Wistar Sıçanlarında (Rattus norvegicus) Monosodyum Glutamat ile İndüklenen Uterus Tümörü Üzerinde Ganoderma mbrekobenum'un Antitümör Aktivitesi

Year 2025, Volume: 3 Issue: 1, 20 - 33, 29.05.2025

Lauretta Ofodile , Ewenodere Bikomo Viola Nicholas-okpara , Ayodeji Adekunle Emmanuel Ani Uche Kanife,

https://doi.org/10.70500/bjs.1565734

Abstract

Mantar, Ganoderma türünün yaygın olarak antikanser ve antitümör olduğu bildirilmektedir, ancak rahim tümörleri hakkında yerli türler hakkında bir rapor yok gibi görünmektedir. Monosodyum glutamatın (MSG) yüksek konsantrasyonlarda rahim tümörlerini indüklediği gösterilmiştir. Wistar cinsi sıçanlarda bir Ganoderma türünün sulu ekstraktının MSG verilen antitümör sonuçları elde edildi. Wistar sıçanları, hayvanlarda uterus tümörünü indüklemek için monosodyum glutamat (MSG) ile beslendi ve Ganoderma mbrekobenum'un su özütü ile muamele edildi. Sıçanlara hematolojik, biyokimyasal, histopatolojik incelemeler yapıldı ve akut toksisite testi (LD50) yapıldı. Sonuçlar, tedavi edilen tüm gruplardaki sıçanlarda kilo alımının gözlendiğini göstermiştir. Böbrek ve uterus, ağırlığı artan grup 4 ve 6 dışındaki tüm gruplarda kilo almadı. MSG ile beslenen gruplarda kalp ve karaciğer ağırlığında anlamlı bir artış vardı, ancak G. mbrekobenum ile tedavi edilen grupların ağırlığı azaldı. Monosodyum glutamat ile tedavi edilen grup, hayvanlarda anemi ve toksisite belirtileri gösterdi. Sıçanların böbrek fonksiyonları ve lipid profilleri oldukça değişirken, karaciğer fonksiyonları önemli ölçüde (p< 0.05) değişti. Ayrıca, sıçanlarda östrojen seviyesi yükseldi. G. mbrekobenum ile tedavi edilen hayvanlar, sıçanlarda MSG'nin etkilerinden iyileşme belirtileri gösterdi. Akut toksisite testi, hayvanların ölümünü kaydetmedi ve G. mbrekobenum özütünün, hem MSG hem de G. mbrekobenum özütü ile beslendikten sonra sıçanların östrojen seviyelerini kontrole kıyasla önemli ölçüde düşürdüğü bulundu. Histolojik incelemede sadece MSG ile beslenen sıçanların uterus dokularında büyüme/kist varlığı görülürken, G. mbrekobenum ve monosodyum glutamat (800mg/kg/vücut ağırlığı) ile beslenen sıçanlarda histopatolojik değişiklik belirtisi görülmedi. Yukarıdaki sonuçlar, G. mbrekobenum'un Wistar sıçanlarında uterus tümörünü önleyebileceğini ve kontrol edebileceğini göstermektedir.

Keywords

Anahtar Kelimeler Anahtar Kelime: Tümör, Monosodyum glutamat, Ganoderma türleri, Toksisite ve Hematoloji

Ethical Statement

“Bu araştırma, Nijerya Deklarasyonu'nun yönergelerine uygun olarak yapılmış ve Yaba Teknoloji Koleji Kurumsal Etik Grubu tarafından yetkilendirilmiştir (Etik kod YCTESC 2016004SC ve 16 Şubat 2016).

Thanks

Bu araştırma çalışması sırasındaki teknik yardımı için Lagos Üniversitesi, Idi-Araba, Lagos, Nijerya'dan Bay BC Duncan'a teşekkür ederiz

ANTITUMOUR ACTIVITY OF Ganoderma mbrekobenum ON MONOSODIUM GLUTAMATE INDUCED UTERINE TUMOUR IN WISTAR RATS (Rattus norvegicus)

Abstract

Ganoderma species is widely reported to be anticancer and antitumour but there seems to be no report on indigenous species against uterine tumours. Monosodium glutamate (MSG) has been shown to induce uterine tumours in high concentrations. Antitumour effect of the water extract of a Ganoderma species on Wistar rats given MSG was conducted. Wistar rats were fed with monosodium glutamate (MSG) to induce uterine tumour in the animals and were treated with water extract of the Ganoderma mbrekobenum. Heamatological, biochemical, histopathological examinations and acute toxicity test (LD50) were done on the animals. The heart and liver in groups fed with MSG had significant increase in weight but the groups treated with G. mbrekobenum reduced in weight. The MSG-treated group showed indications of anaemia and toxicity in the animals. Renal functions and lipid profiles of the rats were fairly altered while the hepatic functions were significantly (p< 0.05) altered. Also, the amount of estrogen in the rats was elevated. Animals exposed to G. mbrekobenum showed signs of recovery from the effects of MSG in the rats. The acute toxicity test showed no fatalities among the animals and the extract of the G. mbrekobenum was notably lowered the levels of estrogen in the rats. The histological experiment revealed the presence of growths/cysts in the uterine tissues of rats fed with MSG alone, whereas the rats treated with G. mbrekobenum (800mg/kg/body weight) exhibited no histopathological alterations. These findings indicate that the G. mbrekobenum could prevent and control uterine tumour in Wistar rats.

