The relationship between nitric oxide and cadmium toxicity in wheat (triticum aestivum L.) seedlings

Year 2020, Volume: 10 Issue: 2, 62 - 66, 28.12.2020

Songül Çanakcı Gülengül Ayşe Dilek Özşahin Kireçci Tuba Okutan

https://doi.org/10.17678/beuscitech.763348

Abstract

In this study, biochemical responds against different cadmium concentrations (25 µM, 50 µM and 75 µM) in seedlings belonging to three wheat (Triticum aestivum L.) varieties applied to different SNP (25 µM and 50 µM) concentrations. As the material of the study, fifteen days old seedlings of wheat (Triticum aestivum L.) were used. In all applications carried out to the seedlings, hydroponic method was preferred. The seedlings were divided into three groups in which pretreatment of SNP for 48 hours were done. After that, different concentrations of cadmium were applied to these three groups to except controls (pure water and SNPs). In addition, reduced glutathione (GSH) / oxidized glutathione (GSSG) ratio, catalase (CAT) with superokside glutathione (SOD) activities were detected in the leaves.
According to the obtained results, (GSH) / (GSSG) ratio reduced in all three varieties; CAT activity was reduced in Bayraktar and Ikizce, but it was increased in Tosunbey. SOD activity was increased all three varieties. The most prominent responses of SOD enzyme activity in the leaves of wheat seedlings were determined in Tosunbey wheats. When the results are evaluated, generally, 50 µM of SNP pre-application was found as more successful than 25 µM of SNP application in terms of attenuating Cd toxicity. SNP was found to have a mitigating effect against Cd depending on the dose.

Keywords

Cadmium toxicity, Nitric oxide, Oxidative damage, SNP, Wheat (Triticum aestivum L.)

