İvesi koyun ırkında FecXI mutasyonunun PCR-RFLP yöntemi ile araştırılması

Abstract

Koyunlarda çoklu doğumu etkileyen mutasyonların tanımlanması genetik ıslah çalışmalarının etkinliği açısından oldukça önemlidir. Markör destekli seleksiyon (MAS) ile ıslah çalışmalarının yapılabilmesi için öncelikli olarak majör veya olası yeni mutasyonların tanımlanması ve ırk düzeyinde etkisinin ortaya konması gerekmektedir. Bu çalışmada İvesi koyun ırkında FecXI (Inverdale) mutasyonunun PCR-RFLP yöntemi ile araştırılması amaçlanmıştır. Çalışmanın materyalini, Adıyaman ilinde saf yetiştiriciliği yapılan bir işletmeden alınan 50 baş İvesi koyun ırkına ait kan örnekleri oluşturmuştur. Kandan genomik DNA’nın elde edilmesinde ticari izolasyon kiti kullanılmıştır. Mutasyonun bulunduğu BMP15 geninin 154 baz çiftlik bölgesi PCR ile çoğaltılmış ve sonrasında tüm PCR ürünlerinde FecXI mutasyonu PCR-RFLP yöntemi ile araştırılmıştır. PCR-RFLP sonuçları, incelenen İvesi koyunlarının FecXI mutasyonunu taşımadığını göstermiştir. İvesi koyun ırkında çoklu doğum ile ilişkili majör ve tanımlanan yeni mutasyonların farklı moleküler yöntemler ile araştırılması önerilmektedir.

Keywords

• Koyun
• Çoklu doğum
• İvesi
• PCR-RFLP
• FecXI
• BMP15

investigation of FecXI mutation by PCR-RFLP method in Awassi sheep breed

Abstract

The identification of mutations affecting multiple births in sheep is critical for the efficacy of genetic breeding studies. Before carrying out breeding studies with marker-assisted selection (MAS), it is necessary to identify major or potential novel mutations and determine their effects at the level of the breed. The aim of this study was to investigate the FecXI (Inverdale) mutation in the Awassi sheep breed using the PCR-RFLP technique. The study used blood samples from 50 ewes collected from a pure breeding farm in the Adiyaman province of Turkey. To isolate genomic DNA from blood, a commercial isolation kit was utilized. The 154 base pair area of the BMP15 gene where the mutation was located was amplified by PCR, and the FecX mutation was analyzed in all PCR products using the PCR-RFLP technique. The FecXI mutation was not found in the Awassi sheep tested using PCR-RFLP. It is recommended that the major and recently found novel mutations with multiple births be investigated by different molecular methods in the Awassi sheep breed.

Keywords

• sheep
• multiple births
• Awassi
• PCR-RFLP
• FecXI
• BMP15

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