Research Article
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Year 2023, Volume: 4 Issue: 2, 49 - 53, 31.12.2023
https://doi.org/10.51539/biotech.1358300

Abstract

Project Number

1919B012202271

References

  • Blakemore LM, Boes C, Cordell R, Manson MM (2013). Curcumin-induced mitotic arrest is characterized by spindle abnormalities, defects in chromosomal congression and DNA damage. Carcinogenesis 34:351-360
  • Bozgeyik E, Bagis H, Bozgeyik I, Kocahan S (2023). The roles of long non-coding RNAs in the necroptotic signaling of colon cancer cells. Mol Biol Rep 50:5021-5028
  • Cai Z, Jitkaew S, Zhao J, Chiang H-C, Choksi S, Liu J, Ward Y, Wu L-g, Liu Z-GJNcb (2014). Plasma membrane translocation of trimerized MLKL protein is required for TNF-induced necroptosis. Nat Cell Biol 16:55-65
  • Chen CC, Sureshbabul M, Chen HW, Lin YS, Lee JY, Hong QS, Yang YC, Yu SL (2013). Curcumin Suppresses Metastasis via Sp-1, FAK Inhibition, and E-Cadherin Upregulation in Colorectal Cancer. Evidence-based complementary and alternative medicine : eCAM 2013:541695
  • Chen T, Yang C, Xi Z, Chen F, Li H (2020). Reduced Caudal Type Homeobox 2 (CDX2) Promoter Methylation Is Associated with Curcumin's Suppressive Effects on Epithelial-Mesenchymal Transition in Colorectal Cancer Cells. Medical science monitor : International Medical Journal Of Experimental And Clinical Research 26:e926443
  • Cho Y, Challa S, Moquin D, Genga R, Ray TD, Guildford M, Chan FK-MJC (2009). Phosphorylation-driven assembly of the RIP1-RIP3 complex regulates programmed necrosis and virus-induced inflammation. Cell 137:1112-1123
  • Gong Y, Fan Z, Luo G, Yang C, Huang Q, Fan K, Cheng H, Jin K, Ni Q, Yu XJMc (2019). The role of necroptosis in cancer biology and therapy. Mol Cancer 18:1-17
  • Guo LD, Chen XJ, Hu YH, Yu ZJ, Wang D, Liu JZ (2013). Curcumin inhibits proliferation and induces apoptosis of human colorectal cancer cells by activating the mitochondria apoptotic pathway. Phytother Res 27:422-430
  • Guo Y, Shu L, Zhang C, Su Z-Y, Kong A-NTJBp (2015). Curcumin inhibits anchorage-independent growth of HT29 human colon cancer cells by targeting epigenetic restoration of the tumor suppressor gene DLEC1. Biochem Pharmacol94:69-78
  • Hanahan D, Weinberg RAJc (2011). Hallmarks of cancer: the next generation. Cell 144:646-674
  • He S, Wang L, Miao L, Wang T, Du F, Zhao L, Wang XJC (2009). Receptor interacting protein kinase-3 determines cellular necrotic response to TNF-α. Cell 137:1100-1111
  • Holler N, Zaru R, Micheau O, Thome M, Attinger A, Valitutti S, Bodmer J-L, Schneider P, Seed B, Tschopp JJNi (2000). Fas triggers an alternative, caspase-8–independent cell death pathway using the kinase RIP as effector molecule. Nat Immunol 1:489-495
  • Johnstone RW, Ruefli AA, Lowe SWJC (2002). Apoptosis: a link between cancer genetics and chemotherapy. Cell 108:153-164
  • Lee YJ, Park KS, Lee SH (2021). Curcumin Targets Both Apoptosis and Necroptosis in Acidity-Tolerant Prostate Carcinoma Cells. Biomed Res Int 2021:8859181
  • Liu B, Cui LS, Zhou B, Zhang LL, Liu ZH, Zhang L (2019). Monocarbonyl curcumin analog A2 potently inhibits angiogenesis by inducing ROS-dependent endothelial cell death. Acta Pharmacol Sin 40:1412-1423
  • Marley AR, Nan HJIjome, genetics (2016). Epidemiology of colorectal cancer. Int J Mol Epidemiology Genet 7:105
  • Mosieniak G, Adamowicz M, Alster O, Jaskowiak H, Szczepankiewicz AA, Wilczynski GM, Ciechomska IA, Sikora EJMoa, development (2012). Curcumin induces permanent growth arrest of human colon cancer cells: link between senescence and autophagy. Mech. Ageing Dev 133:444-455
  • Rana C, Piplani H, Vaish V, Nehru B, Sanyal SN (2015). Downregulation of PI3-K/Akt/PTEN pathway and activation of mitochondrial intrinsic apoptosis by Diclofenac and Curcumin in colon cancer. Mol Cell Biochem 402:225-241
  • Selvam C, Prabu SL, Jordan BC, Purushothaman Y, Umamaheswari A, Hosseini Zare MS, Thilagavathi R (2019). Molecular mechanisms of curcumin and its analogs in colon cancer prevention and treatment. Life Sci 239:117032
  • Sun L, Wang H, Wang Z, He S, Chen S, Liao D, Wang L, Yan J, Liu W, Lei XJC (2012). Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell 148:213-227
  • Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bray F (2021). Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians 71:209-249
  • Verma S, Singh SJVw (2008). Current and Future Status of Herbal Medicines 1:347
  • Watson JL, Hill R, Yaffe PB, Greenshields A, Walsh M, Lee PW, Giacomantonio CA, Hoskin DW (2010). Curcumin causes superoxide anion production and p53-independent apoptosis in human colon cancer cells. Cancer Lett 297:1-8

