Türkiye’den Toplanan Kestane Kabağı (Cucurbita maxima Duchesne) Genotiplerinde Meyve Et Rengi İle Karotenoid Miktarı Arasındaki İlişkinin Belirlenmesi

Year 2016, Volume: 45 Issue: (Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi, 55 - 60, 31.03.2016

Ruhsar Yanmaz , Selen Akan , Ayşegül Salman

Abstract

Kışlık kabak ıslahında meyve et rengi önemli bir kalite kriteridir. Kabak meyvelerine et rengini ihtiva ettikleri karotenoid bileşikleri vermektedir. Kışlık kabaklarda hakim karotenoid 3-karoten olmakla birlikte, a-karoten, lutein, zeaksantin, 3-kryptoksantin, antheraksantin ve likopen de bulunmaktadır. Kestane (Cucurbita maxima Duchesne) kabağı genotiplerinde karoten içeriği ve profili ile renk arasındaki ilişkinin belirlenmesi amacıyla ülkemizin farklı bölgelerinden toplanan ve seleksiyona tabi tutulan kestane kabakları arasından sarı, turuncu ve koyu turuncu meyve et rengine sahip toplam 27 genotip denemeye alınmıştır. Hasat dönemi sonunda alınan örneklerde toplam karotenoid miktarı (mg/kg) ve karoten bileşikleri olan lutein, zeaksantin, 3-kryptoksantin ve -karoten miktarı (mg/kg) ile meyve et rengi değerleri L*, a*, b*, a/b, (a/b)², C*, h° cinsinden belirlenmiştir. Araştırmamızda meyve et rengi ve karotenoid bileşikler yönünden genotipler arası fark p<0,001 hata düzeyinde istatistiksel olarak anlamlı bulunmuştur. Meyve et rengi ile karotenoid bileşikleri arasında ise istatistiki düzeyde önemli ilişki olduğu saptanmıştır.

Keywords

Karotenoid miktarı , Cucurbita maxima Duchesne , kestane kabağı , meyve et rengi , ıslah

Determination of Relations Between Carotenoid Quantity and Flesh Color on Winter Squash (Cucurbita Maxima Duchesne) Collected from Turkey

Abstract

Flesh color is an important criterion on winter squash (Cucurbita Maxima Duchesne) breeding. Carotenoid compounds that pumpkins contain supplies a variety of color for their flesh color. Winter squashes contain mainly P-carotene also a-carotene, lutein, zeaxanthin, beta-cryptoxanthin, antheraxanthin and lycopene is available in it. Determination of relations between carotene content and color of winter squash collected from different regions of the country with subjected to selection about fruit flesh color yellow, orange and dark orange were a total of 27 genotypes experiment. At the end of the harvest period, a total carotenoid (mg/kg), lutein, zeaxanthin, beta-cryptoxanthin and beta-carotene amount (mg/kg) with fruit flesh color (L*, a*, b*, a/b, (a/b)2, C*, h°) are determined in samples. As a result of study in relationship between flesh color and carotenoid compounds has a significant correlation at the p<0,001 level. It is revealed that there is statistically significant correlation level relationship between fruit flesh color and carotenoid compounds.

Keywords

Carotenoid amount , Cucurbita maxima Duchesne , winter squash , fruit flesh color , breeding

References

• Azevedo-Meleiro, C.H., Rodriguez-Amaya, D.B., 2007. Qualitative and quantitative differences in carotenoid composition among Cucurbita moschata, Cucurbita maxima, and Cucurbita pepo. J. Agric. Food Chem., 55:4027-4033.
• Benadi, A.J., Pac. J., 2003. Clin. Nutr. 12(1):369.
• Caili, F.U., Huan, S. Quanhong L.I., 2006. A review on pharmacological activities and utilization Technologies of pumpkin. Plant Foods for HumanNut., 61 (2):73-80.
• Carvalho, L.M., Gomes, P.B., Godoy, R.L., Pacheco,S., Monte, P.H.F., Carvalho, J.L.V., Nutti, M.G., Neves, A.C.L., Vieira, A.C.R.A., Ramos, S.R.R., 2012. Total carotenoid content, a-carotene and P-carotene, of landrace pumpkins (Cucurbita moschata Duch):A preliminary study. Food Research International 47(1):337-340.
• Drammeh, B.S., Marquis, G.S., Funkhouser, E.C., Bates, Eto, I., Stephensen, C.B., 2002. J. Nutr., 132(1):3693.
• Ferruzzi, M.G., Sander, L.C., Rock, C.L., Schwartz, S.J., 1998. Carotenoid determination in biological microsamples using liquid chromatography with a coulometric electrochemical array detector. Analytical Chem., 256:74-81.
• itle, R.A., Kabelka, R.A., 2009. Correlation between L a b color space values and carotenoid content in pumpkins and squash (Cucurbita spp.). Hort Science, 44(3):633-637.
• Jin, T., Wu, Z., Liu, L., Li, X., 1999. Nır spectroscopy analysis of p-carotene and vitamin E in pumpkin. Acta Hort., 483.
• Kim, S., An, G., Yoon, S., Lee, Y., Ha, S., 2003. Korean J. Food Sci. Technol., 35(1):519.
• Liu, R.H., 2004. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J. Nutr., 134:79-85.
• Maiani, G., Periago Casto'n, M.J., Catasta, G.,Toti, E., Goni Cambrodo'n, I., Bysted, Granado-Lorencio, F., Olmedilla-Alonso, Knuthsen, P., Valoti, M., Bohm, V., Mayer-Miebach, E., Behsnilian, D., Schlemmer, U., 2009. Carotenoids: actual knowledge on food sources, intakes, stability and bioavailability and their protective role in humans. Molecular Nut. and Food Res., 53(2):194-218.
• Murkovic, M., Mülleder, U., Neunteufl, H., 2002. Carotenoid content in different varieties of pumpkins. J. Food Comp. Anal., 15(6):633-638.
• Norshazila, S., Irwandi, J., Othman, R., Yumi Zuhanis, R.R., 2014. Carotenoid content in different locality of pumpkin (Cucurbita moschata) in Malaysia. International Journal of Pharmacy and Pharma. Sci., 6(3):29-32.
• Pandey, S., Singh, J., Upadhyay, A.K., Ram, D., Rai, M., 2003. Ascorbate and carotenoid content in an Indian collection of pumpkin (Cucurbita moschata Duch.ex Poir.). Cucurbit Gen. Coop. Report, 26:51-53.
• Provesi, J.G., Dias, C.O., Amante, E.R., 2011. Changes in carotenoids during processing and storage of pumpkin puree. Food Chem., 128(1):195-202.
• Rodriguez-Amaya, D.B., Kimura, M., Godoy, H.T., Amaya-Farfan, J., 2008. Updated Brazilian database on food carotenoids: Factors affecting carotenoids composition. J. Food Comp. Anal., 21:445-463.
• Sass-Kiss, A., Kiss, J., Milotay, P., Kerek, M.M., Toth-Markus, M., 2005. Differences in anthocyanin and carotenoid content of fruits and vegetables. Food Res. Int., 38(1):1023-1029.
• Seo, S.J., Burri, B.J., Quan, Z., Neidlinger, T.R., 2005. Extraction and chromatography of carotenoids from pumpkin. J. Chromatography A. 1073(1):371-375.
• Tee, E.S., Lim, C.L., 1991. Carotenoid composition and content of Malaysian vegetables and fruits by the AOAC and HPLC Methods. Food Chem. 41:309-339.