The effect of lead and mercury heavy metal ions on body weight and serum alkaline phosphatase level in albino mice

Abstract

Seventy albino mice were used as material in this study. The mice divided into three groups as control , treatment 1 and treatment 2. The control group were fed with normal food (food and water), treatment 1 group were fed with food containing Pb with different dosages (food and aqueus solution containing Pb), and treatment 2 group were fed with food containing Hg in different dosages (food and aqueus solution containing Hg). The experimental period lasted 15 days. At the end of the experimantal period initially mice were weighed and then alkaline phosphatase (ALP) level were determined in blood taken during the slaughtering using autoanalyzer. The body weight of mice were decreased with increasing metal ion concentration in treatment. It was observed that serum alkaline phosphatase level was significantly decreased (p<0.05) with increasing Pb and Hg concentration

Keywords

• Albino mice, lead, mercury, alkaline phosphatase

Kurşun ve civa ağır metal iyonlarının albino farelerde canlı ağırlık ve serum alkalen fosfataz düzeyi üzerine etkisi

Abstract

Bu çalışmada 70 adet albino fare materyal olarak kullanıldı. Kontrol, deneme 1 ve deneme 2 olmak üzere fareler 3 gruba ayrıldı. Kontrol grubu normal besin (yem ve su), ikinci grup farklı dozlarda kurşun (Pb) içeren besin (yem ve kurşun içeren sulu çözelti), üçüncü grup ise farklı dozlarda civa (Hg) içeren besin (yem ve civa içeren sulu çözelti) ile beslendi. Deneme süresi 15 gün olarak belirlendi. Deneme süresi bitiminde fareler ilk olarak tartıldı ve sonrasında kesim esnasında alınan kan örneklerinde Alkalen fosfataz (ALP) enzim düzeyi otoanalizör kullanılarak belirlendi. Farelerde uygulanan metal iyon dozunun artması ile birlikte canlı ağırlık değerlerinde önemli azalmalar belirlendi. Pb ve Hg uygulama dozu ile birlikte serum alkalen fosfataz düzeylerinde belirgin azalma olduğu gözlendi (p

Keywords

• Albino fare, kurşun, civa, alkalen fosfataz

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