Association between serum leptin level and osteoporosis in patients with type 2 diabetes mellitus

Abstract

Abstract

Aim: As osteoporosis and diabetes mellitus are widespread diseases, it is an important question whether leptin plays a role in bone physiology or not. In this study, we aimed to investigate the relationship between serum leptin level, diabetes and osteoporosis. Methods: The present study was conducted on 49 healthy individuals and 77 patients with type 2 Diabetes mellitus (DM), a total of 126 individuals, between 40 and 60 of ages. Between 08-10 hours in the morning, venous blood samples of 15 mL were drawn from forearm veins of the individuals and transferred into concerned test tubes. After blood samples were taken, Bioelectrical Impedance Analysis (BIA) was performed. Bone mineral density (BMD) was measured by DEXA (Dual energy X-ray absorptiometry) at L_1-4 (anterior-posterior) and femur. Student t test and correlation analysis were used for the statistical analysis in the study. Results: Leptin level of women in both groups were significantly higher than those of men in both groups (p<0.05). BMD values of men in patient group was significantly lower compared to those of men in control group (p<0.05). Similarly, BMD values of women in patient group was significantly lower compared to those of women in control group (p<0.05). A positive correlation was found between femur neck bone mineral density and leptin of men in both groups (p<0.05). Conclusions: In terms of bone mineral density, femur neck measurements of women were more sensitive than L_1-L4 measurements. Finding a positive correlation between femur neck bone mineral density and leptin levels in all men and having no such correlation in women may be related to more effective function of leptin hormone in men.

Keywords: Leptin, osteoporosis, type 2 diabetes mellitus

Özet

Tip 2 diabetes mellitus hastalarında leptin düzeyi ile osteoporoz arasındaki ilişki

Amaç: Osteoporoz ve diyabet çok yaygın hastalıklar olduğu için, leptinin insan kemik fizyolojisinde herhangi bir rolü olup olmadığı önemli bir sorudur. Bu çalışmada, serum leptin konsantrasyonu ile diyabet ve osteoporoz arasındaki ilişkinin araştırılmasını amaçladık. Yöntem: Çalışma yaşları 40 ile 60 arasında değişen, tip 2 diabetes mellituslu (DM) 77 hasta ve 49 sağlıklı olmak üzere toplam 126 birey üzerinde yapılmıştır. Sabah saat 08:00-10:00 arası bireylerin ön kol venasından girilip yaklaşık 15 mL venöz kan örneği alınarak yapılacak testlerle ilgili deney tüplerine aktarıldı. Kan örnekleri alındıktan sonra tüm olgulara Biyoelektriksel İmpedans Analizi (BİA) uygulandı. Kemik mineral yoğunluğu (KMY) ise DEXA (Dual enerji X-ray absorptiometri) yöntemi ile L_1-4 (ön-arka) ve femurdan ölçüldü. Çalışmanın istatistiksel analizinde student t testi ve korelasyon analizi kullanıldı. Bulgular: Kontrol ve hasta gruplarında kadınların leptin düzeylerinde görülen artış, erkeklerinkine göre anlamlı bulunmuştur (p<0.05). Hasta erkek grubu kontrol grubu ile karşılaştırıldığında, KMY değeri anlamlı olarak düşük bulunmuştur (p<0.05). Kadın hasta grubundaki KMY değerinde azalma kontrol gurubuna göre anlamlıydı (p<0.05). Tüm erkeklerde femur boynu KMY değerleri ile leptin arasında pozitif bir korelasyon bulunmuştur (p<0.05). Sonuçlar: Kadınlar KMY'ye göre değerlendirildiğinde femur boynu ölçümleri, L_1-L4 ile karşılaştırıldığında daha hassasiyet göstermektedir. Tüm erkeklerde femur boynu KMY değerleri ile leptin arasında pozitif bir korelasyon bulunurken kadınlarda bulunmaması erkeklerde leptin hormonunun daha etkili olmasından kaynaklanabilir.

Anahtar sözcükler: Leptin, osteoporoz, tip 2 diabetes mellitus

Keywords

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