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Potasyum (K) Çözücü Bakteri (Frateuria aurantia) İçerikli Mikrobiyal Gübre Uygulamalarının Salata (Lactuca sativa) Kimi Büyüme, Kalite ve Verim Özelliklerine Etkisi

Year 2022, Volume: 51 Issue: (Özel Sayı 1) 13. Sebze Tarımı Sempozyumu, 214 - 219, 19.12.2022

Abstract

Bu araştırmada, aktif bileşeni potasyum (K) çözücü özellikli Frateuria aurantia bakterisi olan mikrobiyal gübrenin organik gübre ile kullanımının marul yetiştiriciliğine etkisinin ortaya konması amaçlanmıştır. Deneme, İzmir-Ödemiş’te 4 tekrarlamalı tesadüf blokları deneme desenine göre yürütülmüştür. Cartagenas RZ iceberg salata çeşidinin kullanıldığı çalışmada kontrol (100 L da⁻¹ gübresiz su), organik gübre (100 kg da⁻¹), mikrobiyal gübre (300 ml da⁻¹/100 L su) ve mikrobiyal gübre (300 ml da⁻¹) + organik gübre (100 kg da⁻¹) olmak üzere 4 uygulama karşılaştırılmıştır. Mikrobiyal gübre uygulamalarının bitki boyu, yaprak sayısı, kök uzunluğu, gövde çapı ve yaprak alan indeksi üzerinde etkisinin olduğu gözlenmiştir. En yüksek bitki gelişim değerleri “300 ml da⁻¹” mikrobiyal gübre 100 kg da⁻¹ organik gübre” uygulamasında ölçülmüştür. İstatistiki değerlendirmeler su ve organik madde ile mikrobiyal gübre uygulamalarının kontrol parsellerine göre bitki boyu ve yaprak alan indeksi üzerinde önemli düzeyde etkili olduğunu göstermiştir. Mikrobiyal gübrenin su ve organik madde ile uygulamalarının benzer etki gösterdiği ortaya çıkmıştır. Sonuç olarak; potasyum çözücü bakteri içerikli mikrobiyal gübrenin organik gübre ile birlikte uygulanması; kalite ve verim artırıcı etkisi nedeniyle önerilebilir.

