Positive Mixed lymphocyte Culture Test Result Due to HLA-DP Mismatch

Abstract

Graft Versus Host Disease (GVHD) remains a major cause of morbidity and mortality in allogeneic stem cell transplantation (SCT). The MLC assay has been generally accepted as a standard test for determining HLA-D region compatibility. In this study, the MLC test has been carried out on the related recipient-donor couple who were prepared for allogeneic SCT and were found to be matched for HLA-A, -B, -Cw, DR, DQ but not for -DP. The result of the MLC test was positive. We observed that HLA-DP mismatch was responsible for the increased proliferation values in MLC test. Graft Versus Host Hastalığı (GVHH) allojeneik kök hücre nakli (KHN) seyrinde halen önemli bir morbidite ve mortalite nedenidir. Mikst lenfosit kültür (MLC-Karışık Lenfosit Kültür) testi, genellikle HLA- D bölge uyumunu saptayan standart bir test olarak kabul edilir. Bu çalışmada allojeneik KHN'ne hazırlanan ve HLA-A, -B, -Cw, -DR, -DQ lokusları uyumlu ve -DP lokusu uyumsuz olan akraba hasta -verici çiftine MLC testi yapılmıştır. Test sonucu pozitif olarak saptanmış ve HLA-DP farklılığının MLC testindeki proliferasyon değerlerindeki artışlardan sorumlu olduğu gözlemlenmiştir.

Keywords

• Mixed lymphocyte culture, HLA-DP, stem cell transplantation

HLA-DP Uyumsuzluğuna Bağlı Mikst Lenfosit Kültür Testindeki Pozitiflik

Öz

Graft Versus Host Hastalığı (GVHH) allojeneik kök hücre nakli (KHN) seyrinde halen önemli bir morbidite ve mortalite nedenidir. Mikst lenfosit kültür (MLC-Karışık Lenfosit Kültür) testi, genellikle HLA- D bölge uyumunu saptayan standart bir test olarak kabul edilir. Bu çalışmada allojeneik KHN'ne hazırlanan ve HLA-A, -B, -Cw, -DR, -DQ lokusları uyumlu ve -DP lokusu uyumsuz olan akraba hasta-verici çiftine MLC testi yapılmıştır. Test sonucu pozitif olarak saptanmış ve HLA-DP farklılığının MLC testindeki proliferasyon değerlerindeki artışlardan sorumlu olduğu gözlemlenmiştir.

Anahtar Kelimeler

• Mikst lenfosit kültürü, HLA-DP, kök hücre nakli

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13. HLA-DP Uyumsuzluğunda Lenfosit Kültür Testi