Physiological and biochemical effects of 2.4-D herbicide in wheat (Triticum aestivum L.) varieties

Abstract

In this work were investigated the physiological and biochemical responses depended on toxic effect caused by different concentrations of herbicides called as 2.4-Dichlorophenoxyacetic acid (2.4-D) herbicide in the seedlings that belong to three wheat varieties. The seeds which belong to wheat (Triticum aestivum L. Bayraktar cv., İkizce cv. and Tosunbey cv.) were used as plant material. 15-day old seedlings for each wheat variety were divided into 4 groups consisting of the same number of seedlings and four variety doses of herbicide (0, 100 µM, 300 µM ve 1000 µM) were applied for them. In these applications for seedlings was preferred hydroponic surrounding to root. Although three varieties increased the growth of root and shoot elongation from the growth parameters of the seedlings, it leaded to a decrease in growth parameters in general in this herbicide. Although 2.4-D in the leaves caused an increase in 100 μM dose for only the Bayraktar in amount of chlorophyll a+b. In ones except these, there has always been a decreased. The amount of carotenoid resulted in the reduction of three varieties. Amount of MDA increased in all three varieties. Glutathione (GSH) / oxidized glutathione (GSSG) ratios in leaves increased in three varieties. The superoxide dismutase (SOD) activity in the leaves increased at the Bayraktar and decreased in the İkizce and the Tosunbey. Catalase (CAT) activity showed to a decrease in three varieties. As a result, it was determined that the 2.4-D, which was toxic for wheat plants even in very low concentrations.

Keywords

• Tritucum aestivum L.
• Herbicide
• Oxidative stress
• 2.4-D

References

1. M. Güngör, “Investigating the Importance of Chemical Weeding in Maize Cultivation Areas in the City of Adana and The Emerging Problems”, postgraduate thesis, Çukurova University, Institute of Science, Adana, 2005.
2. [2] L. Mander and H. W. Liu, “Comprehensive Natural Products II Chemistry and Biology”. Elsevier Oxford 3: 205–236, 2010.
3. [3] M. S. Başaran and A. T. Serim, “Degradation of Herbicides by Soil”, Selçuk University. Selçuk Journal of Agriculture and Food Sciences 24 (2): 54-61, 2010.
4. [4] C. M. Santos and M. A. Silva, “Physiological and biochemical responses of sugarcane to oxidative stress induced by water deficit and paraquat”, Acta Physiol. Plant 37: 172, 2015.
5. [5] D. M. Pazmın᷈o, M. Rodriguez-Serrano, M. C. Romero-Puertas, A. Archilla-Ruız, L. A. Del Rio and L. M. Sandalıo, "Differential response of young and adult leaves to herbicide 2,4-dichlorophenoxyacetic acid in pea plants: role of reactive oxygen species" Plant Cell and Environment 34: 1874-1889, 2011.
6. [6] J. D. M. Belgers, R. J. V. Lieverloo, L. J. T. V. Pas and P. J. V. D. Brink, Effects of the herbicide 2,4-D on the growth of nine aquatic macrophytes. Aquatic Botany 86: 260-268, 2007.
7. [7] V. Alexieva, S. Ivanov, I. Sergiev and E. Karanov, “Interaction Between Stresses”. Bulg. J. Plant Physiol. Special Issue 1-7, 2003.
8. [8] Y. Sunohara, S. Shirai, N. Wongkantrakorn and H. Matsumoto, “Sensitivity and physiological responses of Eleusine indica and Digitaria adscendes to herbicide quinclorac and 2,4-D”, Environmental and Experimental Botany 68: 157-164, 2010.

9. [9] Q. Wang, X. Que, R. Zheng, Z. Pang, C. Li and B. Xiao, “Phytotoxicity assessment of atrazine on growth and physiology of three emergent plants”, Environ Sci Pollut Res 22: 9646-9657, 2015.
10. [10] A. Kaya and Z. B. Doğanlar, “Exogenous jasmonic acid induces stress tolerance in tobacco (Nicotiana tabacum) exposed to imazapi”, Ecotoxicology and Enviromental Safety, 124: 470-479, 2016.
11. [11] G. B. Akbulut, E. Yiğit, A. Kayac and A. Aktas, “Effects o f salicylic acid and organic selenium on wheat (Triticum aestivum L.) exposed to fenoxaprop-p-ethyl”, Ecotoxicology and Environmental Safety 148: 901–909, 2018. [12] A. S. Lukatkin, A. N. Gar’kova, A. S. Bochkarjova, O. V. Nushtaeva and J. A. T. Silva, “Treatment with the herbicide TOPIK induces oxidative stress in cereal leaves,” Pesticide Biochemistry and Physiology 105: 44-49, 2013.
12. [13] F. H. Witham, D. F. Blaydes and R. M. Dewlin, “Experiments in Plant Physiology”, New york, Von Nonstrand Reinhold Company 55-56, 1971.
13. [14] O. Yilmaz, S. Keser, M. Tuzcu, M. Guvenc, B. Cetintas, S. Irtegun, H. Tastan and K. Sahin, “A Practical HPLC Method to Measure Reduced (GSH) and Oxidized (GSSG) Glutathione Concentrations in Animal Tissues,” Journal of Animal and Veterinary Advances 8(2) 343-347, 2009.
14. [15] F. Karataş, M. Karatepe and A. Baysar, “Determination of free malondialdehyde in human serum by high performence liquid chromatography”, Anal Biochemistry 311: 76-79, 2002.
15. [16] G. Mourente, D. R. Tocher, E. Diaz, A. Grau and E. Pastor, “Relationships between antioxidants, antioxidant enzyme activities and lipid peroxidation products during early development in Dentex dentex eggs and larvae,” Aquaculture 179: 309–324, 1999. [17] H. Aebi, “Catalase in Vitro”, Method Enzym. 105: 121-126, 1984.
16. [18] G. B. Akbulut and E. Yiğit, “The changes in some biochemical parameters in Zea mays cv. “Martha F1” treated with atrazine”, Ecotoxicology and Environmental Safety 73: 1429-1432, 2010.
17. [19] M. C. Romero-Puertas, I. Mccarthy, M. Gomez, L. M. Sandalıo, F. J. Corpas, L. A. Del Rio and J. M. Palma, “Reactive oxygen species-mediated enzymatic systems involved in the oxidative action of 2,4-dichlorophenoxyacetic acid”, Plant,Cell and Environment 27: 1135-1148, 2004.