Heat Shock Protein 70 extraction from human tooth for ELISA

Abstract

Abstract

Protein extraction from bone is extremely difficult task because the bone tissue is hard to dissolve. Special care must be taken during extraction to prevent protein degradation and/or unfolding. Boiling is a traditional method for extracting protein from bones, but heat denaturation during this process destroys tertiary structure of some proteins irreversibly.  Dental sciences employ tooth based research and extraction of proteins or other macromolecules from tooth are required most of the time. A new method was developed in our laboratory to extract proteins from tooth. This method overcomes heat or chemical denaturation of proteins and requires couple days. Although the method extract relatively less protein, the total amount of protein is sufficient for ELISA and proteomics.

Keywords: Heat Shock Protein 70, tooth, ELISA

Özet

ELISA için insan dişinden ısı şok protein 70 ekstraksyonu

Kemikten protein ekstraksyonu kemik dokusunun çözülmesi oldukça kısıtlı olduğundan zor bir işlemdir. Ekstraksyon sırasında protein yıkımı ve/veya katlanmasını engellemek için özel önlem alınmalıdır. Kaynatmak kemiklerden protein çıkartmak için geleneksel bir yöntemdir fakat bu işlem süresince ısı kaynaklı denatürasyon bazı proteinleri geri dönüşümsüz üçüncül yapılarını bozarlar. Diş bilimleri çoğu kez diş ile ilgili çalışmalar,  dişten protein veya diğer moleküllerin ekstraksiyonunu gerektirir. Laboratuarımızda dişten protein ekstraksyonu için yeni bir metot geliştirildi.  Bu metot ısı ve kimyasal denaturasyonun önüne geçer ve bir kaç gün gerektirir. Yeni metot göreceli az protein ekstrakte etsede, elde edilen toplam protein ELISA ve proteomik çalışmaları için yeterlidir.

Anahtar Sözcükler: Isı şok protein 70, diş, ELISA

Keywords

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