Antibiyofilm aktivitenin değerlendirilmesinde kullanılan in vitro-statik testler

Year 2025, Volume: 27 Issue: 2, 762 - 779, 15.07.2025

Simay Aldağ , Ayşenur Yazıcı

https://doi.org/10.25092/baunfbed.1527387

Abstract

Biyofilmler, genellikle farklı mikroorganizma türlerini ve bunların salgıladıkları polisakkaritleri, ekstrasellüler DNA (e-DNA)’yı ve proteinleri içeren yapılardır. Günümüzde insan enfeksiyonlarının %80’inden fazlasının biyofilm kaynaklı olduğu tahmin edilmektedir. Bu nedenle, yeni antibiyofilm ajanların araştırılması ve biyofilm direnç mekanizmalarının anlaşılması oldukça önemlidir. Biyofilm oluşumları birçok faktör tarafından etkilendiği için uygun yöntem seçimi kritik öneme sahiptir. Bu derleme çalışmasının amacı, biyofilm çalışmalarında, antibiyofilm ajanların araştırılmasında ve biyofilmlerin moleküler yapılarının incelenmesinde kullanılan statik yöntemler hakkında bilgi vermektir. Tüm klinik çalışmalara uygun ideal bir biyofilm çalışma yöntemi bulunmamakla birlikte, bu derleme çalışmasında, doğal veya sentetik moleküllerin araştırılmasında kullanılan mevcut in vitro-statik yöntemler özellikleri, avantajları ve dezavantajları ile ele alınmıştır.

Keywords

Biyofilmler , statik yöntemler , biyofilm analiz yöntemleri , antibiyofilm aktivite yöntemleri.

Thanks

Yüksek Teknoloji Uygulama ve Araştırma Merkezi (YUTAM)’da bulunan Taramalı Elektron Mikroskobu (SEM) görüntülerinin alınmasında yardımcı olan Dr. Öğr. Üyesi Mustafa Yazıcı’ya teşekkürlerimizi sunarız. Diğer fotoğraflar, YUTAM, Moleküler Mikrobiyoloji laboratuvarında çekilmiştir.

In vitro-static tests used to evaluate antibiofilm activity

Abstract

Biofilms are structures that usually contain different species of microorganisms and secreted polysaccharides, extracellular DNA (e-DNA) and proteins. It is currently estimated that over 80% of human infections involve biofilm-associated microorganisms. Therefore, it is very important to discover new antibiofilm agents and understand biofilm resistance mechanisms. Given that biofilm formation is influenced by various factors, selecting an appropriate method is essential for such studies. The aim of this review is to provide information about methods used in biofilm studies, the search for antibiofilm agents, and examinations of the molecular structures of biofilms. Although there is no ideal biofilm study method suitable for all clinical applications, this review presents the features, advantages, and disadvantages of current in vitro static methods used to evaluate natural or synthetic compounds.

Keywords

Biofilms , static methods , biofilm analysis methods , antibiofilm activity methods.

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