Pollen Micromorphology of 12 Taxa of Delphinium in Türkiye and Their Taxonomic Importance

Birol Başer; Süleyman Aksu; Murat Kürşat; Ozan Bekmezci

doi:10.17678/beuscitech.1787364

Pollen Micromorphology of 12 Taxa of Delphinium in Türkiye and Their Taxonomic Importance

Abstract

Pollen micromorphology of 12 taxa belonging to genus Delphinium L. (Ranunculaceae) species was investigated by light microscopy and scanning electron microscopy. Pollen slides were prepared using the Wodehouse technique. Measurements were based on 30 or more pollen grains per specimen. For scanning electron microscopy studies, dried pollen grains were transferred to aluminium stubs and coated with gold for 3 min in a sputter-coater. The pollen grains of Delphinium taxa are radially symmetric and isopolar. The pollen grains of the taxa are prolate–spheroidal, subprolate with the polar axes 24.51–36.24 μm and the equatorial diameter 22.13–30.43 μm. The pollen grains are tricolpate rarely tetracolpate (D. thrikeana). Colpi were usually long and narrow (Clg: 18.46–28.73 μm; Clt: 5.17–9.31 μm) and granulate. Pollen grains show microechinate, microechinate-perforate, microechinate-verrucate. Exine is subtectate and has a thickness of 0.99–1.58 μm. Ectexine is thicker than endexine. Intine has a thickness of 0.39–0.69 μm. As result of this study, some pollen characteristic as pollen shape, aperture type, and ornamentations are shown to vary within the genus. Especially, pollen surface ornamentation has systematic value to distinguish taxa from each other. Cluster and principal component analyses supported the separation of taxa into three types. Type 1 and Type 2 differed mainly in pollen size and aperture dimensions, while D. saccatum (Type 3) was characterized by thicker exine and intine layers. Taxa formerly classified under Consolida clustered together as Type 2, indicating consistent morphological differentiation within the genus Delphinium. These statistical results support the taxonomic value of quantitative pollen traits.

Keywords

Supporting Institution

Science Application and Research Center, University of Yuzuncuyil, Van

Ethical Statement

The authors declare that this study complies with research and publication ethics. This study does not require an ethics committee approval as it does not involve any human or animal subjects, surveys, or clinical data. Furthermore, there is no conflict of interest between the authors.

Thanks

The author thanks Dr. Ihsan Nuri Akkuş (Science Application and Research Center, University of Yuzuncuyil, Van), who is a helper in taking electron photographs of pollen grain surfaces. In addition, this article includes pollen morphology results from PhD thesis of Süleyman AKSU.

References

P. H. Davis, Flora of Turkey and the East Aegean Islands, vol. 1, pp. 108–134. Edinburgh: Edinburgh University Press, 1965.
R. Illarslan, “Türkiye’nin Delphinium L. (Ranunculaceae) cinsinin revizyonu,” Turkish Journal of Botany, vol. 20, pp. 133–159, 1996.
R. Illarslan, “Türkiye'nin Delphinium L. (Ranunculaceae) taksonlarının Türkiye’deki yayılışı,” Turkish Journal of Botany, vol. 14, pp. 190–202, 1990.
G. Erdtman, Pollen Morphology and Plant Taxonomy: Angiosperms. Stockholm: Almqvist and Wiksell, 1952.
G. Erdtman, “Ranunculaceae,” in Handbook of Palynology, p. 110. New York: Hafner Publishing Co, 1969.
G. C. S. Clarke, W. Punt, and P. P. Hoen, “The Northwest European pollen flora, 51 Ranunculaceae,” Rev. Palaeobot. Palynol., vol. 69, pp. 117–271, 1991, doi: 10.1016/0034-6667(91)90071-A.
B. Aytuğ, S. Aykut, N. Merev, and G. Edis, İstanbul Çevresi Bitkilerinin Polen Atlası. İstanbul: İstanbul Üniversitesi Orman Fakültesi Yayınları, Yayın No: 1650, O. F. Yayın No: 174, Kutulmuş Matbaası, 1971.
S. Lee and S. A. Blackmore, “Palynotaxonomic study of the genus Trollius (Ranunculaceae),” Grana, vol. 31, pp. 81–100, 1992, doi: 10.1080/00173139209430728.

