Şap Hastalığının Miyokardiyal Formundan Ölen İki Kuzuda Kalp ve Dilde Gözlenen Patolojik Bulgular

Year 2017, Volume: 6 Issue: 2, 15 - 22, 08.01.2018

Mustafa Kemal Çiftçi Muammer İnanç

Abstract

Şap hastalığının (FMD) morbiditesinin çok yüksek olması nedeniyle salgınların önüne geçilebilmesi ve kayıpların azaltılmasında hızlı teşhis, özellikle hastalığın ilk çıkışında son derece önemlidir. Bu nedenle çalışmada, şap hastalığının miyokarditis formu sonucu ölen 2 adet kuzuda kalp ve hastalığın teşhisine katkı sağlaması amacıyla makroskobik bir lezyon olmamasına rağmen dilden de örnekler alınmış ve histopatolojik metotlarla incelenmiştir.

Bu maksatla Şap Enstitüsü (Türkiye) tarafından FMDV serotip A Nepal-84 suşu enfeksiyonundan öldüğü belirlenen 2 adet kuzuda gözlenen makroskobik bulgular kaydedilerek, histopatolojik ve immunohistokimyasal (IHC) yöntemlerle incelendi. Kalp kasında makroskobik ve mikroskobik olarak şap hastalığına işaret patolojik lezyonlar gözlendi. IHC boyamalarda da makrofajlar ve miyositlerde FMDV serotip A Nepal-84 (G-VII) yönünden antijen pozitiflik belirlendi. Dilde ise histopatolojik olarak stratum spinozum hücrelerinde vezikül oluşumunun başlangıcına işaret eden belirgin bir hidropik dejenerasyon gözlendi. Bu bulgular şap hastalığının histopatolojik teşhisinde miyokarditis bulgularına önemli derecede destek sağlayacak bulgulardır. Böylece miyokarditis formuna ilgili ölümlerde her ne kadar makroskobik düzeyde vezikül oluşumu gözlenmiyorsa da histopatolojik incelemeler için dilden de örnek alınmasının teşhis için katkılı olacağı anlaşılmıştır.

Keywords

Histopatoloji, immunohistokimya, şap hastalığı

The Pathologic Findings in Heart and Tongue in Two Lambs Died from Myocardial Form of Foot and Mouth Disease

Abstract

Rapid diagnosis is crucial to prevent and control outbreaks, especially at the first appearance of the disease, due to the high morbidity of foot-and-mouth disease (FMD). In this study, samples from two lambs which were determined to have died due to myocarditis form of FMD, and to contribute to the diagnosis of the disease their tongues without macroscopic lesions were investigated by histopathological methods.

For this purpose, macroscopic findings were recorded from two lambs which were determined to have died due to FMDV serotype A Nepal-84 strain infection by the Şap Institute (Turkey). Also, samples from two lambs were examined by histological and immunohistochemical (IHC) methods. Macroscopic and microscopic lesions that signs of foot-and-mouth disease were observed in the heart. By IHC staining, macrophages and monocytes were evaluated as antigen positive for FMDV serotype Nepal-84 (G-VII). Histopathologically, significant hydropic degeneration was observed in the stratum spinosum cells of tongue indicating the onset of vesicle formation. These findings are important findings in the histopathological diagnosis of FMD that will provide important support for myocarditis findings. Thus, although vesicle formation is not observed at the macroscopic level in the deaths related to the myocarditis form, it has been understood that taking the sample for the histopathological examinations will contribute to the diagnosis.

Keywords

Histopathology, immunohistochemistry, foot and mouth disease

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