Serological and molecular detection of Leek yellow stripe virus infecting onion (Allium cepa L.) and leek (Allium ampeloprasum L.) in Ankara, Turkey

Year 2020, Volume: 60 Issue: 1, 49 - 56, 31.03.2020

Adyatma Santosa Filiz Ertunç

https://doi.org/10.16955/bitkorb.622150

Cited By: 3

Abstract

Onion (Allium cepa L.) has a high economic value in Turkey and it is widely used as main ingredient in Turkish menus. During 2018, 25% of 1.930.695 tons onion production in Turkey was produced in Ankara province. Recently, virus-like symptoms had been observed on onion fields but so far there was no study on onion viral diseases in Ankara province. Leek yellow stripe virus (LYSV) mainly infects leek (Allium ampeloprasum L.) but also infects and potentially causes yield reduction on onion. In August 2018, 45 onion samples show typical viral infection symptoms such as leaf stripes, leaf malformation, yellowing, and dwarfing and six leek samples showing severe yellow leaf stripe were collected from fields in Ankara province. 6 onion and 2 leek samples were positive to LYSV infection in DAS-ELISA. Total nucleic acid was then extracted from samples to be used in RT-PCR using a pair of specific primer to amplify a fragment of LYSV CP region. 6 onion and 2 leek samples were also positive in the RT-PCR by forming specific bands of 1020 bp on agarose gel. The remaining samples did not produce any band on agarose gel, thus were evaluated as negative for LYSV infection. Phylogenetic analysis showed that LYSV Ankara isolates were most similar to isolates from Serbia, Argentina and Germany. This study confirmed LYSV infection in onion and leek for the first time in Ankara province, Turkey.

Keywords

onion, leek, LYSV, Ankara

Supporting Institution

Bilimsel Araştırma Projeleri Koordinatörlüğü, Ankara Üniversitesi

Project Number

18H0447001

Thanks

Authors are grateful to Scientific Research Projects Coordinator of Ankara University for the funding of this research (Project No. 18H0447001). This research was part of a Doctoral disertation at Plant Protection Department, Ankara University. The abstract was published in the 1. International Molecular Plant Protection Congress (10-13 April 2019, Adana, Turkey).

Ankara’da soğan (Allium cepa L.) ve pırasayı (Allium ampeloprasum L.) enfekte eden Pırasa sarı çizgi virüsü’nün (Leek yellow stripe potyvirus) serolojik ve moleküler olarak tanılanması

Abstract

Soğan (Allium cepa L.) Türkiye’de yüksek derecede ekonomik öneme sahip olup, Türk menülerinde yaygın olarak kullanılmaktadır. 2018 yılında 1.930.695 tonluk Türkiye üretiminin %25’i Ankara’da yapılmıştır. Son yıllarda soğan tarlalarında virus benzeri belirtiler gözlenmiş ancak Ankara bölgesinde soğan virus hastalıkları üzerine bir çalışma yapılmamıştır. Leek yellow stripe virus (LYSV) esas olarak pırasayı (Allium ampeloprasum L.) enfekte etmekle birlikte soğanı da hastalandırmakta ve verim düşüklüğüne de neden olmaktadır. 2018 yılı ağustos ayında, tipik virüs belirtisi, örneğin yaprakta şeritler, yaprak deformasyonu, sararma ve cüceleşme belirtileri gösteren 45 soğan örneği ve yapraklarda şiddetli sarı çizgi belirtisi tasıyan 6 pırasa örneği tarladan toplanmıştır. 45 soğan örneğinin 6 tanesi, 6 pırasa örneğinin 2 tanesi DAS-ELISA çalışmalarında LYSV’ye karsı pozitif sonuç vermiştir. Örneklerden elde edilen toplam nükleik asit RT-PCR’da LYSV’ye karşı geliştirilen örtü proteinine spesifik primerlerle amplifiye edilmiştir. 6 soğan ve 2 pırasa örneği RT-PCR çalışmalarında da pozitif sonuç vermiş ve agarose jelde 1020 bp büyüklüğünde bir bant oluşturmuşlardır. Diğer örnekler agarose jelde bir bant oluşturmamış ve bunlar LYSV enfeksiyonu açısından negatif olarak değerlendirilmişlerdir. Yapılan filogenetik analiz sonucunda LYSV Ankara izolatlarının Sırbistan, Arjantin ve Almanya izolatları ile benzerlik gösterdiği görülmüştür. Bu araştırma ile LYSV enfeksiyonu Ankara ilinde ilk olarak kanıtlanmış bulunmaktadır.

Keywords

soğan, pırasa, LYSV, Ankara

Project Number

18H0447001

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