PlaBacter: Determination of Microbial Diversity on Plastic Polymers in Topçu Pond

Year 2024, Volume: 2 Issue: 2, 47 - 55, 29.11.2024

Berkan Yeğen , Emine Tuğçe Saraç Cebeci , Şuranur Güneş , Ezgi Doğru , Sevgi Akdeniz

https://doi.org/10.70500/bjs.1563106

Abstract

Climate change is one of the biggest problems of our world, and one of the biggest measures taken to solve the climate crisis is to reduce the consumption of oil and oil derivatives, which are considered among fossil resources. However, with the globalising world and increasing population, oil companies have turned to the plastic and packaging sector in order to meet the increasing demand for plastic consumption. This situation inevitably increases the environmental damage of plastic waste. In particular, the inability to use water resources correctly and the pollution of water by wastes left in the environment adversely affect the lives of living things on earth. Within the scope of the study, it was aimed to determine the microbial diversity living on polyethylene terephthalate (PET), which is known as the raw material of tools such as plastic bottles, food containers, and fishing line, which are left as waste in the environment, especially in rivers and lake shores. In this context, Topçu Pond, which is an important water source in the field of agricultural irrigation in Yozgat province, was selected as the study area. Genomic DNA (gDNA) was isolated from biofilm layers formed on water, sediment, and PET samples collected from the study area. The bacterial communities living in the samples were identified by analysing the 16S rDNA of the gDNAs with the next-generation sequencing method. Thus, we conducted the first microbial diversity study in Topçu Pond and defined the bacterial composition on PET samples in the pond.

Keywords

Polyethylene terephthalate, 16S rDNA, Next Generation Sequencing, Microbial diversity, Lentic system

