Kimyasal Olarak Modifiye Edilmiş Kitosanın Kuvars Kristal Mikroterazi Kullanarak Antibiyofilm Aktivitesinin Değerlendirilmesi

Abstract

Biyomalzemeler üzerinde büyüme ve hücresel bağlanma ile biyofilm oluşumunu engellemek için, yeni biyomalzemeler geliştirilebilir. Bu şekilde biyomalzemeler yeni özellikler kazanabilir. Bu çalışmada, plazma modifiye kitosan (PCh), 5-etoksi-2-metil-benzofuran-3-karboksilik asit (E1) ile kimyasal olarak modifiye edildi. Kimyasal olarak modifiye edilmiş PCh ve PCh-E1 filmlerinin yapıları, X-ışını fotoelektron spektroskopisi (XPS), Fotolüminesans spektroskopisi (PL) ve Fourier dönüşümü kızılötesi spektroskopisi (FTIR) ile incelendi. PCh ve PCh-E1'in elektrospun nanolifleri, destek polimer polivinil alkol (PVA) varlığında yerinde elektroeğirme ve kuvars kristal mikroterazi (QCM) kullanılarak, QCM elektrot yüzeyinde mikrogram düzeyinde üretildi. Elektrospun nanoliflerin morfolojileri ve çapları Taramalı elektron mikroskobu (SEM) ile incelendi. PVA, PVA/PCh ve PVA/PCh-E1'in ortalama lif çapları ve standart sapmaları sırasıyla 280.0 ± 58.9, 104.5 ± 35.9 ve 99.4 ± 21.9 nm olarak belirlendi. PVA/PCh nanoliflerinden daha ince çapa sahip PVA/PCh-E1 nanolifler elde edildi. Nanolifler ile kaplanmış QCM elektrot yüzeylerinin Pseudomonas aeruginosa (P. aeruginosa) karşı antibiyofilm aktiviteleri, QCM ile bağlantılı bir akış hücresi kullanılarak değerlendirildi. PVA/PCh-E1 nanolifleri ile kaplanmış QCM elektrodunda (ΔF: -13709.5 Hz, Δm: 530.3 μg cm−2), PVA/PCh nanolifler ile kaplanmış QCM elektroduna (ΔF: -14552.7 Hz, Δm: 563.5 μg cm−2) göre daha az negatif frekans kayması ve kütle artışı belirlendi. QCM sonuçları, PVA/PCh-E1 nanoliflerinin, E1 bileşiğinin olası bir katkısı nedeniyle biyofilm oluşumunu önemli ölçüde azalttığını gösterdi.

Keywords

• biyofilm
• kitosan
• elektroeğirme
• lif
• kuvars kristal mikroterazi

The Assessment of Antibiofilm Activity of Chemically Modified Chitosan Using Quartz Crystal Microbalance

Abstract

New biomaterials can be developed to prevent the formation of biofilms on biomaterials through growth and cellular attachment. In this way, biomaterials can gain new properties. In this study, the plasma modified-chitosan (PCh) was chemically modified with 5-ethoxy-2-methyl-benzofuran-3-carboxylic acid (E1). The structures of chemically modified PCh-E1 and PCh films were studied by X-ray photoelectron spectroscopy (XPS), Photoluminescence spectroscopy (PL), and Fourier transform infrared spectroscopy (FTIR). Electrospun nanofibers of PCh and PCh-E1 were produced at the microgram level onto the QCM electrode surface using in situ electrospinning and quartz crystal microbalance (QCM) in the presence of a support polymer polyvinyl alcohol (PVA). The morphologies and diameters of electrospun nanofibers were investigated by the Scanning electron microscopy (SEM). The average fiber diameters and standard deviations of PVA, PVA/PCh, and PVA/PCh-E1 were determined as 280.0 ± 58.9, 104.5 ± 35.9, and 99.4 ± 21.9 nm, respectively. Finer diameter PVA/PCh-E1 nanofibers were obtained from the PVA/PCh nanofibers. Antibiofilm activities against Pseudomonas aeruginosa (P. aeruginosa) of a QCM electrode surfaces coated with nanofibers were evaluated using the flow cell associated with the QCM. When QCM electrode coated with the PVA/PCh-E1 nanofibers was used, less negative frequency shift and the mass increase in (ΔF: -13709.5 Hz, Δm: 530.3 μg cm−2) was detected compared to the QCM electrode coated with PVA/PCh nanofibers (ΔF: -14552.7 Hz, Δm: 563.5 μg cm−2). QCM results showed that PVA/PCh-E1 nanofibers significantly decrease biofilm formation, due to a possible contribution of E1 compound.

Keywords

• biofilm
• chitosan
• electrospinning
• fiber
• quartz crystal microbalance

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