Galanthus woronowii (Amaryllidaceae)'nin antioksidan aktivitesi ve toplam fenolik içeriği.

Abstract

Bitkiler eski çağlardan beri tıbbi amaçlarla kullanılmaktadır. İçerdikleri biyoaktif sekonder metabolitlerden dolayı, bitkiler ilaç keşfi ve gelişimi için oldukça fazla ilgi görmektedir. Bu çalışmada, Galanthus woronowii türüne ait bitki materyalleri sırasıyla hekzan, diklorometan ve etil asetat ile ekstrakte edilmiştir. Çözücü dönerli buharlaştırıcı ile uzaklaştırıldıktan sonra ham ekstraktlar elde edilmiştir. Ekstraktların DPPH serbest radikal giderme, ABTS radikal katyon giderme ve indirgeme gücü aktiviteleri değerlendirilmiştir. Ayrıca toplan fenolik içerik belirlenmiştir. Etil asetat ekstraktının en çok fenolik içerdiği ve en yüksek antioksidan aktivite gösterdiği belirlenmiştir. Bu nedenlerle, bu bitki ümit verici antioksidan ajan olarak kabul edilebilir.

Keywords

• Galanthus woronowii,doğal ürün,antioksidan aktivite,Fenolik içerik

Antioxidant activity and total phenolic contents of Galanthus woronowii (Amaryllidaceae).

Abstract

Plants have been used for medicinal purpose since ancient times. Due to the including bioactive secondary metabolites, plants have gained the great interest for drug discovery and development process. In this work, Galanthus woronowii was extracted with hexane, dichloromethane and ethyl acetate sequentially. After removing of the solvent by rotary evaporator, crude extracts were yielded. Antioxidant activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and reducing power assays were executed on corresponding extracts. In addition, total phenolic content was presented. Ethyl acetate extract included the most phenolic compounds and also it revealed the most antioxidant activity. Hence, this plant could be considered as a promising antioxidant agent.

Keywords

• Galanthus woronowii,antioxidant activity,phenolic contents,natural product

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