Achillea biebersteinii ve A. millefolium Bitkilerinin Fitokimyasal İçeriği ve In Silico Moleküler Doking Çalışmaları

Year 2024, , 10 - 24, 31.08.2024

Fatih Gül , Yunus Başar , İbrahim Demirtas

https://doi.org/10.53445/batd.1513403

Abstract

Achillea L. cinsi, damarlı bitkilerin en büyük familyası olan Asteraceae familyasına aittir. Achillea türleri, dünya çapında yaygın olarak bulunan, içerdiği biyoaktif bileşikler nedeniyle geleneksel tıpta yaygın olarak kullanılan tıbbi bir bitkidir. Bu çalışmada Iğdır Üniversitesi kampüsü arazisinde yetişen iki Achillea türünün fitokimyasal içeriğini analiz ederek bitkilerin biyolojik aktivite potansiyelini incelemeyi amaçladık. Bu çalışmada, farklı dönemlerde hasat edilen A. biebersteinii ve A. millefolium'un çiçek, gövde-yaprak kısımlarının uçucu ve fenolik içerikleri kromatografik yöntemlerle (GC-MS/MS ve LC-MS/MS) belirlendi. GC-MS analizi sonucunda A. millefolium bitkisinde yüksek düzeyde eucalyptol (%43.22) varlığının olduğu ve hasat zamanının A. biebersteinii bitkisinde uçucu bileşenlerin oranlarını önemli ölçüde değiştiği görüldü. LC-MS/MS analizinde, tüm bitki materyallerindeki ana bileşenin klorojenik asit olduğunu gösterdi. Ayrıca ana bileşen, klorojenik asit ile ksantin oksidaz enzimi etkileşimleri, in silico moleküler yerleştirme yöntemi ile belirlendi. Ksantin oksidaz ile klorojenik asit etkileşiminin, bağlanma enerjisi (-8,0 kcal/mol) ve MolDock skoru (-130,96) hesaplandı.

Keywords

A. biebersteinii, A. millefolium, Fitokimyasal analiz, Klorojenik asit, Ksantin oksidaz

Project Number

YİP0723İ01

Phytochemical Content and In Silico Molecular Docking Studies of Achillea biebersteinii and A. millefolium Plants

Abstract

The genus Achillea L. belongs to Asteraceae, the largest family of vascular plants. Achillea species is a medicinal plant widely used in traditional medicine due to the bioactive compounds it contains that are widely distributed worldwide. In this study, we aimed to examine the biological activity potential of the plants by analyzing the phytochemical content of two Achillea species growing on the land of Iğdır University campus. In this study, the volatile and phenolic contents of flower, and stem-leaf parts of A. biebersteinii and A. millefolium harvested in different periods were determined by chromatographic methods (GC-MS/MS and LC-MS/MS). As a result of the GC-MS analysis, it was observed that the presence of high levels of eucalyptol (43.22%) in the A. millefolium plant and that the harvest time dramatically changed the rates of volatile components in the A. biebersteinii plants. The LC-MS/MS analysis showed that the main constituent in all plant materials was chlorogenic acid. In addition, the main component, chlorogenic acid interactions with the xanthine oxidase enzyme were determined by in silico molecular docking. Chlorogenic acid interaction with xanthine oxidase was calculated of binding energies (-8.0 kcal/mol) and MolDock score (-130.96).

Keywords

A. biebersteinii, A. millefolium, Phytochemical analysis, Chlorogenic acid, Xanthine oxidase

Supporting Institution

Yüksek Öğretim Kurumu (YÖK)

Project Number

YİP0723İ01

Thanks

The High-Value Added Agricultural Products Specialization Program, Production of Value-Added Raw Materials from Agricultural Products by Supercritical Carbon Dioxide Extraction Method (Project No: YİP0723İ01).

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