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Positive Mixed lymphocyte Culture Test Result Due to HLA-DP Mismatch

Year 2011, , 224 - 226, 01.02.2011

Tülay Kılıçaslan Ayna Hilmi Tozkır Hayriye Şentürk Çiftçi Hakan Gürkan Emre Tekgündüz Çetin Algüneş Mehmet Gürtekin Mahmut Çarin

https://doi.org/10.5174/tutfd.2009.02852.1

Abstract

Graft Versus Host Disease (GVHD) remains a major cause of morbidity and mortality in allogeneic stem cell transplantation (SCT). The MLC assay has been generally accepted as a standard test for determining HLA-D region compatibility. In this study, the MLC test has been carried out on the related recipient-donor couple who were prepared for allogeneic SCT and were found to be matched for HLA-A, -B, -Cw, DR, DQ but not for -DP. The result of the MLC test was positive. We observed that HLA-DP mismatch was responsible for the increased proliferation values in MLC test. Graft Versus Host Hastalığı (GVHH) allojeneik kök hücre nakli (KHN) seyrinde halen önemli bir morbidite ve mortalite nedenidir. Mikst lenfosit kültür (MLC-Karışık Lenfosit Kültür) testi, genellikle HLA- D bölge uyumunu saptayan standart bir test olarak kabul edilir. Bu çalışmada allojeneik KHN'ne hazırlanan ve HLA-A, -B, -Cw, -DR, -DQ lokusları uyumlu ve -DP lokusu uyumsuz olan akraba hasta -verici çiftine MLC testi yapılmıştır. Test sonucu pozitif olarak saptanmış ve HLA-DP farklılığının MLC testindeki proliferasyon değerlerindeki artışlardan sorumlu olduğu gözlemlenmiştir.

Keywords

Mixed lymphocyte culture HLA-DP stem cell transplantation

References

  • Appelbaum FR. Marrow transplantation for hematological malig- nancies: A brief review current status and futureprospect. Semin Hematol 1988;25:16.
  • Mosaad YM, Kamel H. HLA-DPB1 mismatch and acute graft-ver- sus host disease in HLA-identical sibling donors. Egypt J Immu- nol 2005;12:21-8.
  • Pere S.Reinsmoen NL, Lindstrom AL, Boyce-Jacino MT, Barbosa JJ, Faras AJ, McGlave PB and Rich SS.Frequent HLA class I and DP sequence mismatches in serologically (HLA-,B,DR) and mo- lecularly (HLA-DRB1, DQA1,DQB1) HLA identical unrelated bone marrow transplant pairs. Blood 1994;83:280-7.
  • Tiercy JM. Morel C, Freidel AC, Zwahlen F, Gebuhrer L, Betuel H, et al. Selection of unrelated donors for bone marrow transplantation is improved by HLA class II genotyping with oligonucleotide hy- bridization. Proc Natl. Acad.Sci. USA. 1991;88:7121-5. [CrossRef]
  • Mickelson EM, Guthrie LA, Etzioni R, Anasetti C, Martin PJ, Han- sen JA. Role of mixed lymphocyte culture reaction in marrow do- nor selection:matching for transplants from related haploidenti- cal donors.Tissue Antigen 1994;44;83-92. [CrossRef]
  • Peakman M., Vergani D. The human Leukocyte Antigens. In: Peakman M., Vergani D. eds. Basic and clinical immunology.1.st ed. Hurchill Livingston 1997;53-66.
  • Baxter LA, Eckels DD, Ash R, Casper J. Hunter JB, Gorski J. The prdictive value of HLA-DR oligotyping for MLC responses. Trans- plantation 1992;53:1352-7.
  • Olerup O, Moller E, Persson U. HLA-DP incompatibilities in- duce signiŞ cant proliferation in primary mixed lymphocyte cul- tures in HLA-A,-B,-DR and-DQ compatible individuals: implica- tions for allogeneic bone marrow transplantation.Tissue antijen 1990;36:194-202.
  • Santamaria P. Reindsmoen NL, Lindstrom AL, Boyce-Jacino MT, Barbosa JJ, Faras AJ, McGlave PB, Rich SS. Frequent HLA class I and DP suquence mismatch es in serologically (HLA-A,-B,-DR) and molecularly (HLA-DRB1, HLA-DQA1, HLA-DQB1) HLA-identical unrelated bone marrow transplant pairs. Blood 1994;83:280-7.
  • Beatty PG, Hansen JA, Longton GM, Thomas DE, Sanders JE, Martin PJ. Marrow transplantation from HLA-matched unrelated donors for treatment of hematologic malignancies. Transplanta- tion 1991;51:443-7. [CrossRef]
  • Mosaad YM, Kamel H. HLA-DPB1 mismatch and acute graft-ver- sus host disease in HLA-identical sibling donors. Egypt J Immu- nol. 2005;12:21-8.
  • Pénzes M, Rajczy K, Gyódi E, Petrányi GG. Infl uence of HLA- DPB1 mismatches on MLR responses: the role of high resolution HLA class II typing and MLC in unrelated donor selection for BMT. Bone Marrow Transplant 1998;22:34-7. Balkan Med J 2011; 28: 224-6
  • HLA-DP Uyumsuzluğunda Lenfosit Kültür Testi

