Petroselinum Crispum is Effective in Reducing Stress-Induced Gastric Oxidative Damage

Abstract

Background: Oxidative stress has been shown to play a principal role in the pathogenesis of stress-induced gastric injury. Parsley (Petroselinum crispum) contains many antioxidants such as flavanoids, carotenoids and ascorbic acid. Aims: In this study, the histopathological and biochemical results of nutrition with a parsley-rich diet in terms of eliminating stress-induced oxidative gastric injury were evaluated. Study Design: Animal experimentation. Methods: Forty male Wistar albino rats were divided into five groups: control, stress, stress + standard diet, stress + parsley-added diet and stress + lansoprazole (LPZ) groups. Subjects were exposed to 72 hours of fasting and later immobilized and exposed to the cold at +4 degrees for 8 hours to create a severe stress condition. Samples from the animals’ stomachs were arranged for microscopic and biochemical examinations. Results: Gastric mucosal injury was obvious in rats exposed to stress. The histopathologic damage score of the stress group (7.00±0.57) was higher than that of the control group (1.50±0.22) (p<0.05). Significant differences in histopathologic damage score were found between the stress and stress + parsley-added diet groups (p<0.05), the stress and stress + standard diet groups (p<0.05), and the stress and stress + LPZ groups (p<0.05). The mean tissue malondialdehyde levels of the stress + parsley-added group and the stress + LPZ group were lower than that of the stress group (p<0.05). Parsley supported the cellular antioxidant system by increasing the mean tissue glutathione level (53.31±9.50) and superoxide dismutase (15.18±1.05) and catalase (16.68±2.29) activities. Conclusion: Oral administration of parsley is effective in reducing stress-induced gastric injury by supporting the cellular antioxidant defence system.

Keywords

• Lansoprazole, gastric oxidative damages, parsley

References

1. 1. Bandyopadhyay D, Chattopadhyay A. Reactive oxygen species-induced gastric ulceration: protection by melatonin. Curr Med Chem 2006;13:1187-202.
2. 2. Jia YT, Ma B, Wei W, Xu Y, Wang Y, Tang HT, et al. Sustained activation of nuclear factor-kappaB by reactive oxygen species is involved in the pathogenesis of stress-induced gastric damage in rats. Crit Care Med 2007;35:1582-91.
3. 3. Jia YT, Wei W, Ma B, Xu Y, Liu WJ, Wang Y, et al. Activation of p38 MAPK by reactive oxygen species is essential in a rat model of stressinduced gastric mucosal injury. J Immunol 2007;179:7808-19.
4. 4. Sahin E, Gümüşlü S. Stress-dependent induction of protein oxidation, lipid peroxidation and anti-oxidants in peripheral tissues of rats: comparison of three stress models (immobilization, cold and immobilization-cold). Clin Exp Pharmacol Physiol 2007;34:425-31.
5. 5. Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J 2012;5:9-19.
6. 6. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 2006;160:1-40.
7. 7. Gebicki JM, Nauser T, Domazou A, Steinmann D, Bounds PL, Koppenol WH. Reduction of protein radicals by GSH and ascorbate: potential biological significance. Amino Acids 2010;39:1131-7.
8. 8. Seifried HE, Anderson DE, Fisher EI, Milner JA. A review of the interaction among dietary antioxidants and reactive oxygen species. J Nutr Biochem 2007;18:567-79.

