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Year 2016, Volume: 33 Issue: 2, 0 - 0, 01.03.2016

Kevser Erol Semra Yiğitaslan Çiğdem Ünel Bilgin Kaygısız Engin Yıldırım

https://izlik.org/JA26EG46LZ

Abstract

References

  • 1. Windebank AJ, Grisold W. Chemotherapy-induced neuropathy. J Peripher Nerv Syst 2008;13:27-46. [CrossRef]
  • 2. Wolf S, Barton D, Kottschade L, Grothey A, Loprinzi C. Chemotherapy-induced peripheral neuropathy: prevention and treatment strategies. Eur J Cancer 2008;44:1507-15. [CrossRef]
  • 3. von Schlippe M, Fowler CJ, Harland SJ. Cisplatin neurotoxicity in the treatment of metastatic germ cell tumour: time course and prognosis. Br J Cancer 2001;85:823-6. [CrossRef]
  • 4. Wu F, Lin X, Okuda T, Howell SB. DNA polymerase zeta regulates cisplatin cytotoxicity, mutagenicity, and the rate of development of cisplatin resistance. Cancer Res 2004;64:8029- 35. [CrossRef]
  • 5. McDonald ES, Randon KR, Knight A, Windebank AJ. Cisplatin preferentially binds to DNA in dorsal root ganglion neurons in vitro and in vivo: a potential mechanism for neurotoxicity. Neurobiol Dis 2005;18:305-13. [CrossRef]

Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation

Year 2016, Volume: 33 Issue: 2, 0 - 0, 01.03.2016

Kevser Erol Semra Yiğitaslan Çiğdem Ünel Bilgin Kaygısız Engin Yıldırım

https://izlik.org/JA26EG46LZ

Abstract

Background: Calcium homeostasis is considered to be important in antineoplastic as well as in neurotoxic adverse effects of cisplatin.  Aims: This study aimed to investigate the role of Ca2+ in cisplatin neurotoxicity in cultured rat dorsal root ganglia (DRG) cells.  Study Design: Cell culture study. Methods: DRG cells prepared from 1-day old Sprague-Dawley rats were used to determine the role of Ca2+ in the cisplatin (10-600 μM) neurotoxicity. The cells were incubated with cisplatin plus nimodipine (1-3 μM), dizocilpine (MK-801) (1-3 μM) or thapsigargin (100-300 nM). Toxicity of cisplatinon DRG cells was determined by the MTT assay.  Results: The neurotoxicity of cisplatin was significant when used in high concentrations (100-600 μM). Nimodipine (1 μM) but not MK-801 or thapsigargin prevented the neurotoxic effects of 200 μM of cisplatin.  Conclusion: Voltage-dependent calcium channels may play a role in cisplatin neurotoxicity.

Keywords

Calcium cisplatin dorsal root ganglia primary cell culture

References

  • 1. Windebank AJ, Grisold W. Chemotherapy-induced neuropathy. J Peripher Nerv Syst 2008;13:27-46. [CrossRef]
  • 2. Wolf S, Barton D, Kottschade L, Grothey A, Loprinzi C. Chemotherapy-induced peripheral neuropathy: prevention and treatment strategies. Eur J Cancer 2008;44:1507-15. [CrossRef]
  • 3. von Schlippe M, Fowler CJ, Harland SJ. Cisplatin neurotoxicity in the treatment of metastatic germ cell tumour: time course and prognosis. Br J Cancer 2001;85:823-6. [CrossRef]
  • 4. Wu F, Lin X, Okuda T, Howell SB. DNA polymerase zeta regulates cisplatin cytotoxicity, mutagenicity, and the rate of development of cisplatin resistance. Cancer Res 2004;64:8029- 35. [CrossRef]
  • 5. McDonald ES, Randon KR, Knight A, Windebank AJ. Cisplatin preferentially binds to DNA in dorsal root ganglion neurons in vitro and in vivo: a potential mechanism for neurotoxicity. Neurobiol Dis 2005;18:305-13. [CrossRef]
There are 5 citations in total.

Details

Other ID JA57TD96UC
Authors

Kevser Erol This is me

Semra Yiğitaslan This is me

Çiğdem Ünel This is me

Bilgin Kaygısız This is me

Engin Yıldırım This is me

Publication Date March 1, 2016
IZ https://izlik.org/JA26EG46LZ
Published in Issue Year 2016 Volume: 33 Issue: 2

Cite

APA Erol, K., Yiğitaslan, S., Ünel, Ç., Kaygısız, B., & Yıldırım, E. (2016). Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation. Balkan Medical Journal, 33(2). https://izlik.org/JA26EG46LZ
AMA 1.Erol K, Yiğitaslan S, Ünel Ç, Kaygısız B, Yıldırım E. Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation. Balkan Medical Journal. 2016;33(2). https://izlik.org/JA26EG46LZ
Chicago Erol, Kevser, Semra Yiğitaslan, Çiğdem Ünel, Bilgin Kaygısız, and Engin Yıldırım. 2016. “Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation”. Balkan Medical Journal 33 (2). https://izlik.org/JA26EG46LZ.
EndNote Erol K, Yiğitaslan S, Ünel Ç, Kaygısız B, Yıldırım E (March 1, 2016) Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation. Balkan Medical Journal 33 2
IEEE [1]K. Erol, S. Yiğitaslan, Ç. Ünel, B. Kaygısız, and E. Yıldırım, “Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation”, Balkan Medical Journal, vol. 33, no. 2, Mar. 2016, [Online]. Available: https://izlik.org/JA26EG46LZ
ISNAD Erol, Kevser - Yiğitaslan, Semra - Ünel, Çiğdem - Kaygısız, Bilgin - Yıldırım, Engin. “Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation”. Balkan Medical Journal 33/2 (March 1, 2016). https://izlik.org/JA26EG46LZ.
JAMA 1.Erol K, Yiğitaslan S, Ünel Ç, Kaygısız B, Yıldırım E. Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation. Balkan Medical Journal. 2016;33. Available at https://izlik.org/JA26EG46LZ.
MLA Erol, Kevser, et al. “Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation”. Balkan Medical Journal, vol. 33, no. 2, Mar. 2016, https://izlik.org/JA26EG46LZ.
Vancouver 1.Kevser Erol, Semra Yiğitaslan, Çiğdem Ünel, Bilgin Kaygısız, Engin Yıldırım. Evaluation of Cisplatin Neurotoxicity in Cultured Rat Dorsal Root Ganglia via Cytosolic Calcium Accumulation. Balkan Medical Journal [Internet]. 2016 Mar. 1;33(2). Available from: https://izlik.org/JA26EG46LZ