M. Kurşat, N. Özdağ, B. Başer, F. Özbey, and İ. Emre, “A palynological investigation of some taxa of the genus Ranunculus L. (Ranunculaceae) in Turkey and its taxonomic value,” Palynology, vol. 46, no. 4, pp. 1–13, 2022, doi: 10.1080/01916122.2022.2095053.
M. J. Noor, M. Ahmad, R. Asghar, A. Kanwal, and S. Pervaiz, “Palynological studies of cultivated plant species at University of Arid Agriculture, Rawalpindi, Pakistan,” Asian J. Plant Sci., vol. 3, pp. 476–479, 2004, doi: 10.3923/ajps.2004.476.479.
W. Oberschneider, “Pollen morphologie der Ranunculanae,” M.S. thesis, Universität Wien, Wien, 1998.
Ş. Doğanay, “Türkiye’nin bazı Consolida (DC.) S.F. Gray (Ranunculaceae) taksonlarının polen morfolojisi,” M.S. thesis, Selçuk Üniversitesi Fen Bilimleri Enstitüsü, Konya, 2005.
B. Bursali and C. Doğan, “Pollen morphology of some Delphinium L. (Ranunculaceae) taxa in Turkey,” Hacettepe J. Biol. Chem., vol. 34, pp. 1–17, 2005.
J. Simon and C. Blanche, “Estudio palinológico del género Consolida (DC.) S. F. Gray (Ranunculaceae) en la Península Ibérica, Actas de Palinología, pp. 156–160, 1986.
P. E. DuPasquier, V. Andro-Durand, L. Batory, W. Wang, F. Jabbour, “ Nomenclatural revision of Delphinium subg. Consolida (DC.) Huth (Ranunculaceae). PhytoKeys, 180:81–110, 2021. doi: 10.3897/phytokeys.180.67126.
R. P. Wodehouse, Pollen Grains. New York: McGraw-Hill, 1935, pp. 106–109.
H. Halbritter, S. Ulrich, F. Grímsson, M. Weber, R. Zetter, M. Hesse, R. Buchner, M. Svojtka, A. Frosch-Radivo, Illustrated Pollen Terminology, 2nd ed. Berlin/Heidelberg: Springer, 2018, p. 483, doi: 10.1007/978-3-319-71365-6.
Mayer, B & K. Biesinger, Modern cloud platforms for collaborative scientific data analysis. Journal of Data Science Tools, 4, 45–62. 2023.
J.C. Gower, A general coefficient of similarity and some of its properties. Biometrics, 27(4), 857–871. 1971. https://doi.org/10.2307/2528823
Sneath, P.H.A & R. R. Sokal, Numerical taxonomy: The principles and practice of numerical classification. San Francisco: W. H. Freeman. 1973.
Everitt, B.S., Landau, S., Leese, M., & D. Stahl, Cluster analysis (5th ed.). Chichester: Wiley. 2011.
Jolliffe, I.T & J. Cadima, Principal component analysis: A review and recent developments. Philosophical Transactions of the Royal Society A, 374(2065), 20150202. 2016. https://doi.org/10.1098/rsta.2015.0202
J. Nowicke and J.A. Skvarla, “Palynological study of the genus Helleborus L. (Ranunculaceae),” Grana, vol. 22, pp. 129–140, 1983.
B. Bursali, “Türkiye’nin bazı Delphinium L. (Ranunculaceae) taksonlarının polen morfolojisi,” M.S. thesis, Hacettepe Üniversitesi Fen Bilimleri Enstitüsü, Ankara, 2001.
A. Perveen and M. Qaiser, “Pollen flora of Pakistan-Ranunculaceae,” Pakistan Journal of Botany, vol. 38, no. 3, pp. 499–509, 2006.
F. Jabbour and S.S. Renner, “A phylogeny of Delphinieae (Ranunculaceae) shows that Aconitum is nested within Delphinium and that Late Miocene transitions to long life cycles in the Himalayas and Southwest China coincide with bursts in diversification,” Mol. Phylogenet. Evol., vol. 62, pp. 928–942, 2012, doi: 10.1016/j.ympev.2011.12.005.
F. Jabbour and S.S. Renner, “Consolida and Aconitella are an annual clade of Delphinium (Ranunculaceae) that diversified in the Mediterranean basin and the Irano-Turanian region,” Taxon 60(4): 1029–1040, 2011. https://doi.org/10.1002/tax.604007
M. Hasanbarani and F. Sharifnia, “Morphological and epidermal surface characters for taxonomic significance of some Delphinium species (Ranunculaceae) in Iran,” presented at The 7th National Congress on Biology and Natural Sciences of Iran, 2020.
K. Faegri and J. Iversen, Textbook of Pollen Analysis, 3rd ed. Copenhagen: Munksgaard, 1975.
P. D. Moore and J.A. Webb, An Illustrated Guide to Pollen Analysis. London: Hodder and Stoughton, 1978.
M. Pakravan, “Palynological study of the genus Consolida (Ranunculaceae) in Iran,” Nova Biologica Reperta, vol. 3, no. 2, pp. 177–183, 2016, doi: 10.21859/acadpub.nbr.3.2.177.
R. Hasani, A. Mazooji, & F. Sharifnia, “Biosystematics study of Consolida persica (Boiss.) Schrod. & Consolida rugulosa (Boiss.) Schrődinger (Ranunculaceae) in Iran”. Australian Journal of Basic and Applied Sciences, 5(6), 1160-1166, 1991.
M. Hasanbarani, F. Sharifnia, & M. Assadi, “Pollen morphology of Delphinium in Iran”. Iranian Journal of Biological Science, 14, 35-53, 2019.
B. Aytug, Polen Morfolojisi ve Türkiye'nin Önemli Gymnospermleri Üzerinde Palinolojik Araştırmalar. İstanbul Üniversitesi Orman Fakültesi Yayınları, İstanbul (İ.Ü Yayın No: 11, O.F. Yayın No: 1261), 1967.

