Baklagil bitkisi Indigofera zollingeriana’nın depolanmış tohumların in vitro koşullarda çimlendirilmesi ve geliştirilmesi

Abstract

Deneysel çalışmalar, Endonezya’da zollinger mavisi ya da Indigofera zollingeriana olarak bilinen yem bitkisinin üretimini büyük ölçüde engelleyen faktörün yüksek tohum dormansisi olduğunu göstermektedir.  Bu çalışmada farklı ön muameleler ile yüksek tohum dormansisinin kırılması ve in vitro koşullar altında tohum çimlendirilmesinin  geliştirilmesi amaçlanmıştır. Yapılan denemelerde hem mekanik hem de kimyasal muamelesinin birlikte uygulanması sonrası 0.11 mg/L GA3 uygulamasının tohum çimlenmesinde önemli etkisi olduğu sonucuna varılmıştır.  Çimlenen tohumlar MS besin  ortamına alınarak büyümeye bırakılmıştır.  Bu şekilde çimlendirilen tohumlar sadece  zımpara ya da asit muamelesi uygulaması ile çimlendirilen tohumlara göre daha iyi çimlenme göstermiştir.  Bununla birlikte asit uygulama süresinin artması özellikle çimlenen tohumların köklerinde olumsuz etkiye sebep olmuştur.

Keywords

• em,gibberellik asit,baklagil,mekanik aşındırma,kimyasal aşındırma

Improving in vitro seed sprouting on legume of Indigofera zollingeriana stored seed

Abstract

Experimental evidence shows that notable Indonesian forage crop zollinger blue or Indigofera zollingeriana has high seed dormancy that hinders its reproduction on large scale.  This study reports different pre-treatments to break seed dormancy and improve seed germination of I. zollingeriana seeds under in vitro conditions.  Experimental evidence suggest that both mechanical and chemical scarification followed by treatment with constantly agitated liquid 0.11 mg/L GA3 has significant effects on seed germination of the plant. The germinated seeds were cultured on MS medium to aid seedling growth.  The results showed improved germination and raising of the seedlings compared to the treatments; when the seedlings were germinated using sand paper or acid scarified seeds singly. However, acid scarification for longer time affect negatively on germination especially roots.

Keywords

• Forage,gibberelic acid,legumes,mechanical scarification,chemical scarification

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