Determination of Onion yellow dwarf virus; (OYDV) in Allium Genus Plants in South Marmara Region by Elisa Method

Year 2016, Volume: 45 Issue: (Özel Sayı 1) 7. Ulusal Bahçe Bitkileri Kongresi, 234 - 238, 31.03.2016

Hasan Tuna Tuzlalı , Ali Karanfil , Savaş Korkmaz

Abstract

Besides their economic value, onion, garlic and leek have huge importance in agricultural production due to their nutritional properties and their medicinal aromatic plant characteristics. According to 2014 statics, onion production was 1.938.225 tons, garlic production was 116.089 tons and leek production was 223.303 tons in Turkey. There a lot of diseases that restricts production of plants from Allium genus. One of these diseases is Onion yellow dwarf virus (OYDV) which causes important loses in yield and product quality. The aim of this study is to determination of the presence of OYDV in garlic, onion and leek production areas in Çanakkale, Bursa and Balıkesir provinces. As regards to this aim, a survey for OYDV infected plants was conducted in 2014-2015 production seasons. A total of 165 samples was collected including 78 leek samples, 52 onion samples and 35 garlic samples, which shows symptoms like chlorosis, dwarfing and leaf roll. These samples were tested by DAS-ELISA with using special polyclonal antibodies for OYDV. As a results of DAS-ELISA tests, out of 165 samples 68 were positive and eight was found as suspicious of OYDV.

Keywords

South Marmara Region , Onion yellow dwarf virus , OYDV , Elisa

Güney Marmara Bölgesinde Allium Cinsi Bitkilerde Soğan Sarı Cücelik Virüsünün Elisa Yöntemi İle Belirlenmesi

Abstract

Soğan (Allium cepa), sarımsak (Allium sativum) ve pırasa (Allium porrum) ekonomik değerlerinin yanı sıra besleyici özellikleri ve tıbbi aromatik bitki özelliği taşımaları nedeni ile tarımsal üretimde büyük önem taşırlar. 2014 yılı verilerine göre ülkemizde 1.938.255 ton soğan, 116.089 ton sarımsak ve 223.303 ton pırasa üretimi gerçekleştirilmiştir. Allium cinsi bitkilerin üretimini sınırlandıran birçok hastalık bulunmaktadır. Bu hastalıklardan birisi de verim ve kalite kayıplarına neden olan Soğan sarı cücelik virüsü (Onion yellow dwarf virus; OYDV)’dür. Bu çalışmada Çanakkale, Bursa ve Balıkesir illerinde pırasa, sarımsak ve soğan üretimi yapılan alanlarda OYDV’nin varlığının belirlenmesi amaçlanmıştır. Bu bağlamda 2014-2015 üretim sezonu içerisinde bir sörvey gerçekleştirilerek OYDV ile infekteli olduğu düşünülen ve kloroz, bodurluk, yapraklarda kıvrılma ve bükülme belirtileri gösteren 78’i pırasa, 52’si soğan ve 35’i sarımsak olmak üzere toplam 165 bitki toplanmış ve OYDV’ye özgü ticari poliklonal antibadiler kullanılarak DAS-ELISA ile test edilmiştir. DAS-ELISA testleri sonucunda 165 örnekten 68’i pozitif olarak saptanırken, 8 örnek ise şüpheli olarak değerlendirilmiştir.

Keywords

Güney Marmara Bölgesi , Soğan sarı cücelik virüsü , OYDV , Elisa

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