Details

Primary Language

English

Subjects

Botany (Other)

Journal Section

Research Article

Authors

Birol Başer ^*
0000-0002-9305-8759
Türkiye

Süleyman Aksu
0009-0006-3050-1000
Türkiye

Murat Kürşat
0000-0002-0861-4213
Türkiye

Ozan Bekmezci
0000-0001-7972-3911
Türkiye

Publication Date

June 30, 2026

Submission Date

September 23, 2025

Acceptance Date

June 24, 2026

Published in Issue

Year 2026 Volume: 16 Number: 1

DOI

https://doi.org/10.17678/beuscitech.1787364

IZ

https://izlik.org/JA85SG97NX

Cite

RIS / Bibtex

APA

Başer, B., Aksu, S., Kürşat, M., & Bekmezci, O. (2026). Pollen Micromorphology of 12 Taxa of Delphinium in Türkiye and Their Taxonomic Importance. Bitlis Eren University Journal of Science and Technology, 16(1), 1-15. https://doi.org/10.17678/beuscitech.1787364

AMA

1.Başer B, Aksu S, Kürşat M, Bekmezci O. Pollen Micromorphology of 12 Taxa of Delphinium in Türkiye and Their Taxonomic Importance. Bitlis Eren University Journal of Science and Technology. 2026;16(1):1-15. doi:10.17678/beuscitech.1787364

Chicago

Başer, Birol, Süleyman Aksu, Murat Kürşat, and Ozan Bekmezci. 2026. “Pollen Micromorphology of 12 Taxa of Delphinium in Türkiye and Their Taxonomic Importance”. Bitlis Eren University Journal of Science and Technology 16 (1): 1-15. https://doi.org/10.17678/beuscitech.1787364.

EndNote

Başer B, Aksu S, Kürşat M, Bekmezci O (June 1, 2026) Pollen Micromorphology of 12 Taxa of Delphinium in Türkiye and Their Taxonomic Importance. Bitlis Eren University Journal of Science and Technology 16 1 1–15.

IEEE

[1]B. Başer, S. Aksu, M. Kürşat, and O. Bekmezci, “Pollen Micromorphology of 12 Taxa of Delphinium in Türkiye and Their Taxonomic Importance”, Bitlis Eren University Journal of Science and Technology, vol. 16, no. 1, pp. 1–15, June 2026, doi: 10.17678/beuscitech.1787364.

ISNAD

Başer, Birol - Aksu, Süleyman - Kürşat, Murat - Bekmezci, Ozan. “Pollen Micromorphology of 12 Taxa of Delphinium in Türkiye and Their Taxonomic Importance”. Bitlis Eren University Journal of Science and Technology 16/1 (June 1, 2026): 1-15. https://doi.org/10.17678/beuscitech.1787364.

JAMA

1.Başer B, Aksu S, Kürşat M, Bekmezci O. Pollen Micromorphology of 12 Taxa of Delphinium in Türkiye and Their Taxonomic Importance. Bitlis Eren University Journal of Science and Technology. 2026;16:1–15.

MLA

Başer, Birol, et al. “Pollen Micromorphology of 12 Taxa of Delphinium in Türkiye and Their Taxonomic Importance”. Bitlis Eren University Journal of Science and Technology, vol. 16, no. 1, June 2026, pp. 1-15, doi:10.17678/beuscitech.1787364.

Vancouver

1.Birol Başer, Süleyman Aksu, Murat Kürşat, Ozan Bekmezci. Pollen Micromorphology of 12 Taxa of Delphinium in Türkiye and Their Taxonomic Importance. Bitlis Eren University Journal of Science and Technology. 2026 Jun. 1;16(1):1-15. doi:10.17678/beuscitech.1787364