Neonikotinoid insektisit thiamethoxamın Oreochromis niloticus’ta oksidatif stres parametreleri üzerine etkisi

Yıl 2018, Cilt: 20 Sayı: 2, 224 - 234, 01.12.2018

Özgür Fırat , Tüzin Aytekin

https://doi.org/10.25092/baunfbed.427757

Cited By: 4

Öz

Araştırmamızda neonikotinoid insektisit olan thiamethoxamın toksik etkileri, Nil tilapyası Oreochromis niloticus’ta oksidatif stres parametreleri ile araştırılmıştır. Bu amaçla balıklar 4 ve 14 günlük sürelerle 60 ve 120 mg/L thiamethoxam etkisine bırakılmış ve solungaç ve bağırsak dokularındaki katalaz (CAT) ve süperoksit dismutaz (SOD) aktiviteleri ile glutatyon (GSH) ve malondialdehit (MDA) düzeyleri belirlenmiştir. Thiamethoxamın etkisinde özellikle de yüksek ortam derişiminde CAT aktivitesinde solungaç ve bağırsak dokularında 4 günlük süre sonunda anlamlı bir artış; 14 günlük süre sonunda ise solungaç dokusunda anlamlı bir azalış belirlenmiştir (P˂0.05). SOD aktivitesi solungaçta her iki etki süresi sonunda insektisidin düşük ve yüksek ortam derişimlerinde; bağırsakta ise 14 günlük süre sonunda ve yüksek ortam derişiminde artış göstermiştir (P˂0.05). GSH düzeyi özellikle de 120 mg/L thiametoxam etkisinde her iki dokuda da 4 günlük süre sonunda anlamlı bir artış; 14 günlük süre sonunda ise önemli bir azalış göstermiştir (P˂0.05). Solungaç ve bağırsak dokularındaki MDA düzeyi ise, 14 günlük süre sonunda thiametoxamın yüksek ortam derişiminde önemli düzeyde artmıştır (P˂0.05). Sonuç olarak çalışmamız, thiametoxamın özellikle de yüksek ortam derşiminde O. niloticus’ta oksidatif strese neden olduğunu göstermektedir. 

Anahtar Kelimeler

Oreochromis niloticus, pestisit, thiamethoxam, antioksidan parametreler, malondialdehit

Effect of neonicotinoid insectiside thiamethoxam on oxidative stress parameters in Oreochromis niloticus

Öz

In our study, toxic effects of thiamethoxam, a neonicotinoid insectiside, were investigated by oxidative stress parameters in Nile tilapia, Oreochromis niloticus. For this purpose fish were exposed to 60 and 120 mg/L thiamethoxam for 4 and 14 days and activities of catalase (CAT) and superoxide dismutase (SOD) and levels of glutathione (GSH) and malondialdehyde (MDA) in the gill and intestine were determined. In exposure of thiametoxam especially in its higher medium concentration, it was determined a significant increase in CAT activities of gill and intestine tissues after 4 days and a significant decrease in its activity of gill after 14 days (P˂0.05). SOD activity elevated in gill in all the tested concentrations of thiamethoxam after both exposure periods and in intestine in its higher concentration at 14 days (P<0.05). In both gill and intestine tissues, especially in higher medium concentration of thiamethoxam GSH levels indicated significantly an increase after 4 days and a decline after 14 days (P˂0.05). MDA levels in the both tissues significantly elevated in exposed to higher concentration of thiamethoxam at the end of the exposure period (P<0.05). In conclusion the present research showed that exposure of thiamethoxam, especially its higher concentration, caused oxidative stress in O. niloticus.

Anahtar Kelimeler

Oreochromis niloticus, pesticide, thiamethoxam, antioxidant parameters, malondialdehyde

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