9. 9. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human diseases. Int J Biochem Cell Biol 2007;39:44-84.
10. 10. Gremse DA. Lansoprazole: pharmocokinetics, pharmocodynamics and clinical uses. Expert Opin Pharmacother 2001;2:1663-70.
11. 11. Demir A, Aydın A. A new drug in the treatment of peptic ulcer disease: Lansoprazole. Turkiye Klinikleri J Gastroenterohepatol 1998;9:1-6.
12. 12. Becker JC, Grosser N, Waltke C, Schulz S, Erdmann K, Domschke W, et al. Beyond gastric acid reduction: proton pump inhibitors induce heme oxygenase-1 in gastric and endothelial cells. Biochem Biophys Res Commun 2006;345:1014-21.
13. 13. Colin-Jones DG. Safety of lansoprasole. Aliment Pharmacol Ther 1993;7(Suppl 1):56-60.
14. 14. Altunbaş M, Turel I. The lethal dose levels of volatile oil extract of Petroselinum crispum (Parsley) seeds and the investigation of antiinflammatory effect on laboratory animals. YYU Vet Fak Derg 2009;20:21- 5.
15. 15. Fejes S, Blazovics A, Lemberkovics E, Petri G, Szoke E, Kery A. Free radical scavenging and membrane protective effects of methanol extracts from Anthriscus cerefolium L. (Hoffm.) and Petroselinum crispum (Mill.) nym. ex A.W. Hill. Phytother Res 2000;14:362-5.
16. 16. Gadi D, Bnouham M, Aziz M, Ziyyat A, Legssyer A, Legrand C, et al. Parsley extract inhibits in vitro and ex vivo platelet aggregation and prolongs bleeding time in rats. J Ethnopharmacol 2009;125:170-4.
17. 17. Al-Howiriny T, Al-Sohaibani M, El-Tahir K, Rafatullah S. Prevention of experimentally-induced gastric ulcers in rats by an ethanolic extract of ‘Parsley’ Petroselinum crispum. Am J Chin Med 2003;31:699-711.
18. 18. Mahmood S, Hussain S, Malik F. Critique of medicinal conspicuousness of Parsley (Petroselinum crispum): a culinary herb of Mediterranean region. Pak J Pharm Sci 2014;27:193-202.
19. 19. Haidari F, Keshavarz SA, Mohammad Shahi M, Mahboob SA, Rashidi MR. Effects of Parsley (Petroselinum crispum) and its Flavonol Constituents, Kaempferol and Quercetin, on Serum Uric Acid Levels, Biomarkers of Oxidative Stress and Liver Xanthine Oxidoreductase Aactivity in OxonateInduced Hyperuricemic Rats. Iran J Pharm Res 2011;10:811-9.
20. 20. Tang EL, Rajarajeswaran J, Fung S, Kanthimathi MS. Petroselinum crispum has antioxidant properties, protects against DNA damage and inhibits proliferation and migration of cancer cells. J Sci Food Agric 2015;95:2763-71.
21. 21. Dorman HJ, Lantto TA, Raasmaja A, Hiltunen R. Antioxidant, pro-oxidant and cytotoxic properties of parsley. Food Funct 2011;2:328-37.
22. 22. Fejes S, Kéry A, Blázovics A, Lugasi A, Lemberkovics E, Petri G, et al. Investigation of the in vitro antioxidant effect of Petroselinum crispum (Mill.) Nym. ex A. W. Hill. Acta Pharm Hung 1998;68:150-6.
23. 23. Mota KS, Dias GE, Pinto ME, Luiz-Ferreira A, Souza-Brito AR, HirumaLima CA, et al. Flavonoids with gastroprotective activity. Molecules 2009;14:979-1012.
24. 24. Akinci A, Esrefoglu M, Cetin A, Ates B. Melatonin is more effective than ascorbic acid and β-carotene in improvement of gastric mucosal damage induced by intensive stress. Arch Med Sci 2015;11:1129-36.
25. 25. Popovic M, Kaurinovic B, Jakovljevic V, Mimica-Dukic N, Bursac M. Effect of Parsley (Petroselinum crispum (Mill.) Nym. ex A.W. Hill, Apiaceae) extracts on some biochemical parameters of oxidative stress in mice treated with CCI (4). Phytother Res 2007;21:717-23.
26. 26. Bradford MM. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.
27. 27. Luck H. Methods of enzymatic analysis. New York: Verlag Chemie, Academic Press; 1963.
28. 28. McCord JM, Fridovich, I. Superoxide dismutase. An enzymatic function for erythrocuprein (hemocuprein). J Biol Che 1969;244:6049-55.
29. 29. Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 1978;52:302-10.
30. 30. Lawrence RA, Burk RF. Glutathione peroxidase activity in seleniumdeficient rat liver. Biochem Biopys Res Commun 1976;71:952-8.