References

  • Kirkby, E. 2012. Introduction, Definition and Classification of Nutrients. In:Marschner’s Mineral Nutrition of Higher Plants Third Edition, Edt. Petra Marschner. Academic Press, USA.
  • Engels, C., Kirkby, E., White, P. 2012. Mineral Nutrition, Yield and Source-Sink Relationships, In:Marschner’s Mineral Nutrition of Higher Plants Third Edition, Ed. Petra Marschner. pp:85-133, Academic Press, USA. ISBN 9780-1238-49052 (doi/10.1016/B978-0-12-384905-2.00005-4).
  • Zhang, C., Kong, F. 2014. Isolation and identification of potassium-solubilizing bacteria from tobacco rhizospheric soil and their effect on tobacco plants. Appl. Soil Ecol. 82:18-25.
  • Sheng, X.F., Huang, W.Y. 2002. Mechanism of potassium release from feldspar affected by the strain NBT of silicate bacterium. Acta Pedol Sin 39(6):863-871.
  • Meena, O.P., Maurya, B.R., Meena, V.S. 2013. Influence of K solubilizing bacteria on release of potassium from waste mica. Agric. Sustain Dev 1(1):53-56.
  • Maurya, B.R., Meena, V.S., Meena, O.P. 2014. Influence of Inceptisol and Alfisol’s potassium solubilizing bacteria (KSB) isolates on release of K from waste mica. Vegetos 27(1):181-187.
  • Barre, P., Montagnier, C., Chenu, C., Abbadie, L., Velde, B. 2008. Clay minerals as a soil potassium reservoir:observation and quantification through X-ray diffraction. Plant Soil 302:213-220.
  • Öztekin, G.B., Y. Tüzel, M. Ece 2016. Potasyum çözücü bakteri aşılamasının sera domates yetiştiriciliğinde bitki gelişimi, verim ve meyve kalitesi üzerine etkileri. Turk J. Agric. Res. 3:41-47.
  • Kumar, S., Sindhu, D.S.S., Kumar, R. 2022. Biofertilizers:An ecofriendly technology for nutrient recycling and environmental sustainability. Current Research in Microbial Sciences 3:100094.
  • Lian, B., Wang, B., Pan, M., Liu, C., Teng, H.H. 2008. Microbial release of potassium from K-bearing minerals by thermophilic fungus Aspergillus fumigatus. Geochim Cosmochim Acta 72:87-98.
  • Biswas, D.R., Basak, B.B. 2009. Influence of potassium solubilizing microorganism (Bacillus mucilaginosus) and waste mica on potassium uptake dynamics by Sudan grass (Sorghum vulgare Pers.) grown under two Alfisols. Plant and Soil 317:235-255.
  • Sheng XF. 2005. Growth promotion and increased potassium uptake of cotton and rape by a potassium releasing strain of Bacillus edaphicus. Soil Biol. Biochem. 37:1918-1922.
  • Liu, D., Lian, B., Dong, H. 2012. Isolation of Paenibacillus sp. and assessment of its potential for enhancing mineral weathering. Geomicrobiol J. 29:413-421.
  • Meena, V.S., Maurya, B.R., Bahadur, I. 2014-a. Potassium solubilization by bacterial strain in waste mica. Bangladesh J. Bot. 43(2):235-237.
  • Meena, V.S., Maurya, B.R., Verma, J.P. 2014-b. Does a rhizospheric microorganism enhance K+ availability in agricultural soils? Microbiol Res 169:337-347.
  • Kumar, A., Bahadur, I., Maurya, B.R., Raghuwanshi, R., Meena, V.S., Singh, D.K., Dixit, J. 2015. Does a plant growth-promoting rhizobacteria enhance agricultural sustainability? J. Pure Appl. Microbiol. 9(1):715-724.
  • Pindi, P.K., Satyanarayana, S.D.V. 2012. Liquid microbial consortium- a potential tool for sustainable soil health. J. Biofertil Biopesticide 3(4):1-9.
  • Bahadur, I., Maurya, B.R., Kumar, A., Meena, V.S., Raghuwanshi, R. 2016. Towards the soil sustainability and potassium-solubilizing microorganisms In:Potassium Solubilizing Microorganisms for Sustainable Agriculture, Springer India 2016 V.S. Meena et al. (eds.), (doi:10.1007/978-81-322-2776-2-18).
  • Patel, B.C. 2011. Advance method of preparation of bacterial formulation using potash mobilizing bacteria that mobilize potash and make it available to crop plant. WIPO Patent Application WO/2011/154961.
  • Han, H.S., Supanjani, Lee, K.D. 2006. Effect of co-inoculation with phosphate and potassium solubilizing bacteria on mineral uptake and growth of pepper and cucumber. Plant Soil Environ 52:130-136.
  • Sheng, X.F., He, L.Y. 2006. Solubilization of potassium bearing minerals by a wild type strain of Bacillus edaphicus and its mutants and increased potassium uptake by wheat. Can J. Microbiol 52(1):66-72.
  • Singh, G., Biswas, D.R., Marwah, T.S. 2010. Mobilization of potassium from waste mica by plant growth promoting rhizobacteria and its assimilation by maize (Zea mays) and wheat (Triticum aestivum L.). J. Plant Nutr 33:1236-1251.
  • Basak, B.B., Biswas, D.R. 2010. Co-inoculation of potassium solubilizing and nitrogen fixing bacteria on solubilization of waste mica and their effect on growth promotion and nutrient acquisition by a forage crop. Biol. Fertil. Soils 46:641-648.
  • Subhashini, D.V. 2015. Growth promotion and increased potassium uptake of tobacco by potassium mobilizing bacterium Frateuria aurantia grown at different potassium levels in vertisols. Commun Soil Sci. Plant Anal. 46:210-220.
  • Nayak B. 2001. Uptake of potash by different plants with the use of potash mobilizing bacteria Frateuria aurantia. M.Sc. (Agric) thesis, QUAT, Bhubaneswar.
  • Ramarethinam, S., Chandra, K. 2005. Studies on the effect of potash solubilizing/mobilizing bacteria Frateuria aurantia on brinjal growth and yield. Pestology 11:35-39.
  • Rodriguez, H., Fraga, R., 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances 17(4-5):319-339.
  • Rogers, G., Titley, M., Giggins, B., Bauer, B., Poynton, R., Kocks, A., McAuliffe, T., Le Budd, J. 2006. Post-harvest improvement in iceberg and cos lettuce to extend shelf life for fresh cut salads. Project Number:VG03092, Horticultural Australia Ltd. Pub. Sydney, Australia.
  • Pratt, P.F. 1965. Potassium. Editor C. A. Black, Methods of Soil Analysis Part II. American Society of Agronomy Inc., Publisher Madion, Winconsin, USA, 1022pp.
  • Kacar, B., 2009. Toprak analizleri. Nobel Yayın No:1387, 467s.
  • Chamangasht, S., Ardakani, M.R., Khavazi, K., Abbaszadeh, B., Mafakheri, S. 2012. Improving lettuce (Lactuca sativa L.) growth and yield by the application of biofertilizers. Annals of Biological Research, 3(4):1876-1879.
  • Aini, N., Sumiya Dwi Yamika, W., Ulum, B. 2019. Effect of nutrient concentration, PGPR and AMF on plant growth, yield, and nutrient uptake of hydroponic lettuce. Intl. J. Agric. Biol. 21(1).
  • Sottero NA, Freitas SS, Melo AMT, Trani PE. 2006. Rizobactérias e Alface:Colonização Rizosférica, Promoção de Crescimento e Controle Biológico. R. Bras. Ci. Solo, 30:225-234.
  • Fortt, J., González, M., Morales, P., Araya, N., Remonsellez, F., de la Peña, T.C., Ostria-Gallardo, E., Stoll, A., 2022. Bacterial modulation of the plant ethylene signaling pathway improves tolerance to salt stress in lettuce (Lactuca sativa L.). Front. Sustain. Food Syst. 6:768250. (doi:10.3389/fsufs.2022.768250).
  • Blanco, B.M., Vejar, V.A., Bello-Martínez, J., Palemón, F.A., Ramírez, Y.R. Díaz, D.O., Jiménez, J.T. 2020. Use of plant growth promoting bacteria to increase the production of Lactuca sativa L. in the field. Revista Mexicana Ciencias Agrícolas 11(2).
  • Yildirim, E., Turan, M., Ekinci, M., Dursun, A., Cakmakci, R. 2011. Plant growth promoting rhizobacteria ameliorate deleterious effect of salt stress on lettuce. Sci. Res Essays 6:4389-4396.

