Depresyon ve anksiyete tedavisi gören hastalarda comet testi kullanılarak olası genotoksik hasarın belirlenmesi

Abstract

Anksiyete ve depresyon, günümüzde çok sık görülen rahatsızlıklar olup sıklıkla birlikte görülmektedir. Bu çalışma, comet testi kullanılarak depresyon ve anksiyete bozukluğu tanısı alan ve tedavi edilen hastalarda DNA hasarını ölçmek için tasarlanmıştır. Anksiyete ve depresyon nedeniyle farmakolojik tedavi (seçici serotonin geri alım inhibitörleri-SSRI'lar, seçici norepinefrin geri alım inhibitörleri-SNRI'lar ve antipsikotikler) alan otuz sekiz hasta ve yirmi sekiz sağlıklı gönüllü çalışmaya dahil edilmiştir. DNA hasarını değerlendirmek için periferal kan lenfositlerinde comet testi kullanılmıştır. Sonuç olarak tedavi gruplarındaki tüm hastalarda DNA hasarı kontrol grubuna kıyasla daha yüksek bulunmuştur. Hasta grubunda yaş, cinsiyet, ilk veya tekrarlayan tanı, SNRI veya SSRI kullanımı ve fluoksetin kullanımı gibi faktörlerin DNA hasarında anlamlı bir farklılığa neden olmadığı bulunmuştur. Bu çalışma, hem depresyon ve anksiyete hastalığının hemde hastalığın tedavisinin DNA hasarı açısından bir risk faktörü oluşturabileceğini ortaya çıkarmıştır. Bu çalışma, hem depresyon ve anksiyete hastalığının hemde hastalığın tedavisinin DNA hasarı açısından bir risk faktörü oluşturabileceğini ortaya çıkarmıştır.

Keywords

• Depresyon
• anksiyete
• comet testi
• genotoksisite

Determination of possible genotoxic damage by comet assay in patients under treatment due to depression and anxiety disorders

Abstract

Anxiety and depression, very common disorders today, are often seen together. The present study was designed to detect DNA damage in patients diagnosed and treated with depression and anxiety using the Comet test. Thirty-eight patients who received pharmacological therapy (selective serotonin reuptake inhibitors-SSRIs, selective norepinephrine reuptake inhibitors-SNRIs, and antipsychotics) for anxiety and depression and twenty-eight healthy volunteers were included in the study. The comet test was used to evaluate the DNA damage in peripheral blood lymphocytes. As a result, DNA damage in patients in all the treatment groups was higher than those in the control group. It was determined that factors such as age, gender, initial or recurrent diagnosis, SNRI or SSRI use, and fluoxetine use did not cause a statistically significant difference in DNA damage in patient groups. This study revealed that both depression and anxiety disease and the treatment of the disease may present a risk factor for DNA damage. This study revealed that both depression and anxiety and treatment used may have a risk for DNA damage.

Keywords

• Depression
• anxiety
• comet assay
• genotoxicity

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