Bazı Nektarin ve Erik Çeşitlerinin Embriyo Kültürleri Üzerine Besin Ortamı ve Genotipin Etkisi

Year 2016, Volume: 45 Issue: (Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi, 1142 - 1146, 31.03.2016

Müge Şahin , Celal Şafak Andaç Çavdar , Deniz Aksoy , Selay Doğan

Abstract

Çalışmada Armking, Bigtop, Caldesi nektarin (Prunus persica var. nectarine Maxim.) ve Methley japon eriği (Prunus salicina) çeşitlerinde embriyo kültürü çalışmaları gerçekleştirilmiştir. In vitro şartlarda izole edilen embriyolar BAP (0.5-1–1.5 mg/l) ve GA₃ (0.1 mg/l)’ün farklı konsantrasyon ve kombinasyonlarını içeren MS besin ortamlarında kültüre alınmıştır. Çimlenme oranları, sürgün uzunluğu, kök uzunluğu, kardeşlenme oranları ve rozet bitki oluşumu çeşitler bazında belirlenerek ortamların ve genotipin etkileri incelenmiştir. En yüksek çimlenme oranları; Armking çeşidinde kontrol ve 1.5 mg/l BAP + 0.1 mg/l GA₃ ortamında %100, Caldesi ve Methley çeşidinde 1.5 mg/l BAP + 0.1 mg/l GA₃ ortamında sırasıyla %100, %86, Bigtop çeşidinde ise besin ortamlarının tamamında %100 oranında çimlenme sağlanmıştır. Armking ve Caldesi çeşitlerinde görülen yoğun bakteriyel kontaminasyondan dolayı bu çeşitlerde sadece çimlenme oranları belirlenmiştir. Bigtop ve Methley çeşitleri sürgün uzunluğu bakımından incelendiğinde, hem sürgün ve kök uzunluğu hem de kardeşlenme oranı bakımından, sırasıyla 1.47 cm, 2.15 cm ve 2.95 adet ile Methley çeşidinden en yüksek sonuçlar elde edilmiştir. Bigtop çeşidinde 0.5 mg/l BAP + 0.1 mg/l GA₃, Methley çeşidinde ise 1 mg/l BAP + 0.1 mg/l GA₃ ve 1.5 mg/l BAP + 0.1 mg/l GA₃ içeren ortamlarda rozet bitki oluşumu gözlemlenmiştir.

Keywords

In vitro , embriyo kültürü , Prunus persica var. nectarine , Prunus salicina

Effects of Different MS Medium on Embriyo Cultures of Some Plum and Nectarin Varieties

Abstract

In this research embryo culture studies were performed in Armking, Bigtop, Caldesi nectarine varieties (Prunus persica var. nectarine Maxim.) and Methley japanese plum (Prunus salicina) variety. Embryos were isolated in-vitro conditions and were cultured in MS medium which containing different concentrations and combinations of BAP (0.5 to 1-1.5 mg/l) and GA₃ (0.1 mg/l). Two weeks after germination, germination rates, shoot and root length, tillering and rosette plant rates were determined on the basis of different medium and genotypes. The highest germination rates were identified in Armking variety with control and 1.5 mg/l BAP + 0.1 mg/l GA₃ medium 100%, Caldesi and Methley varieties with 1.5 mg/l BAP + 0.1 mg/l GA₃ medium respectively 100%, 86% and in Bigtop variety 100% germination were seen at all of the hormone concentrations. Intensive bacterial contamination was seen in Armking and Caldesi varieties for that reason only germination rates were determined in these varieties. When analyzed Methley and Bigtop varieties in terms of shoot and root length as tillering rate, the highest result was obtained in Methley variety respectively 1,47 cm, 2,15 cm and 2,95. Rosette plant formation was observed in Bigtop variety with 0.5 mg/l BAP + 0.1 mg/l GA₃ medium and in Methley variety with 1 mg/l BAP + 0.1 mg/l GA₃ and 1.5 mg/l BAP + 0.1 mg/l GA₃ medium.

Keywords

In vitro , embryo culture , Prunus persica var. nectarine , Prunus salicina

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