The effect of curcumin on the necroptosis signaling pathway in colon cancer cells

Year 2023, Volume: 4 Issue: 2, 49 - 53, 31.12.2023
https://doi.org/10.51539/biotech.1358300

Abstract

Colon cancer is the one of the most common types of cancer in humans. A sedentary lifestyle, increasing obesity and the consumption of food additives favor the development and occurrence of colon cancer. It is emphasized that curcumin, a yellow compound isolated from the turmeric plant, is important in preventing cancer. Studies have shown that curcumin has an anticancer effect by driving cancer cells into apoptosis, but studies showing its effect on necroptosis are inconclusive. Necroptosis is a form of programmed cell death mediated by RIP proteins and has been shown to play an important role in cancer. This study aims to determine the effect of curcumin on the necroptosis signaling pathway. For this purpose, HT-29 and HCT-116 colon cancer cells were cultured and exposed to different concentrations of curcumin and MTT experiments were performed to determine the effect on cell viability. The expression levels of RIPK1, RIPK3, and MLKL genes, which are markers of necroptosis, were analyzed by real-time PCR. It was found that the expression level of RIPK1, RIPK3, and MLKL genes significantly increased after exposure of HT -29 cells to 50 µM curcumin. Moreover, the expression of RIPK1 and MLKL genes increased in HCT-116 cells after curcumin administration. Consequently, the current data clearly suggest that curcumin is a prominent driver of necroptotic signaling-mediated colon cancer cell death.

Supporting Institution

This work was funded by The Scientific & Technological Research Council of Turkey (TUBITAK) (Grant No: 1919B012202271).