The Effects of Microbial Fertilizer Containing Potassium (K) Solubilizing Bacteria Treatments on Some Growth, Quality and Yield Characteristics of Lettuce (Lactuca sativa)

Year 2022, Volume: 51 Issue: (Özel Sayı 1) 13. Sebze Tarımı Sempozyumu, 214 - 219, 19.12.2022

Abstract

The objective of this study was to investigate the effects of microbial fertilizer [in which the effective part is potassium (K) solubilizing bacteria (Frateuria aurantia)] treatments with organic fertilizer on growth (length of aerial parts and root, stem diameter, dry weight of aerial parts and root), quality (number of leaves, leaf area index) and yield of lettuce. For this purpose, an experiment was planned to compare 4 different treatments for lettuce (Cartagenas RZ) production. The experiment was conducted with a factorial experiment design and 4 replications in the Ege Region (İzmir, Ödemiş) of Turkey for 58 days where a Mediterranean climate is dominant. Treatments include. control (100 L da⁻¹ water without any fertilizer), organic fertilizer (100 kg da⁻¹), microbial fertilizer (300 ml da⁻¹/100 L water), and microbial fertilizer (300 ml da⁻¹) + organic fertilizer (100 kg da⁻¹) groups. At the end of the experiment, it was seen that microbial fertilizer treatment had positive effects on the length of aerial parts and roots, leaf number, stem width, and leaf area index. The highest plant growth values were found with the “microbial fertilizer 100 kg da⁻¹ organic fertilizer” treatment. Statistical analysis proves that microbial fertilizer treatment with water and organic fertilizer has significant effects on the length of aerial parts and leaf area index compared to the control group. It is also seen that the treatment of microbial fertilizer with water and organic fertilizer has a similar effect. In conclusion; microbial fertilizer that is containing K solubilizing bacteria with organic fertilizer treatment can be recommended since its positive effect on better quality and higher yield.

