Investigation of paraoxonase1 enzyme activity and Q/R 192 polymorphism in severe COVID-19 cases

Abstract

COVID-19, an infection associated with numerous cases and deaths globally since 2019, has been a subject of extensive research. Paraoxonase1 (PON1), recognized for its antioxidant activity and contribution to the reduction of oxidative damage, is the focus of this study. The research aims to measure the activity of the human serum PON1 enzyme with detoxification and antioxidant properties in COVID-19 cases leading to pneumonia. Additionally, the phenotype of the PON1 Q/R 192 polymorphism is investigated and compared with a control group. The study includes 26 severe COVID-19 cases and 24 healthy individuals. The phenotype distribution of patients is determined as 38.48% QQ, 53.83% QR, and 7.69% RR, while in the control group, these percentages are 50.00% QQ, 29.17% QR, and 20.83% RR. Furthermore, PON1 activity is measured in 26 COVID-19 patients and 24 healthy individuals. The results indicate distinct differences in the phenotype distribution of the PON1 Q/R 192 polymorphism between COVID-19 patients and the control group. The percentages of QQ, QR, and RR are unique in both groups. Additionally, PON1 activity is measured, revealing that the control group exhibits higher PON1 activity compared to the affected individuals.

Keywords

• Paraoxonase1
• SARS-COV-2
• COVID-19
• Q/R 192R polymorphism

Ağır COVID-19 geçiren hastalarda paraoksonaz1 enzim aktivitesinin ve Q/R 192 polimorfizminin araştırılması

Abstract

COVID-19, 2019 yılından bu yana dünya genelinde çok sayıda vaka ve ölümle ilişkilendirilen bir enfeksiyon olup, kapsamlı araştırmalara konu olmuştur. Antioksidan aktivitesi ve oksidatif hasarın azaltılmasına katkısı ile bilinen Paraoksonaz1 (PON1), bu çalışmanın odak noktasını oluşturmaktadır. Araştırma, zatürreye yol açan COVID-19 vakalarında detoksifikasyon ve antioksidan özelliklere sahip insan serum PON1 enziminin aktivitesini ölçmeyi amaçlamaktadır. Ayrıca, PON1 Q/R 192 polimorfizminin fenotipi incelenmiş ve bir kontrol grubu ile karşılaştırılmıştır. Çalışma, 26 ağır COVID-19 vakası ve 24 sağlıklı bireyi içermektedir. Hastaların fenotip dağılımı %38,48 QQ, %53,83 QR ve %7,69 RR olarak belirlenirken, kontrol grubunda bu oranlar sırasıyla %50,00 QQ, %29,17 QR ve %20,83 RR olarak bulunmuştur. Bunun yanı sıra, 26 COVID-19 hastası ve 24 sağlıklı bireyde PON1 aktivitesi ölçülmüştür. Sonuçlar, COVID-19 hastaları ile kontrol grubu arasında PON1 Q/R 192 polimorfizminin fenotip dağılımında belirgin farklılıklar olduğunu göstermektedir. QQ, QR ve RR yüzdeleri her iki grupta da benzersizdir. Ayrıca, PON1 aktivitesi ölçümleri, kontrol grubunun etkilenen bireylere kıyasla daha yüksek PON1 aktivitesi sergilediğini ortaya koymaktadır.

Keywords

• Paraoksonaz1
• SARS-COV-2
• COVID-19
• Q/R 192R polimorfizim

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