Ethical Statement

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Supporting Institution

TÜBİTAK

Project Number

1919B012223936

Thanks

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References

• E. Yeldan and E. Voyvoda, “Low Carbon Development Pathways and Priorities for Türkiye,” WWF-Turkey Research Report, Istanbul, 2015.
• H. Cüce, “Climate Change-Related Natural Disasters: Environmental Effects and Sustainable Monitoring,” IJENT, vol. 7, no. 2, pp. 113-125, 2023.
• B. Keskin and Ş. Koçoğlu, “Sustainability of the Packaging Industry and Oil Based Plastic: Analysis of the Plastic Packaging Industry's Dependence on Oil,” EJOSAT, no.35, pp. 252-258 Apr. 2022, doi: 10.31590/ejosat.1059720.
• S. Paslı, “Plastik Katkı Maddeleri ve Sucul Ortama Etkileri,” Recep Tayyip Erdogan University Journal of Science and Engineering,, vol. 3, no. 1, pp. 40-49, Jun. 2022.
• H. Selamoğlu Çağlayan and Ü. Aytan Kopuz, “Mikroplastiklerin Deniz Çevresinde Neden Olduğu Etkiler,” Voice of Nature, no: 6, pp. 44–56, Dec. 2020.
• F. Alshehrei, "Biodegradation of Synthetic and Natural Plastic by Microorganisms," J. Appl. Environ. Microbiol., no. 5, vol. 1, pp. 8-19, 2017, doi: 10.12691/jaem-5-1-2.
• A.A. Horton, C. Svendsen, R.J. Williams, D.J. Spurgeon, and E. Lahive, “Large Microplastic Particles in Sediments of Tributaries of the River Thames, UK–Abundance, Sources and Methods for Effective Quantification," Mar. Pollut. Bull., vol. 114, no. 1, pp. 218-226, Sep. 2017, doi: 10.1016/j.marpolbul.2016.09.004.
• R. Geyer, J.R. and Jambeck, K.L. “Law, Production, Use, and Fate of All Plastics Ever Made," Sci. Adv., vol. 3, no. 7, Jul. 2017, e1700782.
• X.P. Guo, Y.R. Chen, X.L. Sun, C.L. Li, L.J. Hou, M. Liu, and Y. Yang, “Plastic Properties Affect the Composition of Prokaryotic and Eukaryotic Communities and Further Regulate the ARGs in Their Surface Biofilms," Sci. Total Environ., vol. 839, 156362, Sep. 2022, doi: 10.1016/j.scitotenv.2022.156362.
• H. Tulan, and Ö. Yalçıner Ercoşkun, “Göller Yöresinde Sürdürülebilirlik ve Dirençlilik," JMTPR, vol. 2, no. 2, pp. 89-116, Dec. 2021.
• A. Elmacı, A. Teksoy, F. O. Topaç, N. Özengin, and H. S. Başkaya, “Uluabat Gölünün Mikrobiyolojik Özelliklerinin Mevsimsel Değişiminin İzlenmesi,” UUJFE, vol. 13, no. 1, pp. 93-103, Mar. 2008, doi: 10.17482/uujfe.93575.
• A.A. Elmanama, S. Afifi, and B. Bahr, “Seasonal and Spatial Variation in the Monitoring Parameters of Gaza Beach During 2002-2003,” Environ. Res., vol. 101, pp. 25-33, Sep. 2006.
• İ. Gün and F. Y. Ekinci, “Biofilms: Microbial Life On Surfaces,” GIDA, vol. 34, no. 3, pp. 165–173, Mar. 2009.
• C. De Tender, L. I. Devriese, A. Haegeman, S. Maes, J. Vangeyte, A. Cattrijsse, and T. Ruttink, “Temporal Dynamics of Bacterial and Fungal Colonization on Plastic Debris in the North Sea,” Environ. Sci. Tech., vol. 51, no. 13, pp. 7350-7360, 2017, doi: 10.1021/acs.est.7b00697.
• J. N. Huang, B. Wen, L. Miao, X. Liu, Z. J. Li, T. F. Ma, and Z. Z. Chen, “Microplastics Drive Nitrification by Enriching Functional Microorganisms in Aquaculture Pond Waters,” Chemosphere, vol. 309, no.1, 136646, Dec. 2022, doi: 10.1016/j.chemosphere.2022.136646.
• X.P. Guo, X. L. Sun, Y. R. Chen, L. Hou, M. Liu, and Y. Yang, “Antibiotic Resistance Genes in Biofilms on Plastic Wastes in an Estuarine Environment,” Sci. Total Environ., vol. 745, 140916, Nov. 2020, doi: 10.1016/j.scitotenv.2020.140916.
• L. Cai, D. Wu, J. Xia, H. Shi, and H. Kim, “Influence of Physicochemical Surface Properties on the Adhesion of Bacteria onto Four Types of Plastics,” Sci. Total Environ., vol. 671, pp. 1101-1107, Jun. 2019, doi: 10.1016/j.scitotenv.2019.03.434.
• N. Wu, Y. Zhang, Z. Zhao, J. He, W. Li, J. Li, and Z. Niu, “Colonization Characteristics of Bacterial Communities on Microplastics Compared with Ambient Environments (Water and Sediment) in Haihe Estuary,” Sci. Total Environ., vol. 708, 134876 Mar. 2020, doi: 10.1016/j.scitotenv.2019.134876.
• H. Deng, Q. Fu, D. Li, Y. Zhang, J. He, D. Feng, and C. Ge, “Microplastic-Associated Biofilm in an Intensive Mariculture Pond: Temporal Dynamics of Microbial Communities, Extracellular Polymeric Substances and Impacts on Microplastics Properties,” J. Clean. Prod., vol. 319, 128774, Oct. 2021, doi: 10.1016/j.jclepro.2021.128774.
• C. Akgöz and S. Güler, “Topçu Göleti (Yozgat) Alg Florası I: Epilitik ve Epifitik Algler,” SUFEFD, vol. 1, no. 23, pp. 7–14, Dec. 2004.
• K. Sreedharan, R. Philip, and I.S.B. Singh, “Characterization and Virulence Potential of Phenotypically Diverse Aeromonas veronii Isolates Recovered from Moribund Freshwater Ornamental Fishes of Kerala, India,” Antonie Van Leeuwenhoek, vol. 103, no. 1, pp. 53-67, Jan. 2013, doi: 10.1007/s10482-012-9786-z.
• A. Aberoum, and H. Jooyandeh, “A Review on Occurrence and Characterization of the Aeromonas species from Marine Fishes,” WJFMS, vol. 2, no. 6, pp. 519-523, 2010.
• H.C. Tekedar, S. Kumru, J. Blom, A. D. Perkins, M. J. Griffin, H. Abdelhamed, A. Karsi, and M. L. Lawrence, “Comparative Genomics of Aeromonas veronii: Identification of a Pathotype Impacting Aquaculture Globally,” PloS one, vol. 14, no. 8, e0221018, Aug. 2019, doi: 10.1371/journal.pone.0221018.
• H. J. Han, T. Taki, H. Kondo, I. Hirono, and T. Aoki, “Pathogenic Potential of a Collagenase Gene from Aeromonas veronii,” Can. J. Microbiol., vol. 54, no. 1, pp. 1-10, Jan. 2008, doi: 10.1139/w07-109.
• J. Y. Choi, Y. Kim, E. A. Ko, Y. K. Park, W. H. Jheong, G. Ko, and K. S. Ko, “Acinetobacter Species Isolates from A Range of Environments: Species Survey and Observations of Antimicrobial Resistance,” Diagn. Microbiol. Infect. Dis., vol. 74, no. 2, pp. 177–180, Oct. 2012, doi: 10.1016/j.diagmicrobio.2012.06.023.
• P. Kämpfer, “Acinetobacter,” Encyclopedia of Food Microbiology, Elsevier Ltd, 2014, pp. 11–17. doi:10.1016/b978-0-12-384730-0.00002-1.
• J. Lalucat, M. Gomila, M. Mulet, A. Zaruma, and E. García-Valdés, “Past, Present and Future of the Boundaries of the Pseudomonas Genus: Proposal of Stutzerimonas gen Nov. ,” Syst. Appl. Microbiol., vol. 45, no. 1, 126289, 2022, doi:10.1016/j.syapm.2021.126289
• L. Hunziker, D. Bönisch, U. Groenhagen, A. Bailly, S. Schulz, and L. Weisskopf, “Pseudomonas Strains Naturally Associated with Potato Plants Produce Volatiles with High Potential for Inhibition of Phytophthora infestans,” Appl. Environ. Microbiol., vol. 81, no. 3, pp. 821–830, Feb. 2015, doi:10.1128/AEM.02999-14.
• NCBI, “Nucleotide,” https://www.ncbi.nlm.nih.gov/nuccore/CP019427.1 [Access Date: 07-October-2024].
• A. A. Lopes, Y. Yoshii, S. Yamada, M. Nagakura, Y. Kinjo, Y. Mizunoe, K. and I. Okuda, “Roles of Lytic Transglycosylases in Biofilm Formation and β-lactam Resistance in Methicillin-Resistant Staphylococcus aureus,” Antimicrob. Agents Chemother., vol. 63, no. 12, e01277-19, Dec. 2019, doi:10.1128/AAC.01277-19.
• K. C. Rice, T. Patton, S. J. Yang, A. Dumoulin, M. Bischoff, and K. W. Bayles, “Transcription of the Staphylococcus aureus cid and lrg Murein Hydrolase Regulators is Affected by Sigma Factor B,” J. Bacteriol., vol. 186, no. 10, pp. 3029–3037, May. 2004, doi:10.1128/JB.186.10.3029-3037.2004.