The effects of thiamine on in vitro organ development and anthocyanin accumulation in Raphanus sativus

Rumeysa Özbey; Arda Acemi

doi:10.51539/biotech.1912385

The effects of thiamine on in vitro organ development and anthocyanin accumulation in Raphanus sativus

Abstract

Thiamine (Vitamin B1) has an important role as an enzymatic cofactor in metabolic processes such as central carbon pathways, which are necessary for biosynthesis and energy production in plants. The current study investigated concentration-dependent effects of in vitro thiamine treatments on seed germination, organ development, and anthocyanin accumulation in hypocotyls of Raphanus sativus (radish) 'Cherry Belle'. Radish seeds (30 per treatment across 6 replicates) were cultured for 15 days on Murashige and Skoog medium supplemented with six additional thiamine HCl concentrations: 0.4, 1.6, 6.4, 12.8, 25.6, and 51.2 mg L-1. Thiamine supplementation did not result in statistically significant changes in seed germination, whereas concentrations above 6.4 mg L-1 reduced leaf production (to 2.40 leaves per seedling at 12.8 mg L-1). All thiamine treatments enhanced hypocotyl elongation, while treatments above 6.4 mg L-1 induced root elongation, reaching a maximum length of 8.64 cm at 25.6 mg L-1. The treatment at 25.6 mg L-1 gave the maximum seedling vigor index (3737.23 ± 510.44). The treatment at 12.8 mg L-1 induced the highest anthocyanin accumulation in the hypocotyls (3136.05 ± 684.52 µg CGE g-1 DW). The findings suggested that thiamine treatment at specific concentrations may be useful for optimizing early in vitro seedling development and anthocyanin accumulation in radish. Also, the need for further studies to explain the underlying physiological mechanisms responsible for these concentration-dependent effects of thiamine in radish plants was suggested.

Keywords

References

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Details

Primary Language

English

Subjects

Plant Biotechnology, Plant Physiology

Journal Section

Research Article

Authors

Rumeysa Özbey
0009-0006-7996-2545
Türkiye

Arda Acemi ^*
0000-0003-0270-8507
Türkiye

Publication Date

June 29, 2026

Submission Date

March 18, 2026

Acceptance Date

June 20, 2026

Published in Issue

Year 2026 Volume: 7 Number: 1

DOI

https://doi.org/10.51539/biotech.1912385

IZ

https://izlik.org/JA87FX92FC

Cite

RIS / Bibtex

APA

Özbey, R., & Acemi, A. (2026). The effects of thiamine on in vitro organ development and anthocyanin accumulation in Raphanus sativus. Bulletin of Biotechnology, 7(1), 67-73. https://doi.org/10.51539/biotech.1912385

AMA

1.Özbey R, Acemi A. The effects of thiamine on in vitro organ development and anthocyanin accumulation in Raphanus sativus. Bull. Biotechnol. 2026;7(1):67-73. doi:10.51539/biotech.1912385

Chicago

Özbey, Rumeysa, and Arda Acemi. 2026. “The Effects of Thiamine on in Vitro Organ Development and Anthocyanin Accumulation in Raphanus Sativus”. Bulletin of Biotechnology 7 (1): 67-73. https://doi.org/10.51539/biotech.1912385.

EndNote

Özbey R, Acemi A (June 1, 2026) The effects of thiamine on in vitro organ development and anthocyanin accumulation in Raphanus sativus. Bulletin of Biotechnology 7 1 67–73.

IEEE

[1]R. Özbey and A. Acemi, “The effects of thiamine on in vitro organ development and anthocyanin accumulation in Raphanus sativus”, Bull. Biotechnol., vol. 7, no. 1, pp. 67–73, June 2026, doi: 10.51539/biotech.1912385.

ISNAD

Özbey, Rumeysa - Acemi, Arda. “The Effects of Thiamine on in Vitro Organ Development and Anthocyanin Accumulation in Raphanus Sativus”. Bulletin of Biotechnology 7/1 (June 1, 2026): 67-73. https://doi.org/10.51539/biotech.1912385.

JAMA

1.Özbey R, Acemi A. The effects of thiamine on in vitro organ development and anthocyanin accumulation in Raphanus sativus. Bull. Biotechnol. 2026;7:67–73.

MLA

Özbey, Rumeysa, and Arda Acemi. “The Effects of Thiamine on in Vitro Organ Development and Anthocyanin Accumulation in Raphanus Sativus”. Bulletin of Biotechnology, vol. 7, no. 1, June 2026, pp. 67-73, doi:10.51539/biotech.1912385.

Vancouver

1.Rumeysa Özbey, Arda Acemi. The effects of thiamine on in vitro organ development and anthocyanin accumulation in Raphanus sativus. Bull. Biotechnol. 2026 Jun. 1;7(1):67-73. doi:10.51539/biotech.1912385