Starches as solidifiers for medicinal plant micropropagations and biomass accumulations

Year 2024, Volume: 5 Issue: 1, 1 - 6, 30.06.2024

Pınar Nartop , Melis Aylin Fındıkoğlu , Meltem Taştekin

https://doi.org/10.51539/biotech.1393136

Abstract

In plant tissue and cell culture studies, media compositions are one of the most important factors affecting the micropropagation procedure's efficiency. Micropropagation studies can be conducted for commercial productions of medicinal plants, and low-cost options always have significance in large-scale productions. Some media component substitutes have been studied to reduce production costs. Agar, the media solidifier, is one of the most expensive components of media compositions. In this study, corn and wheat starches were used as media solidifiers at 80 and 100 g/L concentrations, and their effects on plant growth (shoot elongations, shoot, node, and root numbers) and biomass accumulations (shoot and root fresh and dry weights) in Lavandula officinalis and Digitalis purpurea node cultures were reported. The results showed that starch type and their concentrations significantly affected plant growth. Maximum multiple shoot number was recorded in medium supplemented with 80 g/L starch and was 61.3% higher than the control. Biomass accumulations were not statistically significant; however, higher biomass accumulations were detected in starch-added media than in control. Consequently, corn and wheat starches can be used at these concentrations as a substitute for agar to induce multiple shoot formations in L. officinalis and D. purpurea node cultures.

Keywords

Lavandula officinalis, Digitalis purpurea, Starch, Micropropagation, Biomass accumulation

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References

• Alnamer R, Alaoui K, Bouidida EH, Benjouad A, Cherrah Y (2012) Sedative and hypnotic activities of the methanolic and aqueous extracts of Lavandula officinalis from Morocco. Adv Pharmacol Sci 5. http://doi.org/10.1155/2012/270824.
• Bourgaud F, Gravot A, Milesi S, Gontier E (2001) Production of Plant Secondary Metabolites: A Historical Perspective. Plant Sci 161:839-851. https://doi.org/10.1016/S0168-9452(01)00490-3
• Daud N, Taha RM, Mohd NN, Alimon H (2011) Potential of alternative gelling agents in media for the in vitro micro-propagation of Celosia sp. Int J Bot 7(2):183-188. https://doi.org/10.3923/ijb.2011.183.188
• Deb CR, Pongener A (2010) Search of alternative substratum for agar in plant tissue culture, Curr Sci 98(1):99-102.
• Ebile PA, Opata J, Hegele S (2022) Evaluating suitable low-cost agar substitutes, clarity, stability, and toxicity for resource-poor countries' tissue culture media. In Vitro Cell Dev Biol – Plant 58:989-1001. https://doi.org/10.1007/s11627-022-10285-6.
• Gour VS, Kant T (2011) Efficacy of low cost gelling agents and carbon source alternatives during in vitro rooting of Balanites aegyptiaca and Phyllanthus emblica microshoots. Tree Forest Sci Biotechnol 5(1):58-60.
• Jain R, Babbar SB (2006) Xanthan gum: an economical substitute for agar in plant tissue culture media. Plant Cell Rep 25:81–84. https://doi.org/10.1007/s00299-005-0039-8
• Karim MZ, Amin MN, Azad MAK, Begum F, Rahman MM, Ahmad S, Alam R (2003) In vitro shoot multiplication of Chrysanthemum morifolium as affected by sucrose, agar and pH. Biotechnol 2, 115-120. https://doi.org/10.3923/biotech.2003.115.120
• Lloyd GB, McCown BH (1980) Commercial-feasible micropropagation of mountain laurel- Kalmia latifolia by use of shoot- tip culture. Proc Int Plant Prop Soc 30:421–427.
• Mohamed MAH, Alsadon AA, Al-Mohaidib MS (2009) Corn and potato starch as an alternative for Solanum tuberosum micropropagation. Afr J Biotechnol 8:9199-9203. https://doi.org/10.5897/AJB09.638
• Moraes RM, Cerdeira AL, Lourenco MV (2021) Using micropropagation to develop medicinal plants into crops. Molecules 26(6):1752.
• Moraes-Cerdeira RM, Krans JV, McChesney JD, Pereira AMS, Franca SC (1995) Cotton fiber as a substitute for agar support in tissue culture. HortScience 30(5):1082-1083. https://doi.org/10.21273/HORTSCI.30.5.1082
• Nartop P (2018) Engineering of Biomass Accumulation and Secondary Metabolite Production in Plant Cell and Tissue Cultures, Plant Metabolites and Regulation Under Environmental Stress, (eds.) Parvaiz Ahmad, Mohammad Abass Ahanger, Vijay Pratap Singh, Chapter 9, Elsevier, pp. 169-194, ISBN: 978-0-12-812689-9. https://doi.org/10.1016/B978-0-12-812689-9.00009-1
• Özkaynak E, Yüksel F, Erüst N, Şimşek T (2016) Effect of using gelling agent guar gum and different sugar sources on potato micropropagation. Turk J Agr Nat Sci 3(4):249-254.
• Rabiei Z, Rafieian-Kopaei M, Makhtari S, Alibabaei Z, Shahrani M (2014) The effect of pretreatment with different doses of Lavandula officinalis ethanolic extract on memory, learning and nociception. Biomed Aging Pathol 4(1):71-76. https://doi.org/10.1016/j.biomag.2013.10.006
• Raisi A, Davoodi F, Afshar-Ghahremani S, Taheri S, Sharifi S, Adel M (2020) Evaluation of the anesthetic and tranquilizing effects of clove powder (Syzygium aromaticum) and lavender oil (Lavandula officinalis) in convict cichlid fish (Cichlasoma nigrofasciata). Iran J Vet Surg 15(1):1-7. https://doi.org/10.30500/IVSA.2019.193913.1191
• Roca-Perez L, Boluda R, Gavidia I, Perez-Bermudez P (2004) Seasonal cardenolide production and Dop5βr gene expression in natural populations of Digitalis obscura. Phytochem 65:1869-1878. https://doi.org/10.1016/j.phytochem.2004.05.004
• Tyagi RK, Agrawal A, Mahalakshmi C, Hussain Z, Tyagi H (2007) Low-cost media for in vitro conservation of turmeric (Curcuma longa L.) and genetic stability assessment using RAPD markers. In Vitro Cell Dev Biol– Plant 43:51-58.