Keywords

Antitumour , Ganoderma , Monosodium glutamate , and Wistar rats

Ethical Statement

“This research was done in line with the guidelines of the Declaration of Nigeria, and authorized by the Institutional Ethical Group of Yaba College of Technology (Ethical code YCTESC 2016004SC and 16th February 2016).

Thanks

We thank Mr B. C. Duncan, University of Lagos, Idi-Araba, Lagos, Nigeria for his technical assistance during this research work

References

• Adotey, G, Alolga, R. N, Quarcoo, A, Yerenkyi, P, Out, P, Anang, A. K., Okine, L. K. N., Gbewonyo W.S.K., Holliday, J. C., & Lombardi, V.C. (2023). Molecular Identification and Characterization of Five Ganoderma Species from the Lower Volta River Basin of Ghana Based on Nuclear Ribosomal DNA (nrDNA) Sequences. Journal of Fungi (Basel)., 10(1), 6. doi: 10.3390/jof10010006. PMID: 38276022; PMCID: PMC10817336.
• Ahmed, H. & Aslam, M. (2018). Effect of Ganoderma lucidum (Reishi) on Hematological parameters in wistar rats. International Journal of Medical Research & Health Sciences, 7(3),151-157. www.ijmrhs.com
• Airaodion, A. I., Ogbuagu, E. O, Osemwowa E. U., Ogbuagu, U., Esonu, C. E., Agunbiade, A. P., Okereke, D. & Oloruntoba, A.P. (2019). Toxicological effect of monosodium glutamate in seasonings on human health. Global Journal of Nutrition and Food Science. 1(5), 1-9 DOI: 10.33552/GJNFS.2019.01.000522
• Anne, Z., David, B., Christoph, G., Matthias, S., & Katharina, G. (2012). Prevalence, symptoms and management of uterine fibroids: an international internet-based survey of 21,746 women. Research Article; 6, 01-011. DOI: 10.1186/1472-6874-12-6
• Cook, J.G. (1971). Creatinine assay in the presence of protein. Clinica Chimica Acta. 32(3), 485–486. DOI: 10.1016/0009-8981(71)90452-9
• Deepalakshmi, K. & Mirunalini, S. (2013). Modulatory effect of Ganoderma lucidum on expression of xenobiotic enzymes, oxidant-antioxidant and hormonal status in 7, 12-dimethylbenz(a) anthracene induced mammary carcinoma in rats. Pharmacognosy Magazine, 9 (34),167-175. DOI: 10.4103/0973-1296.111286
• Doumas, B.T., Watson, W.A. & Biggs, H.G. (1971).Albumin standards and measurement of serum-albumin with bromocresol green. Clinica Chimica Acta. 31, 87- 92. DOI: 10.1016/0009-8981(71)90365-2
• Evelyn, K. A., & Malloy, H. T. (1938). Microdetermination of oxyhaemoglobin, methaemoglobin and sulphaemoglobin in a single sample of blood. Journal of Biological Chemistry, 126,655-661. DOI:10.1016/S0021-9258(18)73873-8
• Ghorbel, I., Elwej, A., Chaabene, M., Boudawara, O., Arrakchi, R., Jamoussi, K., Sellami Boudawara, T. & Zeghal N. (2017). Effects of acrylamide graded doses on metallothioneins I and II induction and DNA fragmentation: biochemical and histomorphological changes in the liver of adult rats. Toxicology and Industrial Health, 33, 611–622. DOI: 10.1177/0748233717696613
• Gornall, A. G., Badawill, C. J., & Maxima, D. (1949). Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry, 177(2),751-766. PMID: 18110453.
• Hossen, Md. S., Billah, Md. M., Prince., E. M. Tanvir M. Chowdhury, A. Z., Md. Rahman, A., Alam, F, Sudip P, Moumoni, S, Md. Ali, Y., Bhoumik, N. C., Karim, N., Gan, S. H., & Hindawi Md. I. K. (2018). Ganoderma lucidum and Auricularia polytricha Mushrooms Protect against Carbofuran-Induced Toxicity in Rats. Evidence-Based Complementary and Alternative Medicine, 13 https://doi.org/10.1155/2018/6254929
• Imafidon, K. E., & Okunrobo, L.O. (2012). Study on Biochemical Indices of Liver Function Tests of Albino Rats Supplemented with Three Sources of Vegetable Oils. Nigerian Journal of Basic and Applied Science, 19 (2), 105-110. https://www.ajol.info/index.php/njbas/article/view/81597
