Hydrodistillation of Nigella Sativa Seed and Analysis of Thymoquinone with HPLC and GC-MS

Abstract

N. sativa seeds, commonly known as black seeds, are for headaches in traditional medicine by many Asian, Middle Eastern and Far Eastern countries. It is used to treat cough, abdominal pain, diarrhea, asthma, rheumatism and other diseases. The seeds contain both fixed and essential oils, proteins, alkaloids and saponin. Much of the biological activity of the seeds has been shown to be due to thymoquinone, the major component of the essential oil, but which is also present in the fixed oil. The essential oil of black cumin seeds, Nigella sativa L., was tested for a possible antioxidant activity. In this study, we measured the amount of thymoquinone compound in distilled water taken from aromatic water. The proportion of thymoquinone passing into the aromatic water show that it is necessary to consume the correct amount of water and essential oil, which is significantly measurable by GC MS and HPLC. Thymoquinone was dedected 790 ppm in aromatic water and 45.78% in essential oil. Sufficient analytical work was performed with this method and the results were shown.

Keywords

• Nigella Sativa,GC-MS, Hydrodistillation, Thymoquinone, HPLC,Essential oil

References

1. Al-Haj, N.A., Shamsudin, M.N., Alipiah, N.M., Zamri, H.F., Ahmad, B., Siddig, I., Rasedee, A. (2010). Characterization of Nigella sativa L. Essential oil-loaded solid lipid nanoparticles. American Journal of Pharmacology and Toxicology. 5(1): 52-57.
2. Aljabre, S.H., Alakloby, O.M., Randhawa, M.A. (2015). Dermatological effects of Nigella sativa. Journal of Dermatology and Dermatologic Surgery. 19(2): 92-98. Bourgou, S., Pichette, A., Marzouk, B., Legault, J. (2010). Bioactivities of black cumin essential oil and its main terpenes from Tunisia. South African Journal of Botany. 76(2) : 210-216.
3. Burits, M., Bucar ,F. (2000). Antioxidant activity of Nigella sativa essential oil. Phytotherapy research. 14(5): 323-328.
4. El‐Mahdy, M.A., Zhu, Q., Wang, Q.E., Wani, G., Wani, A.A. (2005). Thymoquinone induce sapoptosis through activation of caspase‐8 and mitochondrialevents in p53‐null myeloblastic leukemia HL‐60 cells. International Journal of Cancer. 117(3):409-417.
5. El-Tahir, KEDH, Bakeet, D.M. (2006). The black seed Nigella sativa Linnaeus A mine formulticures a pleafor urgent clinical evaluation of its volatile oil. Journal of Taibah University Medical Sciences. 1(1): 1-19.
6. Forouzanfar, F., Bazzaz, B.S.F., Hosseinzadeh, H. (2014). Black cumin (Nigella sativa) and its constituent (thymoquinone) a review on antimicrobial effects. Iranian Journal of Basic Medical Sciences. 17(12): 929.
7. GaliMuhtasib, H., Diab Assaf, M., Boltze, C., Al-Hmaira, J., Hartig, R., Roessner, A., Schneider Stock, R. (2004). Thymoquinone extracted from blackseed triggers apoptotic cell death in human colorectal cancer cells via a p53-dependent mechanism. International Journal of Oncology. 25(4):857-866.
8. Goyal, S.N., Prajapati, C.P., Gore, P.R., Patil, C.R., Mahajan, U.B., Sharma, C., Ojha, S.K. (2017). Therapeutic potential and pharmaceutical development of thymoquinone: a multitargeted molecule of natural origin. Frontiers in Pharmacology. 8: 656.

9. Güzelsoy, P., Aydın, S., Başaran, N. (2018). Çörek Otunun (NigellaSativa L.) Aktif Bileşeni Timokinonun İnsan Sağlığı Üzerine Olası Etkileri. Journal of Literature Pharmacy Sciences. 7(2): 118-135.
10. Harzallah, H.J., Kouidhi, B., Flamini, G., Bakhrouf, A., Mahjoub, T. (2011). Chemical composition, antimicrobial potential against cariogenic bacteria and cytotoxic activity of Tunisian Nigella sativa essential oil and thymoquinone. Food Chemistry. 129(4) : 1469-1474.
11. Houghton, P.J., Zarka, R., de las Heras, B., Hoult, JRS. (1995). Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Plantamedica. 61(01): 33-36.
12. Kaseb, A.O., Chinnakannu, K., Chen, D., Sivanandam, A., Tejwani, S., Menon, M., Reddy, G. P V. (2007). Androgen receptor and E2F-1 targeted thymoquinone therapy for hormone refractory prostate cancer. Cancer Research. 67(16): 7782-7788.
13. Kiralan, M. (2014). Changes in volatile compounds of blackcumin (Nigellasativa L.) seed oil during thermal oxidation. International journal of Food Properties. 17(7): 1482-1489.
14. Nickavar, B., Mojab, F., Javidnia, K., Amoli, M.A.R. (2003). Chemical composition of the fixed and volatile oils of Nigella sativa L. from Iran. Zeitschriftfür Naturforschung C. 58(9-10): 629-631.
15. Odeh, F., Ismail, S.I., Abu Dahab, R., Mahmoud, I.S., Al Bawab, A. (2012). Thymoquinone in liposomes a study of loading efficiency and biological activity towards breast cancer. Drug delivery. 19(8): 371-377.
16. Salem, M.L. (2005). Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. International Immunopharmacology. 5 (13-14): 1749-1770.
17. Selin, I., Kartal, M., Erdem, S.A. (2017). Quantitative analysis of thymoquinone in Nigella Sativa L.(Black Cumin) seeds and commercial seed oils and seed oil capsules fromTurkey. Ankara Üniversitesi Eczacılık Fakültesi Dergisi. 41(1).
18. Shoieb, A.M., Elgayyar, M., Dudrick, P.S., Bell, J.L., Tithof, P.K. (2003). In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone. International Journal of Oncology. 22(1): 107-113.
19. Sultan, M.T., Butt, M.S., Anjum, F.M., Jamil, A., Akhtar, S., Nasir, M. (2009). Nutritional profile of indigenous cultivar of black cumin seeds and antioxidant potential of its fixed and essential oil. Pakistan Journal Botany. 41(3): 1321-1330.
20. Worthen, D.R., Ghosheh, O.A., Crooks, P.A. (1998). The in vitro anti-tumoractivity of some crude and purified components of blackseed, Nigella sativa L. Anticancer Research. 18(3A): 1527-1532.