Phalaenopsis orkidelerinin in vitro üretimi

Abstract

Phalaenopsis, ev dekorasyonu için iç mekanlarda yetiştirilen en popüler orkidelerdir. Bu nedenle, Phalaenopsis'in çoğaltılması ve yetiştirilmesi ticari yetiştiriciler için büyük önem taşımaktadır. Phalaenopsis'in mikro çoğaltılmasındaki iyileştirmeler, kayıpların ve israfın azaltılmasını sağlayarak ekonomik faydalar sunabilir. Bu çalışmanın amacı, bir grup ön test-son test modeli kullanarak Phalaenopsis tohumlarının in vitro çimlenmesi üzerinde çeşitli besin ortamlarının ve bazı bitki büyüme düzenleyicilerinin (PGR'ler) etkilerini incelemektir. Araştırmada kullanılan besin ortamları arasında Knudson C (KCM), Lin-demann (LM), Orchimax (-OM), Orchimax + aktif kömür (+OM) ve Murashige & Skoog (MS) yer alırken, PGR'ler olarak 6-Benzilaminopurin (6BA), 6-Furfurylaminopurin (KIN), Adenin hemisülfat (AHS), Thidiazuron (TDZ), 2-İsopentenil adenin (2iP) ve Gibberellik asit (GA3) incelenmiştir. Çimlenen fidelerden elde edilen eksplantlar, doğrudan organogenez işlemine tabi tutularak optimal PGR ve doku fragmanları belirlenmiştir. Sonuçlar, +OM ortamının en kısa çimlenme süresini (gün cinsinden) sağladığını göstermiştir. TDZ konsantrasyonu ile çimlenme yüzdesi arasında ters bir ilişki gözlenmiş, diğer PGR'ler ise konsantrasyon ile pozitif bir korelasyon göstermiştir. Ancak, kontrol ile karşılaştırıldığında çimlenme açısından önemli bir fark bulunmamıştır. Doğrudan organogenez araştırmalarının bulguları, en yüksek üretkenliğe sahip olan ortamın 5.0 ppm 6BA ile zenginleştirilmiş olduğunu ortaya koymuştur. TDZ içeren ortamlar düşük düzeyde bir verimlilik sergilemiş, özellikle 1.0 ppm TDZ grubu, kontrol grubuna kıyasla azalmış etkinlik göstermiştir. Kök uzatma aşamasındaki tüm sitokinin konsantrasyonları, kontrole kıyasla olumlu bir etki göstermiş, ancak bu PGR'ler arasındaki fark istatistiksel olarak anlamlı bulunmamıştır.

Keywords

• besin ortamı
• Bitki büyüme düzenleyicileri (PGR
• doğrudan organogenez
• gibberellik asit (GA3)
• sitokinin

In vitro production of Phalaenopsis orchids

Abstract

Phalaenopsis – known as moth orchids – are the most popular orchids cultivated indoors as decorative house plants. This makes propagation and cultivation of Phalaenopsis important for commercial growers. Enhancements to the micropropagation of Phalaenopsis would have pronounced economic benefits through reduced losses and wastage. We examined the effects of several nutrient media and specific plant growth regulators (PGRs) belonging to the gibberellic acid and cytokinin groups on the in vitro germination of Phalaenopsis seeds, utilizing a single group pretest-posttest model. The effects of several nutrient media such as: Knudson C (KCM), Lindemann (LM), Orchimax (-OM), Orchimax + activated charcoal (+OM), Murashige & Skoog (MS), as well as various PGRs such as 6-Benzylaminopurine (6BA), 6-Furfurylaminopurine (KIN), Adenin hemisulfate (AHS), Thidiazuron (TDZ), 2-Isopentenyl adenine (2iP), and Gibberellic acid (GA3), on the process of germination were also investigated. The explants obtained from the germinating seedlings were subjected to direct organogenesis, and the optimal PGR and tissue fragments were determined. The +OM medium facilitated the shortest germination period (in days). An inverse relationship between the concentration of TDZ and the percentage of germination in the context of the employed PGRs was observed. Apart from TDZ, the remaining PGRs exhibited a positive correlation with concentration. However, no significant difference in germination was observed in comparison to the control. The findings of direct organogenesis investigations revealed that the medium that exhibited the highest productivity was enriched with 5.0 ppm of 6BA. The media containing TDZ exhibited a reduced level of efficiency. Particularly, the group treated with 1.0 ppm of TDZ exhibited reduced efficacy compared to the control group. All concentrations of cytokinin in root elongation stage exhibited a favorable impact in comparison to the control. The variance between these PGRs was not statistically significant.

Keywords

• Cytokinin
• direct organogenesis
• gibberellic acid (GA3)
• nutrient media
• Plant growth regulators (PGRs)

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