Hedef Gen Çıkarımı İçin Makine Öğrenmesi Tabanlı Mikrodizi Verilerinin Analizı

Abstract

Mikrodizi teknolojisi gen ifadesindeki farklılıkların tespit edilmesinde kullanılır. Bu teknoloji ilaç geliştirme süreçlerinden tedavi süreçlerinin iyileştirilmesine birçok alanda katkı sağlamaktadır. Bu çalışmada, kronik hipoksi tedavisinin fare beyni üzerindeki etkisi ve oksidatif strese maruz kalan fare nöronlarının gen üzerindeki etkisi ile ilgili veri kümeleri üzerinde mikrodizi analizi yapılmıştır. Çalışmada açık erişim sağlanabilen iki farklı mikrodizi veri kümesi kullanılmıştır ve makine öğrenmesi yöntemleri ile çalışılmıştır. İlk adım olarak veri kümelerinin indirilmesi, ardından ön işleme tabi tutularak normalizasyon uygulanması sağlanmıştır. Bu aşamalar ile veri kümeleri gen çıkarımı için çalışmaya uygun hale getirilmiştir. Hazırlanan bu gen ekspresyon ifadeleri üzerinden istatistiksel ve makine öğrenmesi yöntemleri kullanılarak analizler gerçekleştirilmiş ve hedef gen çıkarımı sağlanmıştır.

Keywords

• Mikrodizi
• makine öğrenmesi
• hedef gen çıkarımı
• gen ekspresyon analizi

Machine Learning Based Microarray Data Analysis For Target Gene Inference

Abstract

Microarray technology is used to detect differences in gene expression. This technology contributes to many fields, from drug development processes to the improvement of treatment procedures. In this study, microarray analysis was performed on datasets related to the effect of chronic hypoxia treatment on the mouse brain and the effect of oxidative stress on the genes of mouse neurons. Two different publicly accessible microarray datasets were used in the study, and machine learning methods were employed. The first step involved downloading the datasets, followed by preprocessing and normalization. These steps made the datasets suitable for gene extraction. Statistical and machine learning methods were applied to the prepared gene expression profiles, leading to the inference of target genes.

Keywords

• Microarray
• machine learning
• target gene inference

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