Effects of Restraint Stress and Nitric Oxide Synthase Inhibition on Learning and Strategy Preference in Young Adult Male Rats

Year 2012, , 376 - 380, 01.04.2012

Melih Dağdeviren Yusuf Hakan Doğan Lütfiye Kanıt

https://doi.org/10.5152/balkanmedj.2012.100

Abstract

Objective: The aim of this study was to investigate the effects of restraint stress and nitric oxide synthase (NOS) inhibition by NωNitro-L-Arginine (LNA) on learning and strategy preference. Material and Methods: Rats were randomly divided into four groups (Saline, Saline+Stress, LNA, LNA+Stress). Stress was applied for one hour in glass cylinders during 13 days. One hour after this stress application, water maze experiments were started. Injections (saline 1 ml/kg or 50 mg/kg LNA) were given 10 minutes before each experiment. The platform was kept visible or hidden (on the 4th, 8th, 12th days) at the same position. On the 13th day the platform was located on the opposite quadrant. Results: Saline groups exhibited significantly better performances (F(1.31)=174.038 p<0.05) at the beginning compared to the NOS inhibited groups. For initial hidden platform days; stress was determined as an impairment factor (F(1.31)=5.190 p=0.012). At the end, acquisition occurred on both visible and hidden platform days for all groups. There was no significant strategy preference difference between the groups.Development of the stress and NOS inhibition impairments were seen, particularly at different periods of the acquisition. Conclusion: NOS inhibition did not worsen restraint stress-induced learning impairments in rats. Lack of effect may be explained by the antidepressive consequences of NOS inhibition. Turkish Başlık: Kısıtlama stresi ve nitrik oksit sentaz inhibisyonunun genç erişkin erkek sıçanlarda öğrenme ve strateji tercihi üzerine etkileri Anahtar Kelimeler: Diabetes mellitus, cynodon dactylon, elektromanyetik alan, insülin, glukoz Amaç: Prospektif, randomize ve çilf kör çalışmada septorinoplasti (SRP) ve endoskopik sinus cerrahisi (ESC) ameliyatlarından sonra levobupivakain ve tramadol infiltrasyonunun postoperatif analjezik etkilerinin araştırılması amaçlandı. Gereç ve Yöntemler: Çalışmaya elektif olarak ESC ve SRP operasyonları gerirecek ASA I-III sınıfı altmış erişkin hasta alındı. Anestezi indüksiyonu intravenöz propofol 2-2.5 mg/kg-1, roküronyum bromid 0.6 mg/kg-1, and fentanil 1 μg/kg-1 ile gerçekleştirildi. İdame %2 sevofluran ile N2O/O2 karışımı (FiO2: 35%) ile sağlandı. Operasyondan 10 dakika önce cerrahi sahaya tramadol 0.5 mg/kg-1(Group T: n= 20), levobupivakain %0.25(Group L: n= 20), ve lidokainin %1(Group C: n= 20) adrenaline solüsyonu 1/200000 infiltre edildi (ESC için 5 mL ve SRP için10 mL). Postoperatif dönemde hastaların tümüne hasta kontrollü analjezi cihazı ile fentanil uygulandı (bolus doz: 15 μg ve kilit aralığı:10 dak). Ağrı postoperative ilk 24 saatte 11 dereceli vizüel analog skala (VAS) ile 4 saat ara ile değerlendirildi. Postoperatif dönemde analjezik gereksinim, opioid tüketimi ve yan etkiler belirlenerek kaydedildi. Bulgular: Tramadol verilen grubun fentanil gereksinim ve tüketimi istatistiksel açıdan farklı bulundu. 24 saatlik fentanyl dozları Grup C, L ve T için sırası ile 345.2 ± 168.8,6 μg, 221.1 ± 120.6 μg; and 184.1 ± 130,3 μg (p= 0.002) olarak belirlendi. Tramadol ve kontrol grubunun fentanil gereksinimlerinde 16, 20 ve 24. saat peryotlarda (p= 0.012, p= 0.004. ve p= 0.002) farklılık saptandı. Yan etki profili benzerdi. Sonuç: Bulgularımız ESC ve SRP operasyonlarından önce preemptif tramadol infiltrasyonunun levobupivakainin etkili, pratik ve güvenli bir alternatifi olduğunu düşündürmektedir.

Keywords

Learning, NOS inhibition, restraint stress, strategy preference, water maze

Effects of Restraint Stress and Nitric Oxide Synthase Inhibition on Learning and Strategy Preference in Young Adult Male Rats

Abstract

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