Investigation of antiproliferative and apoptotic effects of Rosmarinus officinalis essential oil obtained by hydrodistillation on neuroblastoma cells

Year 2024, Volume: 5 Issue: 2, 51 - 55, 31.12.2024

Pınar Yumrutaş , Mustafa Pehlivan , Murat Korkmaz , Demet Kahraman , Önder Yumrutaş

https://doi.org/10.51539/biotech.1582575

Abstract

This study was designed to determine the potential antiproliferative and apoptosis inducing activities of Rosmarinus officinalis essential oil (RE) on neuroblastoma cancer cells. For this purpose, different concentrations of RE were applied to SH-SY5Y cells for 24 hours and cell viability was determined by MTT. In addition, the percentage of early, late and non-apoptotic cells was determined by AnnexinV/propodium iodide staining to determine the induction of apoptosis. In addition, the composition of RE was determined by GC-MS. In MTT assay, it was determined that the viability of SH-SY5Y cells decreased dose-dependently as a result of the application of different concentrations of RE. Moreover, 200 mg/ml RE treatment increased the percentage of cells in the late apoptotic phase. The main compounds of RE were determined as (1R)-2,6,6-Trimethylbicyclo[3.1.1]hept-2-ene, Bicyclo[3.1.1]hept-3-en-2-one, 4,6,6-trimethyl, 1,8-Cineole, Camphor by GC-MS. In conclusion, RE is thought to be an important source of anti-proliferative and apoptosis inducing activity on neuroblastoma cells.

Keywords

Neuroblastoma, Rosmarinus officinalis, Apoptosis

References

• Bajalan I, Rouzbahani R, Pirbalouti AG, Maggi F (2017) Antioxidant and antibacterial activities of the essential oils obtained from seven Iranian populations of Rosmarinus officinalis. Ind Crops Prod 107: 305-311.
• Brewer MS (2011) Natural antioxidants: sources, compounds, mechanisms of action, and potential applications. Compr Rev Food Sci Food Saf 10:221–47.
• Dhifi W, Bellili S, Jazi S, Bahloul N, Mnif W (2016) Essential oils’ chemical characterization and investigation of some biological activities: A critical review. Medicines 3(4): 25.
• Dolghi A, Coricovac D, Dinu S, Pinzaru I, Dehelean CA, Grosu C, Chioran D, Merghes PE, Sarau CA (2022) Chemical and Antimicrobial Characterization of Mentha piperita L. and Rosmarinus officinalis L. Essential Oils and In Vitro Potential Cytotoxic Effect in Human Colorectal Carcinoma Cells. Molecules (Basel, Switzerland) 27(18):6106.
• Fulda S, Debatin K M (2003) Apoptosis pathways in neuroblastoma therapy. Cancer Lett 197(1-2): 131-135.
• González-Trujano ME, Peña EI, Martínez AL, Moreno J, Guevara-Fefer P, Déciga-Campos M López-Muñoz FJ (2007) Evaluation of the antinociceptive effect of Rosmarinus officinalis L. using three different experimental models in rodents. J Ethnopharmacol 111(3): 476–482.
• Hussain AI, Anwar F, Chatha SAS, Jabbar A, Mahboob S, Nigam PS (2010) Rosmarinus officinalis essential oil: antiproliferative, antioxidant and antibacterial activities. Braz J Microbiol 41: 1070-1078.
• Jardak M, Elloumi-Mseddi J, Aifa S, Mnif S (2017) Chemical composition, anti-biofilm activity and potential cytotoxic effect on cancer cells of Rosmarinus officinalis L. essential oil from Tunisia. Lipids Health Dis 16:1-10.
• Kamihara J, Diller LR, Foulkes WD, Michaeli O, Nakano Y, Pajtler KW, Perrino M, Scollon SR, Stewart DR, Voss S, Weksberg R, Hansford JR, Brodeur GM (2024) Neuroblastoma Predisposition and Surveillance-An Update from the 2023 AACR Childhood Cancer Predisposition Workshop. Clin Cancer Res 30(15):3137–3143.
• Letai A (2017) Apoptosis and cancer. Annu Rev Cancer Biol 1(1): 275-294.
• Nieto G (2017) Biological activities of three essential oils of the Lamiaceae family. Medicines 4(3): 63.
• Özcan MM, Chalchat JC (2008) Chemical composition and antifungal activity of rosemary (Rosmarinus officinalis L.) oil from Turkey. Int J Food Sci Nutr 59(7-8): 691-698.
• Pérez-Fons L, Garzón MT, Micol V (2010) Relationship between the antioxidant capacity and effect of rosemary (Rosmarinus officinalis L.) polyphenols on membrane phospholipid order. J Agric Food Chem 58(1): 161–171. https://doi.org/10.1021/jf9026487.
• Qadir MI, Ahmed B, Noreen S (2024) Advances in the Management of Neuroblastoma. Crit Rev Eukaryot Gene Expr 34(2):1–13.
• Rašković A, Milanović I, Pavlović N, Ćebović T, Vukmirović S, Mikov M (2014) Antioxidant activity of rosemary (Rosmarinus officinalis L.) essential oil and its hepatoprotective potential. BMC Complement Altern Med 14:225.
• Santos PASR, Avanço GB, Nerilo SB, Marcelino RIA, Janeiro V, Valadares MC, Machinski M (2016) Assessment of cytotoxic activity of rosemary (Rosmarinus officinalis L.), turmeric (Curcuma longa L.), and ginger (Zingiber officinale R.) essential oils in cervical cancer cells (HeLa). Sci World J 2016(1): 9273078.
• Wang W, Li N, Luo M, Zu Y, Efferth T (2012) Antibacterial activity and anticancer activity of Rosmarinus officinalis L. essential oil compared to that of its main components. Molecules 17(3): 2704-2713.