HLA-DP Uyumsuzluğuna Bağlı Mikst Lenfosit Kültür Testindeki Pozitiflik

Year 2011, , 224 - 226, 01.02.2011

Tülay Kılıçaslan Ayna Hilmi Tozkır Hayriye Şentürk Çiftçi Hakan Gürkan Emre Tekgündüz Çetin Algüneş Mehmet Gürtekin Mahmut Çarin

https://doi.org/10.5174/tutfd.2009.02852.1

Abstract

Graft Versus Host Hastalığı (GVHH) allojeneik kök hücre nakli (KHN) seyrinde halen önemli bir morbidite ve mortalite nedenidir. Mikst lenfosit kültür (MLC-Karışık Lenfosit Kültür) testi, genellikle HLA- D bölge uyumunu saptayan standart bir test olarak kabul edilir. Bu çalışmada allojeneik KHN'ne hazırlanan ve HLA-A, -B, -Cw, -DR, -DQ lokusları uyumlu ve -DP lokusu uyumsuz olan akraba hasta-verici çiftine MLC testi yapılmıştır. Test sonucu pozitif olarak saptanmış ve HLA-DP farklılığının MLC testindeki proliferasyon değerlerindeki artışlardan sorumlu olduğu gözlemlenmiştir.

Keywords

Mikst lenfosit kültürü HLA-DP kök hücre nakli

References

  • Appelbaum FR. Marrow transplantation for hematological malig- nancies: A brief review current status and futureprospect. Semin Hematol 1988;25:16.
  • Mosaad YM, Kamel H. HLA-DPB1 mismatch and acute graft-ver- sus host disease in HLA-identical sibling donors. Egypt J Immu- nol 2005;12:21-8.
  • Pere S.Reinsmoen NL, Lindstrom AL, Boyce-Jacino MT, Barbosa JJ, Faras AJ, McGlave PB and Rich SS.Frequent HLA class I and DP sequence mismatches in serologically (HLA-,B,DR) and mo- lecularly (HLA-DRB1, DQA1,DQB1) HLA identical unrelated bone marrow transplant pairs. Blood 1994;83:280-7.
  • Tiercy JM. Morel C, Freidel AC, Zwahlen F, Gebuhrer L, Betuel H, et al. Selection of unrelated donors for bone marrow transplantation is improved by HLA class II genotyping with oligonucleotide hy- bridization. Proc Natl. Acad.Sci. USA. 1991;88:7121-5. [CrossRef]
  • Mickelson EM, Guthrie LA, Etzioni R, Anasetti C, Martin PJ, Han- sen JA. Role of mixed lymphocyte culture reaction in marrow do- nor selection:matching for transplants from related haploidenti- cal donors.Tissue Antigen 1994;44;83-92. [CrossRef]
  • Peakman M., Vergani D. The human Leukocyte Antigens. In: Peakman M., Vergani D. eds. Basic and clinical immunology.1.st ed. Hurchill Livingston 1997;53-66.
  • Baxter LA, Eckels DD, Ash R, Casper J. Hunter JB, Gorski J. The prdictive value of HLA-DR oligotyping for MLC responses. Trans- plantation 1992;53:1352-7.
  • Olerup O, Moller E, Persson U. HLA-DP incompatibilities in- duce signiŞ cant proliferation in primary mixed lymphocyte cul- tures in HLA-A,-B,-DR and-DQ compatible individuals: implica- tions for allogeneic bone marrow transplantation.Tissue antijen 1990;36:194-202.
  • Santamaria P. Reindsmoen NL, Lindstrom AL, Boyce-Jacino MT, Barbosa JJ, Faras AJ, McGlave PB, Rich SS. Frequent HLA class I and DP suquence mismatch es in serologically (HLA-A,-B,-DR) and molecularly (HLA-DRB1, HLA-DQA1, HLA-DQB1) HLA-identical unrelated bone marrow transplant pairs. Blood 1994;83:280-7.
  • Beatty PG, Hansen JA, Longton GM, Thomas DE, Sanders JE, Martin PJ. Marrow transplantation from HLA-matched unrelated donors for treatment of hematologic malignancies. Transplanta- tion 1991;51:443-7. [CrossRef]
  • Mosaad YM, Kamel H. HLA-DPB1 mismatch and acute graft-ver- sus host disease in HLA-identical sibling donors. Egypt J Immu- nol. 2005;12:21-8.
  • Pénzes M, Rajczy K, Gyódi E, Petrányi GG. Infl uence of HLA- DPB1 mismatches on MLR responses: the role of high resolution HLA class II typing and MLC in unrelated donor selection for BMT. Bone Marrow Transplant 1998;22:34-7. Balkan Med J 2011; 28: 224-6
  • HLA-DP Uyumsuzluğunda Lenfosit Kültür Testi
There are 13 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Tülay Kılıçaslan Ayna This is me

