MicroRNA’ların Domates Bitkisinde Abiyotik Stres Faktörlerine Karşı Toleranslığa Etkisi

Year 2022, Volume: 51 Issue: (Özel Sayı 1) 13. Sebze Tarımı Sempozyumu, 353 - 357, 19.12.2022

Halim Can Kayıkçi , Adem Kaba , İnanç Soylu , Nedim Mutlu

Abstract

21-24 nt uzunluğunda kodlanmayan RNA’lar arasında bulunan mikro RNA’lar çeşitli gen regülasyonların da aktif bir şekilde rol almaktadırlar. Bu husus özellikle stres anlarında meydana gelmekte olup bitki tolerantlığında/dayanımında olumlu veya olumsuz olarak rol oynamaktadırlar. Bu sebeple bitki ıslahında kullanımı önemli bir potansiyele sahiptir. Özellikle biyoteknolojinin de gelişmesiyle mikro RNA aracılığı ile genomda çeşitli düzenlemeler yapılabilmekte olup CRISPR, Prime editing gibi teknolojiler ile beraber direkt olarak MIR geneleri hedeflenip düzenleme de yapılabilmektedir. Bu sayede model bitkilerin yanında ekonomik değeri yüksek bitkilerin ıslahında da önemli bir araç olarak da kullanılabilmektedir. Dünya üzerinde en çok üretilen sebzelerin başında gelen domates bu türlerden birisidir. Bu makalede mikro RNA moleküllerinin abiyotik stres faktörleri özelinde domates bitkisinde kullanım olanakları tartışılmıştır.

Keywords

Domates , abiyotik stres faktörleri , mikro RNA , kısa kodlanmayan RNA’lar

The Effects of MicroRNAs on Tolerance of Abiotic Stress Factors in Tomato Plant

Abstract

Micro RNAs are among the 21-24 nt long non-coding RNAs, playing an active role in various gene regulation. This happens especially when plants are exposed to stress factors, leading to positive or negative regulations. Therefore, miRNAs have an important potential. Nowadays, micro-RNA based gene regulation can be manipulated in the genome. Furthermore, through the today’s technological tools such as CRISPR and prime editing, Micro RNA coding genes (MIR) can be up or down regulated. Thus, in addition to model crops, economically important crops can also be improved easily thanks to these tools. Tomato, one of the most produced vegetables is among these plants. In this article, the possibilities of using micro-RNA molecules in tomato plants will be discussed in terms of abiotic stress factors.

Keywords

Tomato , abiotic stress factors , micro RNAs , small non-coding RNAs

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