Simmental ırkı sığırlardan elde edilen sütlerde antioksidan aktivitelerin belirlenmesi

Abstract

Son yıllarda sağlık üzerine olumlu etkileri olan biyoaktif bileşenleri içeren gıdalara olan talep giderek artmaktadır .  Süt bu gıda maddeleri arasındadır.  Süt, hastalık risklerini azaltıcı ya da önleyici nitelikte antioksidan bileşenlerini de yapısında bulundurmaktadır.  Proteinler, vitaminler (E ve C), retinol, beta karoten, glutatyon (GSH), katalaz (CAT), süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH-Px) gibi birçok biyoaktif bileşen sütün antioksidan aktivitesini oluşturmaktadır.  Bu çalışmada Tunceli ilinde satılan sütlerin GSH düzeyi ile GSH-Px, SOD ve CAT aktivitelerinin araştırılması amaçlanmıştır.  Araştırma örnekleri Tunceli ili merkez ilçesinde satılan sütlerden elde edilmiştir. Elde edilen süt parametrelerine ait veriler, süt sığırları için rapor edilen seviyeler ile tutarlı bulunmuştur.

Keywords

• Süt,antioksidan,glutatyon,glutatyon peroksidaz,katalaz

Determination of antioxidant activities in milks obtained from simmental breed of cattle

Abstract

In recent years there has been an increasing demand for foods containing bioactive compounds that have positive effects on health.  Milk is among these foodstuffs.  Milk also contains antioxidant compounds that reduce or prevent the risk of various diseases.  Many bioactive compounds such as proteins, vitamins (E and C), retinol, beta carotene, glutathione (GSH), catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) form antioxidant activity in milk.  In this study, it was aimed to investigate the GSH level, GSH-Px, SOD and CAT activities in milk which was obtained from Simmental breed of cattle and raised in Tunceli province.  For this purpose, milk samples were obtained from the enterprises producing milk of simmental breed of cattle at the central district of Tunceli province.  The data on the milk parameters we obtained were found to be consistent with the levels reported for dairy cattle.

Keywords

• Milk,antioxidant,glutathione,glutathione peroxidase,catalase

References

1. Anonim, “What is optimum nutrition?”, The Institute For Optimum Nutrition, (2005). http://www.ion.ac.uk/optimum.htm, (13.11.2015).
2. Kris-Etherson, P.M., Hecker, K.D., Bonanome, A., Coval, S.M., Binkoski, A.E., Hilpert, K.F., Griel, A.E., and Etherton, T.D., Bioactive compounds in foods: Their role in the prevention of cardiovascular disease and cancer. American Journal of Medicine, 113, 71-83, (2002).
3. Nayir, M.S., Sütün yoğurda dönüşümü sırasında içerdiği fenolik antioksidan maddelere prebiyotik bakteri etkisinin incelenmesi, Yüksek Lisans Tezi, Trakya Üniversitesi, Fen Bilimleri Enstitüsü, Edirne (2008).
4. Virtanen, T., Pihlanto, A., Akkanen, S. and Korhonen, H., Development of antioxidant activity in milk whey during fermentation with lactic acid bacteria, Journal of Applied Microbiology, 102, 1, 106-115, (2006).
5. Lin, M.Y. and Yen, C.L., Antioxidative ability of lactic acid bacteria, Journal of Agricultural and Food Chemistry, 47, 1460-1466 (1999).
6. Lindmark-Månsson, H. and Åkesson, B., Antioxidative factors in milk. British Journal of Nutrition, 84, 103-110, (2000).
7. Nielsen, J.H., Hald, G., Kjeldsen, L., Andersen, H.J. and Ostdal, H., Oxidation of ascorbate in raw milk induced by enzymes and transition metals, Journal of Agricultural and Food Chemistry, 49, 2998–3003, (2001).
8. Pihlanto, A., Antioxidative peptides derived from milk proteins. International Dairy Journal, 16, 1306 –1314, (2006).