Hilmi Tozkır This is me

Hayriye Şentürk Çiftçi This is me

Hakan Gürkan This is me

Emre Tekgündüz This is me

Çetin Algüneş This is me

Mehmet Gürtekin This is me

Mahmut Çarin This is me

Publication Date February 1, 2011
Published in Issue Year 2011

Cite

APA Ayna, T. K., Tozkır, H., Çiftçi, H. Ş., Gürkan, H., et al. (2011). HLA-DP Uyumsuzluğuna Bağlı Mikst Lenfosit Kültür Testindeki Pozitiflik. Balkan Medical Journal, 2011(2), 224-226. https://doi.org/10.5174/tutfd.2009.02852.1
AMA Ayna TK, Tozkır H, Çiftçi HŞ, Gürkan H, Tekgündüz E, Algüneş Ç, Gürtekin M, Çarin M. HLA-DP Uyumsuzluğuna Bağlı Mikst Lenfosit Kültür Testindeki Pozitiflik. Balkan Medical Journal. February 2011;2011(2):224-226. doi:10.5174/tutfd.2009.02852.1
Chicago Ayna, Tülay Kılıçaslan, Hilmi Tozkır, Hayriye Şentürk Çiftçi, Hakan Gürkan, Emre Tekgündüz, Çetin Algüneş, Mehmet Gürtekin, and Mahmut Çarin. “HLA-DP Uyumsuzluğuna Bağlı Mikst Lenfosit Kültür Testindeki Pozitiflik”. Balkan Medical Journal 2011, no. 2 (February 2011): 224-26. https://doi.org/10.5174/tutfd.2009.02852.1.
EndNote Ayna TK, Tozkır H, Çiftçi HŞ, Gürkan H, Tekgündüz E, Algüneş Ç, Gürtekin M, Çarin M (February 1, 2011) HLA-DP Uyumsuzluğuna Bağlı Mikst Lenfosit Kültür Testindeki Pozitiflik. Balkan Medical Journal 2011 2 224–226.
IEEE T. K. Ayna, H. Tozkır, H. Ş. Çiftçi, H. Gürkan, E. Tekgündüz, Ç. Algüneş, M. Gürtekin, and M. Çarin, “HLA-DP Uyumsuzluğuna Bağlı Mikst Lenfosit Kültür Testindeki Pozitiflik”, Balkan Medical Journal, vol. 2011, no. 2, pp. 224–226, 2011, doi: 10.5174/tutfd.2009.02852.1.
ISNAD Ayna, Tülay Kılıçaslan et al. “HLA-DP Uyumsuzluğuna Bağlı Mikst Lenfosit Kültür Testindeki Pozitiflik”. Balkan Medical Journal 2011/2 (February 2011), 224-226. https://doi.org/10.5174/tutfd.2009.02852.1.
JAMA Ayna TK, Tozkır H, Çiftçi HŞ, Gürkan H, Tekgündüz E, Algüneş Ç, Gürtekin M, Çarin M. HLA-DP Uyumsuzluğuna Bağlı Mikst Lenfosit Kültür Testindeki Pozitiflik. Balkan Medical Journal. 2011;2011:224–226.
MLA Ayna, Tülay Kılıçaslan et al. “HLA-DP Uyumsuzluğuna Bağlı Mikst Lenfosit Kültür Testindeki Pozitiflik”. Balkan Medical Journal, vol. 2011, no. 2, 2011, pp. 224-6, doi:10.5174/tutfd.2009.02852.1.
Vancouver Ayna TK, Tozkır H, Çiftçi HŞ, Gürkan H, Tekgündüz E, Algüneş Ç, Gürtekin M, Çarin M. HLA-DP Uyumsuzluğuna Bağlı Mikst Lenfosit Kültür Testindeki Pozitiflik. Balkan Medical Journal. 2011;2011(2):224-6.