Anthurium andraeanum in vitro Kültüründe Besin Ortamı pH’ının Sürgün Rejenerasyonuna ve Gelişimine Etkisi

Year 2025, Volume: 54 Issue: Özel Sayı 1, 104 - 110, 25.03.2025

Cansu Dindar , Merve Kabakcı , Uğur Şirin , Mustafa Ercan Özzambak

https://doi.org/10.53471/bahce.1559705

Abstract

Süs bitkileri grupları arasında önemli bir yer tutan iç mekân süs bitkilerinden olan Anthurium sp. vejetatif ve generatif olarak çoğaltılabilmekte ancak bu işlemler uzun zaman almaktadır. Aynı zamanda ticari bir üretim için gereken standardizasyonu sağlamakta da güçlük görülmektedir. Kitlesel seri üretim olanaklarından olan in vitro kültürde iyi bir çoğaltım protokolü geliştirmek ticari açıdan değer taşımaktadır. Doku kültürü ile üretimde bitkilerin, besin maddeleri alınımında etkili olan pH aralıkları çoğunlukla 5,6-5,8 değerleri arasında olsa da bazı bitkilerin asidik, bazı bitkilerin bazik ortamlarda iyi geliştiği göz önünde bulundurulunca besin ortamının da farklı pH değerlerinde ayarlanması ile bitki gelişimini gözlemlemek, geliştirilecek protokollerde fayda sağlayacaktır. Bu bağlamda, bu araştırmada Anthurium andraeanum’un in vitro kültüründe MS besin ortamının pH’ı 4 farklı değer (4,3, 4,8, 5,3 ve 5,8) olarak ayarlanmıştır. Araştırma sonucunda; eksplant başına oluşan yeni sürgün sayısı 40,33 adet ile kontrol grubunda (pH 5,8) elde edilirken, en düşük değer aynı istatistiki grupta yer alan, 4,3, 4,8, 5,3 pH değerlerinden sırasıyla 3,67 adet, 7 adet, 9 adet olarak elde edilmiştir. 4,3 pH’a sahip ortamda eksplant başına oluşan yeni sürgün sayısı en düşük değeri alırken, aynı ortam, eksplantta gelişen yeni sürgün uzunluğu bakımından en yüksek sonuçları vermiştir. Ölçülen diğer parametrelerde istatistiki olarak bir farklılık belirlenmemiştir.

Keywords

in vitro, doku kültürü, pH, Anthurium sp.

Effect of pH Level of Culture Media on Shoot Regeneration in in vitro Culture of Anthurium sp.

Abstract

Anthurium sp., one of the indoor ornamental plants, which has an important place among ornamental plant groups, can be propagated vegetatively and generatively, but these processes take a long time. At the same time, it is difficult to provide the standardization required for commercial production. Developing a good propagation protocol in in vitro culture, which is one of the mass production possibilities, is of commercial value. Although the pH ranges that are effective in the nutrient uptake of plants in tissue culture production are mostly between 5,6-5,8 values, considering that some plants grow well in acidic and some plants grow well in basic environments, it will be beneficial to observe plant growth by adjusting the nutrient medium at different pH values in the protocols to be developed. In this context, in this study, the pH of MS nutrient medium was adjusted to 4 different values (4,3, 4,8, 5,3 and 5,8) in in vitro culture of Anthurium andraeanum. As a result of the research, the number of new shoots formed per explant was 40,33 in the control group (pH 5,8), while the lowest value was obtained as 3,67, 7 and 9 in the same statistical group with pH values of 4,3, 4,8 and 5,3, respectively. At pH 4,3, the number of new shoots per explant was the lowest value, while the same medium gave the highest results in terms of the length of new shoots per explant. There was no statistical difference in other measured parameters.

Keywords

in vitro, tissue culture, pH, Anthurium sp.

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