Investigation of B. subtilis viability at different pH ranges for use in microbial cleaner formulation

Abstract

Until today, several disinfecting methods have been proposed and studied for cleaning and disinfection, containing heavy metals and chlorine, to reduce the biological load on environmental surfaces. Most of the proposed techniques are based on the use of chemical compounds. These methods have proven to be effective in reducing the majority of pathogens, but have been ineffective in preventing the persistence of pollutant microorganisms. Nowadays, increasing demand for natural and green products has led to recognition of cleaning products containing microbial based cultures. Cleaning products containing live microorganisms as active substances are becoming increasingly common in homes and industrial cleaning applications. These products are called "probiotic or microbial" cleaners. Microbial based cleaning products are environmentally cleaning products that contain bacteria or spores that are suitable as active ingredients. The purpose of using these cleaning systems is to prevent the development of pathogens by tolerating the presence of harmless probiotic microorganisms on surfaces. The aim of this study is to investigate the viability of B. subtilis species at different pH values for use in detergent formulation and the effective removal of pathogenic bacteria and fungi on the surface in the long term. As a result, it was observed that the probiotic B.subtilis, which can be used in the microbial based cleaner formulation, showed higher growth and vitality at the neutral pH and exhibited high antibiotic resistance, auto-aggregation, antagonistic and antifungal ability. Based on these findings, B. subtilis can be used to prevent the development of pathogenic species.

Keywords

• B. subtilis,Probiotics,Microbial-based cleaning,Probiotic cleaning system

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