Türkiye'deki Carlina L. (Asteraceae) cinsine ait taksonların moleküler filogenetik analizi

Abstract

Asteraceae, doğal kimyası, çiçeklenme morfolojisi ve habitata uyumu açısından kozmopolit bir familyadır. Çok sayıda taksonomik ve yeni moleküler filogenetik çalışma olsa da, Asteraceae familyasının ataları tam olarak tanımlanmamıştır. İncelenen çalışma materyali, Carlina L., Asteraceae'nin Cichorioideaea alt familyasına ait Cardueae kabilesinin bir üyesidir. Carlina cinsi, Türkiye Florasında C. lanata, C. vulgaris, C. tragacanthifolia, C. biebersteinii, C. intermedia, C. oligocephala, C. involucrata subsp. libanotica, C. corymbosa, C.graeca ile temsil edilir. Bu çalışmada, Türkiye'de doğal yayılış gösteren Carlina cinsinin moleküler filogenetik analizi yapılmıştır. DNA izolasyonu fenol-kloroform-izoamilalkol kullanılarak yapıldı. Moleküler markör olarak nükleer ribozomal DNA'nın (nrDNA) ITS (Internal Transkripsiyonlu Bölge) ve kloroplast DNA sekanslarının trnL-F (Transfer RNA kodlama) bölgesi kullanıldı. İç grup taksonu olarak Carlina, dış grup taksonu olarak Cardopatium, Atractylis ve Carthamus seçilmiştir. Türkiye'de yayılış gösteren Carlina cinsine ait taksonlar, maksimum parsimoni kriteri ile Branch-and-Bound algoritması kullanılarak filogenetik olarak analiz edilmiştir. 952 en iyi parsimoni ağacı kullanılarak veri seti analizi yapılmıştır. Bir ağaç topolojisi en güvenirlikte ve dallar güçlü bir şekilde Bootstrap desteğine sahiptir.

Keywords

• Asteraceae
• Cardueae
• ITS
• trnL-F
• Filogenetik Analiz

Molecular phylogenetic analysis of the taxa belonging to the genus Carlina L.(Asteraceae) in Turkey

Abstract

Asteraceae, is a cosmopolitan family in terms of natural chemistry, flowering morphology, and adaptation to habitat. Even if there are numerous taxonomic and new molecular phylogenetic studies, the ancestors of the Asteraceae family are not well defined. Study material under consideration, Carlina L., is a member of the tribe Cardueae which belong to Cichorioideaea subfamily of the Asteraceae. The genus Carlina is represented by C. lanata, C. vulgaris, C. tragacanthifolia, C. biebersteinii, C. intermedia, C. oligocephala, C. involucrata subsp. libanotica, C. corymbosa, C.graeca in The Flora of Turkey. In this study, molecular phylogenetic analysis of the genus Carlina, which has a natural distribution in Turkey, has been made. The DNA isolation was performed using phenol- chloroform- isoamylalcohol. ITS (Internal Transcribed Region) of the nuclear ribosomal DNA (nrDNA) and the trnL-F (Transfer RNA coding) region of the chloroplast DNA sequences were used as molecular markers. Carlina was selected as ingroup taxon and Cardopatium, Atractylis and Carthamus were outgroup taxa. Taxa belonging to the genus Carlina distributed in Turkey were analyzed phylogenetically using Branch-and-Bound algorithm with maximum parsimony criterion. Data set analysis using 952 best parsimony trees was made. One tree topology was the most reliable and clads have strongly Bootstrap support.

Keywords

• asteraceae
• cardueae
• ITS
• trnLF
• Phylogenetic analysis

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