• Kaplan, A. (1965). Urea nitrogen and urinary ammonia. Standard Method of Clinical Chemistry, 245-256. https://doi.org/10.1016/B978-1-4831-9686-2.50030-4
• Kashimoto, N., Ishii, S., Myojin, Y., Ushijima, M., Hayama, M. and Watanabe, H. (2010). A water-soluble extract from cultured medium of Ganoderma lucidum (Reishi) mycelia attenuates the small intestinal injury induced by anticancer drugs. Oncology Letters, 1,63–68
• King, P. R. N. & King E. J. (1954). Estimation of plasma phosphatase by determination of hydrolyzed phenol with amino antipyrine. Journal of Clinical Pathology, 7, 322-326.
• Kumar, P. & Sharma, A. (2014). Gonadotropin-releasing hormone analogs: Understanding advantages and limitations. Journal of Human Reproductive Science, 7(3), 170–174. doi: 10.4103/0974-1208.142476
• Luo, N., Guan, Q., Zheng, L., Qu, X., Dai, H., & Cheng Z. (2014). Estrogen-mediated activation of fibroblasts and its effects on the fibroid cell proliferation, Translational Research, 163(3),232-41. doi: 10.1016/j.trsl.2013.11.008. Epub 2013 Nov 20. PMID: 24316382.
• Meyer, H. H. D., Rohleder M., Sreich, W. J., Gotlenboth, R., & Ochs, A. (1997). Sexual Steroid profiles and ovarian activities of the female panda Yan Yan in zoo of Berl Muench. Tieraerzu Wochen-Schr, 100, 143-147.
• Nwajei, J. C., Onuoha, S. C.. & Essien, E. B. (2015). Effects of Oral Administration of Selected Food Seasonings Consumed in Nigeria on Some Sex Hormones of Wistar Albino Rats. IOSR Journal of Biotechnology and Biochemistry, (IOSR-JBB) 1(5), 15-21.
• Nweke, E. O.1., & Akpuaka, F. C. 1. (2019). The effect of ethanolic extract of Annona muricata leaf on the basal ganglia GSC, Biological and Pharmaceutical Sciences, 6(03), 40–44.
• Ofodile, L. N., Abraham, A., Ayoade, Y., Adamu, G. L., Nwakanma, M.N. C., Ovioma, G. O Bikomo, O. E., Ikegwu, E. & Ayodeji, A. A. (2020). Effect of the aqueous extract of Ganoderma lucidum on the haematology, estradiol, cholesterol and protein levels of Wistar rats fed with monosodium glutamate. Malaysian Journal of Pharmaceutical Sciences 18(2), 47-62 doi.org/10.21315/mjps2020.18.2.4.
• Ofodile, L. N., Omoanghe S. Isikhuemhen, Felicia N. Anike, and Ayodeji A. Adekunle (2022) Domestication and cultivation studies on indigenous Ganoderma species from Nigeria. International Journal of Medicinal Mushrooms 24(6) 69-78
• Ofodile, L. N., Uma, N., Grayer, R. J., Ogundipe, O.T. & Simmonds, M. S. J (2012). Antibacterial Compounds from the Mushroom Ganoderma colossum from Nigeria Phytotherapy Research, 26 (5), 748-751. DOI: 10.1002/ptr.3598.
• Ogbonnia, S. O., Mbaka, G. O., Anyika, E. N., Emordi, J. E., & Nwakakwa, N. (2011). An Evaluation of acute and subchronic toxicities of a Nigerian polyherbal tea remedy. Pakistan Journal of Nutrition, 10 (11), 1022-1028
• Oyetayo, O.V. (2011). Medicinal uses of mushrooms in Nigeria: Towards full and sustainable exploration. African Journal of Traditional Complementary Alternative Medicine, 8, 267-274.
• Reitman, S., & Frankel, S. A. (1957). Colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology, 28(1), 56-63. doi: 10.1093/ajcp/28.1.56. PMID: 13458125.
• Samuel, T. A., Odesanmi, O. S., James, A. B., Tafida, M. & Magbagbeola, O. A. (2013). Cytotoxic and Apoptotic Potentials of Ganoderma lucidum and Curculigo pilosa on Human Cervical Adenocarcinoma Cell Line, HeLa. Journal of Biological Sciences, 13 (2), 88-93
• Sikandar, K.S., Rehman, U. K., Muhammad, I. B., Touqeer, A. R., Tasveer, Z. B., Mohammad S. & Shahana, U. K. (2013). Diuretic Activity and Cytotoxic study of various Extracts of Ganoderma lucidum. World Applied Sciences Journal, 26(7), 964-967.
• Tietz N. W. (1999). Textbook of Clinical Chemistry, Saunders.