References

  • Kirkby, E. 2012. Introduction, Definition and Classification of Nutrients. In:Marschner’s Mineral Nutrition of Higher Plants Third Edition, Edt. Petra Marschner. Academic Press, USA.
  • Engels, C., Kirkby, E., White, P. 2012. Mineral Nutrition, Yield and Source-Sink Relationships, In:Marschner’s Mineral Nutrition of Higher Plants Third Edition, Ed. Petra Marschner. pp:85-133, Academic Press, USA. ISBN 9780-1238-49052 (doi/10.1016/B978-0-12-384905-2.00005-4).
  • Zhang, C., Kong, F. 2014. Isolation and identification of potassium-solubilizing bacteria from tobacco rhizospheric soil and their effect on tobacco plants. Appl. Soil Ecol. 82:18-25.
  • Sheng, X.F., Huang, W.Y. 2002. Mechanism of potassium release from feldspar affected by the strain NBT of silicate bacterium. Acta Pedol Sin 39(6):863-871.
  • Meena, O.P., Maurya, B.R., Meena, V.S. 2013. Influence of K solubilizing bacteria on release of potassium from waste mica. Agric. Sustain Dev 1(1):53-56.
  • Maurya, B.R., Meena, V.S., Meena, O.P. 2014. Influence of Inceptisol and Alfisol’s potassium solubilizing bacteria (KSB) isolates on release of K from waste mica. Vegetos 27(1):181-187.
  • Barre, P., Montagnier, C., Chenu, C., Abbadie, L., Velde, B. 2008. Clay minerals as a soil potassium reservoir:observation and quantification through X-ray diffraction. Plant Soil 302:213-220.
  • Öztekin, G.B., Y. Tüzel, M. Ece 2016. Potasyum çözücü bakteri aşılamasının sera domates yetiştiriciliğinde bitki gelişimi, verim ve meyve kalitesi üzerine etkileri. Turk J. Agric. Res. 3:41-47.
  • Kumar, S., Sindhu, D.S.S., Kumar, R. 2022. Biofertilizers:An ecofriendly technology for nutrient recycling and environmental sustainability. Current Research in Microbial Sciences 3:100094.
  • Lian, B., Wang, B., Pan, M., Liu, C., Teng, H.H. 2008. Microbial release of potassium from K-bearing minerals by thermophilic fungus Aspergillus fumigatus. Geochim Cosmochim Acta 72:87-98.
  • Biswas, D.R., Basak, B.B. 2009. Influence of potassium solubilizing microorganism (Bacillus mucilaginosus) and waste mica on potassium uptake dynamics by Sudan grass (Sorghum vulgare Pers.) grown under two Alfisols. Plant and Soil 317:235-255.
  • Sheng XF. 2005. Growth promotion and increased potassium uptake of cotton and rape by a potassium releasing strain of Bacillus edaphicus. Soil Biol. Biochem. 37:1918-1922.
  • Liu, D., Lian, B., Dong, H. 2012. Isolation of Paenibacillus sp. and assessment of its potential for enhancing mineral weathering. Geomicrobiol J. 29:413-421.
  • Meena, V.S., Maurya, B.R., Bahadur, I. 2014-a. Potassium solubilization by bacterial strain in waste mica. Bangladesh J. Bot. 43(2):235-237.
  • Meena, V.S., Maurya, B.R., Verma, J.P. 2014-b. Does a rhizospheric microorganism enhance K+ availability in agricultural soils? Microbiol Res 169:337-347.
  • Kumar, A., Bahadur, I., Maurya, B.R., Raghuwanshi, R., Meena, V.S., Singh, D.K., Dixit, J. 2015. Does a plant growth-promoting rhizobacteria enhance agricultural sustainability? J. Pure Appl. Microbiol. 9(1):715-724.
  • Pindi, P.K., Satyanarayana, S.D.V. 2012. Liquid microbial consortium- a potential tool for sustainable soil health. J. Biofertil Biopesticide 3(4):1-9.
  • Bahadur, I., Maurya, B.R., Kumar, A., Meena, V.S., Raghuwanshi, R. 2016. Towards the soil sustainability and potassium-solubilizing microorganisms In:Potassium Solubilizing Microorganisms for Sustainable Agriculture, Springer India 2016 V.S. Meena et al. (eds.), (doi:10.1007/978-81-322-2776-2-18).
