Endophytic bacterial diversity of Origanum rotundifolium: isolation and molecular identification of strains

Abstract

Endophytic bacteria in medicinal and aromatic plants provide valuable insights into the microbial diversity of these plants and form the basis for research on their ecological roles and biotechnological potential. In this study, the endophytic bacterial diversity of Origanum rotundifolium Boiss. was determined, and a total of eleven endophytic bacteria were isolated from the root, stem, and leaf tissues of O. rotundifolium. The isolated bacteria were characterized based on certain morphological and biochemical properties. Molecular identification was performed using 16S rRNA gene sequencing. The identified isolates were as follows: Kocuria rosea (OR1), Schumannella sp. (OR2), Micrococcus luteus (OR3, OR7), Bacillus proteolyticus (OR4), Curtobacterium flaccumfaciens (OR5), Staphylococcus sp. (OR6, OR10), Microbacterium proteolyticum (OR8), Microbacterium sp. (OR9), and Actinomyces sp. (OR11). This study reports, for the first time, the diversity of endophytic bacteria isolated from Origanum rotundifolium.

Keywords

• Origanum rotundifolium
• Endophytic bacteria
• 16S rRNA gene sequencing
• Molecular identification
• Medicinal and aromatic plants

Origanum rotundifolium’un endofitik bakteri çeşitliliği: izolasyon ve moleküler tanımlama

Abstract

Tıbbi ve aromatik bitkilerdeki endofitik bakteriler, bu bitkilerin mikrobiyal çeşitliliği hakkında değerli bilgiler sunmakta ve ekolojik rollerinin yanı sıra biyoteknolojik potansiyellerine dair araştırmalar için temel oluşturmaktadır. Bu çalışmada, Origanum rotundifolium Boiss.’un endofitik bakteri çeşitliliği belirlenmiş ve toplamda on bir endofitik bakteri, O. rotundifolium’un kök, gövde ve yaprak dokularından izole edilmiştir. İzole edilen bakteriler, bazı morfolojik ve biyokimyasal özelliklerine göre karakterize edilmiştir. Moleküler tanımlama, 16S rRNA gen dizileme yöntemiyle yapılmıştır. Tanımlanan izolatlar sırasıyla: Kocuria rosea (OR1), Schumannella sp. (OR2), Micrococcus luteus (OR3, OR7), Bacillus proteolyticus (OR4), Curtobacterium flaccumfaciens (OR5), Staphylococcus sp. (OR6, OR10), Microbacterium proteolyticum (OR8), Microbacterium sp. (OR9) ve Actinomyces sp. (OR11) olarak belirlenmiştir. Bu çalışma, Origanum rotundifolium'dan izole edilen endofitik bakteri çeşitliliğini ilk kez rapor etmektedir.

Keywords

• Origanum rotundifolium
• Endofitik bakteri
• 16S rRNA
• Moleküler tanımlama
• Tıbbi aromatik bitki

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