9. Öğünç, A.V. and Yalçın, A.S., Süt serumu proteinlerinin in vitro koşullardaki antioksidan etkileri. Marmara Eczacılık Dergisi, Vol.15: 18-24. (2011).
10. Akkuş, İ., Serbest radikaller ve fizyopatolojik etkileri. Mimoza Yayınları. 1: 3-95. Konya.(1995).
11. Madi, B.C., Sandal, J. and Bannet, R., Effects of female relative in labor: A randomized controlled trial, Birth, 26, 1, 4–8 (1999).
12. Mitchel, J.B. and Russo, A., The role of glutathione in radiation and drug induced cytotoxicity, British Journal of Cancer, 55, 96-104, (1987).
13. Compoti, M., Glutathione depleting agents and lipid peroxidation in the aging rat, Comparative Biochemistry and Physiology, 88, 177- 180, (1987).
14. Ziegler, D.M., Role of reversible oxidation-reduction of enzyme thiols-disulfides in metabolic regulation, Annual Review of Biochemistry, 54, 305-2229, (1985).
15. Lee, J., Koo, N. and Min, D.B., Reactive oxygen species, aging, and antioxidative nutraceuticals, Compherensive Reviews in Food Sience and Technology, 3, 21-33 (2004).
16. Velioğlu, S., 2000. Doğal antioksidanların insan sağlığına etkileri, Gıda, 25, 3, 167-176, (2000).
17. Matés, J.M., Pérez-Gómez, C. and de Castro, N.I.,1999. Antioxidant ezymes and human diseases, Clinical Biochemistry, 32, 8, 595-603 (1999).
18. Yüzügüllü, Y. and Ögel, B.Z., Çift aktiviteli katalaz-fenol oksidazın ve diğer katalazların gıda sanayisindeki önemi, Gıda, 38, 2, 111-118 (2013).
19. Chen, J., Lindmark-Mainsson, H. and Akesson, B., Optimisation of a coupled enzymatic assay of glutathione peroxidase activity in bovine milk and whey, International Dairy Journal, 10, 347-351 (2000).
20. Yining, B., Wenbo, Z., Zheng, Z. and Wang, Z., Antioxidant Property in vivo of Cheese Whey Enzymatic Hydrolysates. The 5th International Conference on Bioinformatics and Biomedical Engineering. p:1-4 May 2011, Wuhan, China (2011).
21. Beutler, E., Duron, O. and Kelly, B.M., Improved method for the determination of blood glutathione, Journal of Laboratory and Clinical Medicine, 61, 882-888, (1963).
22. Beutler, E., Red cell metabolism. In, Beutler E (Editor). A manual of biochemical methods. New York: Grunef and Strottan, 67-69 (1975).
23. Sun, Y., Oberley, L.W. and Li, Y., A simple method for clinical assay of superoxide dismutase, Clinical Chemistry, 34, 497-500 (1988). Aebi, H., Catalase in vitro, Method Enzymol, 105, 121-126 (1984).
24. Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R.J., Protein measurements with the folin phenol reagent, The Journal of Biological Chemistry, 193, 265-275 (1951).
25. Çimen, M., Fen ve Sağlık Bilimleri Alanlarında Spss Uygulamalı Veri Analizi. Palme Yayıncılık, Yayın No: 905, ISBN: 978-605-355-366-3. Sıhhıye, Ankara (2015).
26. Hoolbrook, J.J. and Hicks, C.L., Variation of superoxide dismutase in bovine milk, Journal of Dairy Science, 61, 1072-1077 (1978).
27. Kankare, V. and Antila, V., The effect of xanthine oxidase and superoxide dismutase as well as cell counts on the oxidation of fat in bovine milk, Finnish Journal of Dairy Science, 2, 32-40 (1982).
28. Fox, F.P., Exogenous enzymes in dairy technology. University Colage. 17, 3, 173-199. Cock, Ireland, (2003).
29. Prasad, T. and Kundu, M.S., Serum IgG and IgM responses to sheep red blood cells (SRBC) in weaned calves fedmilk supplemented with Zn and Cu, Nutrition, 11, 712–15, (1995).
30. Gutteridge, J.M.C. and Halliwell, B., Antioxidant in nutrition, health and disease. Oxford University Press: New York (1994).
31. Urso, M.L. and Clarkson, P.M., Oxidative stress, exercise, and antioxidant supplementation, Toxicology, 189, 41 -54 (2003).
32. Yu, B.P., Cellular defenses against damage from reactive oxygen species, Physiol Rev, 74, 1, 139-62 (1994).
33. Zima, T., Crkovska, J., Merta, M., Stipek, S., Nemecek, K. and Tesar, V., Activity of the antioxidant.enzymes, glutathione peroxidase, on autosomal dominant 54 polycyctic kidney disease patient, Biochemical Molecular Biology International, 35, 4, 699-704 (1995).
34. Debski, B., Piccano, M.F. and Milner. J.A., Selenium content and distribution of human, cow and goat milk. Journal of Nutrition, 117, 1091-1097, (1987).
35. Koneko, J.J. and Cornelius, C.E., Clinical Biochemistry of Domestic Animals, 3rd ed. Academic Press, New York. 41−376 (1980).