  • Patel, B.C. 2011. Advance method of preparation of bacterial formulation using potash mobilizing bacteria that mobilize potash and make it available to crop plant. WIPO Patent Application WO/2011/154961.
  • Han, H.S., Supanjani, Lee, K.D. 2006. Effect of co-inoculation with phosphate and potassium solubilizing bacteria on mineral uptake and growth of pepper and cucumber. Plant Soil Environ 52:130-136.
  • Sheng, X.F., He, L.Y. 2006. Solubilization of potassium bearing minerals by a wild type strain of Bacillus edaphicus and its mutants and increased potassium uptake by wheat. Can J. Microbiol 52(1):66-72.
  • Singh, G., Biswas, D.R., Marwah, T.S. 2010. Mobilization of potassium from waste mica by plant growth promoting rhizobacteria and its assimilation by maize (Zea mays) and wheat (Triticum aestivum L.). J. Plant Nutr 33:1236-1251.
  • Basak, B.B., Biswas, D.R. 2010. Co-inoculation of potassium solubilizing and nitrogen fixing bacteria on solubilization of waste mica and their effect on growth promotion and nutrient acquisition by a forage crop. Biol. Fertil. Soils 46:641-648.
  • Subhashini, D.V. 2015. Growth promotion and increased potassium uptake of tobacco by potassium mobilizing bacterium Frateuria aurantia grown at different potassium levels in vertisols. Commun Soil Sci. Plant Anal. 46:210-220.
  • Nayak B. 2001. Uptake of potash by different plants with the use of potash mobilizing bacteria Frateuria aurantia. M.Sc. (Agric) thesis, QUAT, Bhubaneswar.
  • Ramarethinam, S., Chandra, K. 2005. Studies on the effect of potash solubilizing/mobilizing bacteria Frateuria aurantia on brinjal growth and yield. Pestology 11:35-39.
  • Rodriguez, H., Fraga, R., 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances 17(4-5):319-339.
  • Rogers, G., Titley, M., Giggins, B., Bauer, B., Poynton, R., Kocks, A., McAuliffe, T., Le Budd, J. 2006. Post-harvest improvement in iceberg and cos lettuce to extend shelf life for fresh cut salads. Project Number:VG03092, Horticultural Australia Ltd. Pub. Sydney, Australia.
  • Pratt, P.F. 1965. Potassium. Editor C. A. Black, Methods of Soil Analysis Part II. American Society of Agronomy Inc., Publisher Madion, Winconsin, USA, 1022pp.
  • Kacar, B., 2009. Toprak analizleri. Nobel Yayın No:1387, 467s.
  • Chamangasht, S., Ardakani, M.R., Khavazi, K., Abbaszadeh, B., Mafakheri, S. 2012. Improving lettuce (Lactuca sativa L.) growth and yield by the application of biofertilizers. Annals of Biological Research, 3(4):1876-1879.
  • Aini, N., Sumiya Dwi Yamika, W., Ulum, B. 2019. Effect of nutrient concentration, PGPR and AMF on plant growth, yield, and nutrient uptake of hydroponic lettuce. Intl. J. Agric. Biol. 21(1).
  • Sottero NA, Freitas SS, Melo AMT, Trani PE. 2006. Rizobactérias e Alface:Colonização Rizosférica, Promoção de Crescimento e Controle Biológico. R. Bras. Ci. Solo, 30:225-234.
  • Fortt, J., González, M., Morales, P., Araya, N., Remonsellez, F., de la Peña, T.C., Ostria-Gallardo, E., Stoll, A., 2022. Bacterial modulation of the plant ethylene signaling pathway improves tolerance to salt stress in lettuce (Lactuca sativa L.). Front. Sustain. Food Syst. 6:768250. (doi:10.3389/fsufs.2022.768250).
  • Blanco, B.M., Vejar, V.A., Bello-Martínez, J., Palemón, F.A., Ramírez, Y.R. Díaz, D.O., Jiménez, J.T. 2020. Use of plant growth promoting bacteria to increase the production of Lactuca sativa L. in the field. Revista Mexicana Ciencias Agrícolas 11(2).
  • Yildirim, E., Turan, M., Ekinci, M., Dursun, A., Cakmakci, R. 2011. Plant growth promoting rhizobacteria ameliorate deleterious effect of salt stress on lettuce. Sci. Res Essays 6:4389-4396.
There are 36 citations in total.