References

• Aebi, H., 1984. Catalase in Vitro, Method Enzym, 105,121-126.
• Amooaghaie, R., Faezeh, Z.M., and Shekoofeh, E., 2017. Role of two-sided crosstalk between NO and H2S on improvement of mineral homeostasis and antioxidative defense in Sesamum indicum under lead stress. Ecotoxicology and Environmental Safety. 139. 210-218. 10.1016/j.ecoenv.2017.01.037.
• Beligni, M.V., and Lamattina, L., 1999a. Nitric oxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissues, Planta, 208, 337-344.
• Beligni, M.V., and Lamattina, L., 1999b. Is nitric oxide toxic or protective, Trends Plant Sci., 4, 299-300.
• Benavides, M.P., Gallego, S.M., and Tomaro, M.L., 2005. Cadmium toxicity in plants, Braz. J. Plant Physiol., 17 (1), 21-34.
• Chaudhary, S., and Sharma Y.K., 2009. Interactive studies of potassium and copper with cadmium on seed germination and early seedling growth in maize (Zea mays L.), Journal of Environmental Biology, 30, 427-432.
• Gill, S.S., Hasanuzzaman, M., Nahar, K., Macovei, A., and Tuteja, N., 2013. Importance of nitric oxide in cadmium stress tolerance in crop plants, Plant Physiology and Biochemistry, 63,254-261.
• Hassan, M.J., Shao, G., and Zhang, G., 2005. Influence of Cadmium Toxicity on Growth and Antioxidant Enzyme Activity in Rice Cultivars with Different Grain Cadmium Accumulation, Journal of Plant Nutrition, 28 (7),1259-1270.
• Hegedus, A., Erdei, S., and Horváth, G., 2001. Comparative Studies of H2O2 Detoxifying Enzymes in Green and Greening Barley Seedings under Cadmium Stress, Plant Science, 160, 1085 -1093.
• Hufton, C.A., Besford, R.T., and Wellburn, A.R., 1996. Effects of NO (+NO2) pollution on growth, nitrat reductase activities and associated protein contents in glasshouse lettuce grown hydroponically in winter CO2 enrichment, New Phytologist, 133, 495-501.
• Jouili, H., and El Ferjani, E., 2004. Effects of copper excess on superoxide dismutase, catalase and peroxidase activities in sunflower seedlings (Helianthus anuus L.), Acta Physiologiae Plantarum, 26, 29-35.
• Kaya, A., and Z.B. Doganlar. 2016. Exogenous jasmonic acid induces stres tolerance in tobacco (Nicotiana tabacum) exposed to imazapic. Ecotoxicol. & Environ. Safety, 124, 470-479.
• Khairy, A.I., Oh, M.J., Lee, S.M., Kim, D.S., and Roh, K.S., 2016. Nitric oxide overcomes Cd and Cu toxicity in in vitro-grown tobacco plants through increasing contents and acitvities of rubisco and rubisco activase, Biochimie Open, 2,41-51.
• Kotapati, K.V., Palaka, B.K., and Ampasala, D.R., 2017. Alleviation of nickel toxicity in finger millet (Eleusine coracana L.) germinating seedlings by exogenous application of salicylic acid and nitric oxide Crop J, 5: 240-250.
• Laspina, N.V., Groppa, M.D., Tomaro, M.L., and Benavides, M.P., 2005. Nitric oxide protects sunflower leaves against Cd- induced oxidative stress, Plant Science, 168 (7), 252-260.
• Leshem, Y.Y., and Haramaty, E., 1996. The Characterisation and Contrasting Effects of the Nitric Oxide Free Radical in Vegetative Stress and Senescence of Pisum sativum L. Foliage, Journal of Plant Physiology, 148, 258-263.
• Liang, Y., Zheng, P., Li, S., Li, K., and Xu, H., 2018. Nitrate reductase dependent NO production is involved in H2S – induced nitrase stress tolerance in tomato via activation of antioxidant enzymes, Scientia Horticulturae, 229, 207- 214.
• Mourente, G., Tocher, D., R., Diaz, E., Grau, A., and Pastor, E., 1999. Relationships between antioxidants, antioxidant enzyme activities and lipid peroxidation products during early development in Dentex eggs and larvae, Aquaculture, 179,309-324.
• Nahar, K., Hasanuzzaman, M., Alam, M., Rahman, A., Suzuki, T., and Fujita, M., 2016. Polyamine and nitric oxide crosstalk: Antagonistic effests on cadmium toxicity in mung bean plants through upregulating the metal detoxification, antioxidant defense and methyglyoxal detoxification systems, Ecotoxicology and Environmental Safety, 126, 245-255.
• Neill, S.J., Desikan, R., and Hancock, J.T., 2003. Nitric Oxide Signalling in Plants, New Phytologist, 159, 1469-1481.
• Okcu, M., Tozlu, E., Kumlay, A.M., and Pehluvan, M., 2009. Alınteri Dergisi, 17 (B), 14-26, ISSN, 1307-3311.
• Okturen Asri, F., Sonmez, S. ve Citak, S., 2007. Kadmiyumun çevre ve insan sağlığı üzerine etkileri, Batı Akdeniz Tarımsal Araştırma Enstitüsü/ANTALYA, Akdeniz Üniversitesi Ziraat Fakültesi Toprak Bölümü/ ANTALYA. http://batem.gov.tr/yayinlar/derim/2007/32-39.pdf.
• Romero-Puertas, M.C., Corpas, F.J., Rodríguez-Serrano, Gόmez, M., del Rio, L.A., and Sandalio, L.M., 2007. Differential expression and regulation of antioxidative enzymes by cadmium in pea plants, Journal of Plant Physiology, 164, 1346-1357.
• Santos, C.M., and Silva, M.A., 2015. Physiological and biochemical responses of sugarcane to oxidative stress induced by water deficit and paraquat, Acta Physiol. Plant, 37, 172.
• Verma S., and Dubey R.S., 2003. Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants, Plant Sci., 164, 645-655.
• Wang, Q., Que, X., Zheng, R., Pang, Z., Li, C. and Xiao, B., 2015. Phytotoxicity assessment of atrazine on growth and physiology of three emergent plants, Environ Sci Pollut Res, 22, 9646-9657.
• Yilmaz, O., Keser, S., Tuzcu, M., Guvenc, M., Cetintaş, B., İrtegun, S., Tastan, H. ve Şahin, K., 2009. A Practical HPLC Method to Measure Reduced (GSH) and Oxidized (GSSG) Glutathione Concentrations in Animal Tissues, Journal of Animal and Veterinary Advances, 8 (2), 343-347.
• Zacchini, M., Rea, E., Tullio, M. ve Agazio, M., 2003. Increased antioxidative capacity in maize calli during and after oxidative stress induced by a long lead treatment, Plant Physiol. Bioch., 41, 49-54.