Kitosan temelli hidrojellerin hazırlanması ve 5-Florourasil salımı davranışlarının incelenmesi

Abstract

Kitosan (CH), poli(2-akrilamido-2-metilpropansülfonik asit) (pAMPS) ve poliakrilamid (pAAm) hidrojelleri içeren, üçlü, iç içe geçmiş ağ yapılı hidrojeller (IPNHs) ve sadece kitosan hidrojelleri sentezlendi ve model ilaç olarak seçilen 5-Florourasil (5-FLU) biyoetken moleküllerinin denetimli salımı için araştırıldı. IPNHs; çapraz bağlayıcı olarak seçilen glutaraldehit (GLU) ve N,N’-metilenbisakrilamid (MBA) varlığında monomerlerin radikalik polimerleşmesi ile hazırlandı. Hidrojellerin spektroskopik analizleri; Fourier dönüşümlü infrared spektroskopisi ile yapıldı. IPNH’lerin kinetik ve dinamik şişme çalışmaları 37 ◦ C’de; mide sıvısına (pH=1.1) ve ince bağırsak sıvısına (pH=7.4) benzetilmiş tampon çözeltilerde gastrointestinal ilaç salımı çalışmaları için in vitro olarak araştırıldı. Şişme ve ilaç salım deneysel verileri; şişme ve salım proseslerinin ikinci dereceden kinetiğe uyduğunu gösterdi. Tutuklanan biyoetken türlerin IPNH’lerden ve kitosan hidrojellerinden salımının; hidrojel bileşimi ve ortamın pH’sına bağlı olduğu anlaşıldı. Sonuç olarak; kitosan temelli IPNH’lerin; oral gastrointestinal salım sistemlerindeki formülasyonlar için ümit verici adaylar olabileceği söylenebilir. 

Keywords

• Hidrojel,kitosan,akıllı jeller,denetimli salım,5-Florourasil

Preparation of the hydrogels based chitosan and investigation of release behaviours of 5-Fluorouracil

Abstract

Ternary interpenetrating polymeric networks hydrogels (IPNHs) containing chitosan (CH), poly(2-acrylamido-2-methylpropanesulfonic acid) (PAMPS) and polyacrylamide (pAAm) polymers and bare chitosan hydrogels were synthesized and investigated for controlled release of bioactive molecules utilizing a model drug, 5-Fluorouracil (5- FLU). IPNHs were prepared by radical polymerization of monomers in presence of glutaraldehyde (GLU) and N,N’-methylenebisacrylamide (MBA) selected as crosslinkers. Spectroscopic analyses of these hydrogels were made with Fourier transform infrared spectroscopy. Kinetic and dynamic swelling studies of IPNHs were carried out in buffer solutions simulated gastric fluid (pH=1.1) and simulated intestinal fluid (pH=7.4) at 37 ◦ C for studies of gastrointestinal drug delivery as in vitro. The experimental data of swelling and drug release studies suggest clearly that the swelling and release processes obey second-order kinetics. It was realized that the release of the entrapped bioactive species from IPNHs and chitosan hydrogels depends on the composition of the hydrogel and pH of the medium. As a result, it can be said that IPNHs based chitosan could be promising candidates for formulations in oral gastrointestinal delivery systems

Keywords

• Hydrogel,chitosan,intelligent gels,controlled release,5-Fluorouracil

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