Details

Primary Language Turkish
Subjects Agricultural Engineering (Other)
Journal Section Makaleler
Authors

Neriman Tuba Barlas

Publication Date December 19, 2022
Submission Date January 1, 2022
Acceptance Date January 31, 2022
Published in Issue Year 2022 Volume: 51 Issue: (Özel Sayı 1) 13. Sebze Tarımı Sempozyumu

Cite

APA Barlas, N. T. (2022). Potasyum (K) Çözücü Bakteri (Frateuria aurantia) İçerikli Mikrobiyal Gübre Uygulamalarının Salata (Lactuca sativa) Kimi Büyüme, Kalite ve Verim Özelliklerine Etkisi. Bahçe, 51((Özel Sayı 1) 13. Sebze Tarımı Sempozyumu), 214-219.
AMA Barlas NT. Potasyum (K) Çözücü Bakteri (Frateuria aurantia) İçerikli Mikrobiyal Gübre Uygulamalarının Salata (Lactuca sativa) Kimi Büyüme, Kalite ve Verim Özelliklerine Etkisi. Bahçe. December 2022;51((Özel Sayı 1) 13. Sebze Tarımı Sempozyumu):214-219.
Chicago Barlas, Neriman Tuba. “Potasyum (K) Çözücü Bakteri (Frateuria Aurantia) İçerikli Mikrobiyal Gübre Uygulamalarının Salata (Lactuca Sativa) Kimi Büyüme, Kalite Ve Verim Özelliklerine Etkisi”. Bahçe 51, no. (Özel Sayı 1) 13. Sebze Tarımı Sempozyumu (December 2022): 214-19.
EndNote Barlas NT (December 1, 2022) Potasyum (K) Çözücü Bakteri (Frateuria aurantia) İçerikli Mikrobiyal Gübre Uygulamalarının Salata (Lactuca sativa) Kimi Büyüme, Kalite ve Verim Özelliklerine Etkisi. Bahçe 51 (Özel Sayı 1) 13. Sebze Tarımı Sempozyumu 214–219.
IEEE N. T. Barlas, “Potasyum (K) Çözücü Bakteri (Frateuria aurantia) İçerikli Mikrobiyal Gübre Uygulamalarının Salata (Lactuca sativa) Kimi Büyüme, Kalite ve Verim Özelliklerine Etkisi”, Bahçe, vol. 51, no. (Özel Sayı 1) 13. Sebze Tarımı Sempozyumu, pp. 214–219, 2022.
ISNAD Barlas, Neriman Tuba. “Potasyum (K) Çözücü Bakteri (Frateuria Aurantia) İçerikli Mikrobiyal Gübre Uygulamalarının Salata (Lactuca Sativa) Kimi Büyüme, Kalite Ve Verim Özelliklerine Etkisi”. Bahçe 51/(Özel Sayı 1) 13. Sebze Tarımı Sempozyumu (December2022), 214-219.
JAMA Barlas NT. Potasyum (K) Çözücü Bakteri (Frateuria aurantia) İçerikli Mikrobiyal Gübre Uygulamalarının Salata (Lactuca sativa) Kimi Büyüme, Kalite ve Verim Özelliklerine Etkisi. Bahçe. 2022;51:214–219.
MLA Barlas, Neriman Tuba. “Potasyum (K) Çözücü Bakteri (Frateuria Aurantia) İçerikli Mikrobiyal Gübre Uygulamalarının Salata (Lactuca Sativa) Kimi Büyüme, Kalite Ve Verim Özelliklerine Etkisi”. Bahçe, vol. 51, no. (Özel Sayı 1) 13. Sebze Tarımı Sempozyumu, 2022, pp. 214-9.
Vancouver Barlas NT. Potasyum (K) Çözücü Bakteri (Frateuria aurantia) İçerikli Mikrobiyal Gübre Uygulamalarının Salata (Lactuca sativa) Kimi Büyüme, Kalite ve Verim Özelliklerine Etkisi. Bahçe. 2022;51((Özel Sayı 1) 13. Sebze Tarımı Sempozyumu):214-9.

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