Project Number

1919B012202271

References

  • Blakemore LM, Boes C, Cordell R, Manson MM (2013). Curcumin-induced mitotic arrest is characterized by spindle abnormalities, defects in chromosomal congression and DNA damage. Carcinogenesis 34:351-360
  • Bozgeyik E, Bagis H, Bozgeyik I, Kocahan S (2023). The roles of long non-coding RNAs in the necroptotic signaling of colon cancer cells. Mol Biol Rep 50:5021-5028
  • Cai Z, Jitkaew S, Zhao J, Chiang H-C, Choksi S, Liu J, Ward Y, Wu L-g, Liu Z-GJNcb (2014). Plasma membrane translocation of trimerized MLKL protein is required for TNF-induced necroptosis. Nat Cell Biol 16:55-65
  • Chen CC, Sureshbabul M, Chen HW, Lin YS, Lee JY, Hong QS, Yang YC, Yu SL (2013). Curcumin Suppresses Metastasis via Sp-1, FAK Inhibition, and E-Cadherin Upregulation in Colorectal Cancer. Evidence-based complementary and alternative medicine : eCAM 2013:541695
  • Chen T, Yang C, Xi Z, Chen F, Li H (2020). Reduced Caudal Type Homeobox 2 (CDX2) Promoter Methylation Is Associated with Curcumin's Suppressive Effects on Epithelial-Mesenchymal Transition in Colorectal Cancer Cells. Medical science monitor : International Medical Journal Of Experimental And Clinical Research 26:e926443
  • Cho Y, Challa S, Moquin D, Genga R, Ray TD, Guildford M, Chan FK-MJC (2009). Phosphorylation-driven assembly of the RIP1-RIP3 complex regulates programmed necrosis and virus-induced inflammation. Cell 137:1112-1123
  • Gong Y, Fan Z, Luo G, Yang C, Huang Q, Fan K, Cheng H, Jin K, Ni Q, Yu XJMc (2019). The role of necroptosis in cancer biology and therapy. Mol Cancer 18:1-17
  • Guo LD, Chen XJ, Hu YH, Yu ZJ, Wang D, Liu JZ (2013). Curcumin inhibits proliferation and induces apoptosis of human colorectal cancer cells by activating the mitochondria apoptotic pathway. Phytother Res 27:422-430
  • Guo Y, Shu L, Zhang C, Su Z-Y, Kong A-NTJBp (2015). Curcumin inhibits anchorage-independent growth of HT29 human colon cancer cells by targeting epigenetic restoration of the tumor suppressor gene DLEC1. Biochem Pharmacol94:69-78
  • Hanahan D, Weinberg RAJc (2011). Hallmarks of cancer: the next generation. Cell 144:646-674
  • He S, Wang L, Miao L, Wang T, Du F, Zhao L, Wang XJC (2009). Receptor interacting protein kinase-3 determines cellular necrotic response to TNF-α. Cell 137:1100-1111
  • Holler N, Zaru R, Micheau O, Thome M, Attinger A, Valitutti S, Bodmer J-L, Schneider P, Seed B, Tschopp JJNi (2000). Fas triggers an alternative, caspase-8–independent cell death pathway using the kinase RIP as effector molecule. Nat Immunol 1:489-495
  • Johnstone RW, Ruefli AA, Lowe SWJC (2002). Apoptosis: a link between cancer genetics and chemotherapy. Cell 108:153-164
  • Lee YJ, Park KS, Lee SH (2021). Curcumin Targets Both Apoptosis and Necroptosis in Acidity-Tolerant Prostate Carcinoma Cells. Biomed Res Int 2021:8859181
  • Liu B, Cui LS, Zhou B, Zhang LL, Liu ZH, Zhang L (2019). Monocarbonyl curcumin analog A2 potently inhibits angiogenesis by inducing ROS-dependent endothelial cell death. Acta Pharmacol Sin 40:1412-1423
  • Marley AR, Nan HJIjome, genetics (2016). Epidemiology of colorectal cancer. Int J Mol Epidemiology Genet 7:105
  • Mosieniak G, Adamowicz M, Alster O, Jaskowiak H, Szczepankiewicz AA, Wilczynski GM, Ciechomska IA, Sikora EJMoa, development (2012). Curcumin induces permanent growth arrest of human colon cancer cells: link between senescence and autophagy. Mech. Ageing Dev 133:444-455
  • Rana C, Piplani H, Vaish V, Nehru B, Sanyal SN (2015). Downregulation of PI3-K/Akt/PTEN pathway and activation of mitochondrial intrinsic apoptosis by Diclofenac and Curcumin in colon cancer. Mol Cell Biochem 402:225-241
  • Selvam C, Prabu SL, Jordan BC, Purushothaman Y, Umamaheswari A, Hosseini Zare MS, Thilagavathi R (2019). Molecular mechanisms of curcumin and its analogs in colon cancer prevention and treatment. Life Sci 239:117032
  • Sun L, Wang H, Wang Z, He S, Chen S, Liao D, Wang L, Yan J, Liu W, Lei XJC (2012). Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell 148:213-227
  • Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bray F (2021). Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians 71:209-249
  • Verma S, Singh SJVw (2008). Current and Future Status of Herbal Medicines 1:347
  • Watson JL, Hill R, Yaffe PB, Greenshields A, Walsh M, Lee PW, Giacomantonio CA, Hoskin DW (2010). Curcumin causes superoxide anion production and p53-independent apoptosis in human colon cancer cells. Cancer Lett 297:1-8
There are 23 citations in total.

