Investigation of antiproliferative and apoptotic effects of Rosmarinus officinalis essential oil obtained by hydrodistillation on neuroblastoma cells

Abstract

This study was designed to determine the potential antiproliferative and apoptosis inducing activities of Rosmarinus officinalis essential oil (RE) on neuroblastoma cancer cells. For this purpose, different concentrations of RE were applied to SH-SY5Y cells for 24 hours and cell viability was determined by MTT. In addition, the percentage of early, late and non-apoptotic cells was determined by AnnexinV/propodium iodide staining to determine the induction of apoptosis. In addition, the composition of RE was determined by GC-MS. In MTT assay, it was determined that the viability of SH-SY5Y cells decreased dose-dependently as a result of the application of different concentrations of RE. Moreover, 200 mg/ml RE treatment increased the percentage of cells in the late apoptotic phase. The main compounds of RE were determined as (1R)-2,6,6-Trimethylbicyclo[3.1.1]hept-2-ene, Bicyclo[3.1.1]hept-3-en-2-one, 4,6,6-trimethyl, 1,8-Cineole, Camphor by GC-MS. In conclusion, RE is thought to be an important source of anti-proliferative and apoptosis inducing activity on neuroblastoma cells.

Keywords

• Neuroblastoma
• Rosmarinus officinalis
• Apoptosis

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