223-229.
Chicago Güney, Murat, Nergiz Çoban, Mortaza Khodaeiaminjan, Hayat Topçu, Ebru Kafkas, Şerif Özongun, Ayşe Nilgün Atay, Kadir Uğurtan Yılmaz, and Salih Kafkas. “Amasya Elmasının SSR, AFLP, E-STS Ve RGA Markörleri İle Doymuş Genetik Haritasının Oluşturulması”. Bahçe 45, no. (Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi (March 2016): 223-29.
EndNote Güney M, Çoban N, Khodaeiaminjan M, Topçu H, Kafkas E, Özongun Ş, Atay AN, Yılmaz KU, Kafkas S (March 1, 2016) Amasya Elmasının SSR, AFLP, E-STS ve RGA Markörleri İle Doymuş Genetik Haritasının Oluşturulması. Bahçe 45 (Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi 223–229.
IEEE M. Güney, N. Çoban, M. Khodaeiaminjan, H. Topçu, E. Kafkas, Ş. Özongun, A. N. Atay, K. U. Yılmaz, and S. Kafkas, “Amasya Elmasının SSR, AFLP, E-STS ve RGA Markörleri İle Doymuş Genetik Haritasının Oluşturulması”, Bahçe, vol. 45, no. (Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi, pp. 223–229, 2016.
ISNAD Güney, Murat et al. “Amasya Elmasının SSR, AFLP, E-STS Ve RGA Markörleri İle Doymuş Genetik Haritasının Oluşturulması”. Bahçe 45/(Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi (March2016), 223-229.
JAMA Güney M, Çoban N, Khodaeiaminjan M, Topçu H, Kafkas E, Özongun Ş, Atay AN, Yılmaz KU, Kafkas S. Amasya Elmasının SSR, AFLP, E-STS ve RGA Markörleri İle Doymuş Genetik Haritasının Oluşturulması. Bahçe. 2016;45:223–229.
MLA Güney, Murat et al. “Amasya Elmasının SSR, AFLP, E-STS Ve RGA Markörleri İle Doymuş Genetik Haritasının Oluşturulması”. Bahçe, vol. 45, no. (Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi, 2016, pp. 223-9.
Vancouver Güney M, Çoban N, Khodaeiaminjan M, Topçu H, Kafkas E, Özongun Ş, et al. Amasya Elmasının SSR, AFLP, E-STS ve RGA Markörleri İle Doymuş Genetik Haritasının Oluşturulması. Bahçe. 2016;45((Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi):223-9.
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