Details

Primary Language English
Subjects Systems Biology, Gene Expression
Journal Section Research Articles
Authors

Şevval Has 0009-0001-8904-3693

Alp Can Tuncer 0009-0000-2412-0945

İbrahim Bozgeyik 0000-0003-1483-2580

Haydar Bağış 0000-0002-1140-8058

Esra Bozgeyik

Project Number 1919B012202271
Early Pub Date December 25, 2023
Publication Date December 31, 2023
Acceptance Date October 26, 2023
Published in Issue Year 2023 Volume: 4 Issue: 2

Cite

APA Has, Ş., Tuncer, A. C., Bozgeyik, İ., Bağış, H., et al. (2023). The effect of curcumin on the necroptosis signaling pathway in colon cancer cells. Bulletin of Biotechnology, 4(2), 49-53. https://doi.org/10.51539/biotech.1358300
AMA Has Ş, Tuncer AC, Bozgeyik İ, Bağış H, Bozgeyik E. The effect of curcumin on the necroptosis signaling pathway in colon cancer cells. Bull. Biotechnol. December 2023;4(2):49-53. doi:10.51539/biotech.1358300
Chicago Has, Şevval, Alp Can Tuncer, İbrahim Bozgeyik, Haydar Bağış, and Esra Bozgeyik. “The Effect of Curcumin on the Necroptosis Signaling Pathway in Colon Cancer Cells”. Bulletin of Biotechnology 4, no. 2 (December 2023): 49-53. https://doi.org/10.51539/biotech.1358300.
EndNote Has Ş, Tuncer AC, Bozgeyik İ, Bağış H, Bozgeyik E (December 1, 2023) The effect of curcumin on the necroptosis signaling pathway in colon cancer cells. Bulletin of Biotechnology 4 2 49–53.
IEEE Ş. Has, A. C. Tuncer, İ. Bozgeyik, H. Bağış, and E. Bozgeyik, “The effect of curcumin on the necroptosis signaling pathway in colon cancer cells”, Bull. Biotechnol., vol. 4, no. 2, pp. 49–53, 2023, doi: 10.51539/biotech.1358300.
ISNAD Has, Şevval et al. “The Effect of Curcumin on the Necroptosis Signaling Pathway in Colon Cancer Cells”. Bulletin of Biotechnology 4/2 (December 2023), 49-53. https://doi.org/10.51539/biotech.1358300.
JAMA Has Ş, Tuncer AC, Bozgeyik İ, Bağış H, Bozgeyik E. The effect of curcumin on the necroptosis signaling pathway in colon cancer cells. Bull. Biotechnol. 2023;4:49–53.
MLA Has, Şevval et al. “The Effect of Curcumin on the Necroptosis Signaling Pathway in Colon Cancer Cells”. Bulletin of Biotechnology, vol. 4, no. 2, 2023, pp. 49-53, doi:10.51539/biotech.1358300.
Vancouver Has Ş, Tuncer AC, Bozgeyik İ, Bağış H, Bozgeyik E. The effect of curcumin on the necroptosis signaling pathway in colon cancer cells. Bull. Biotechnol. 2023;4(2):49-53.