In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii

Year 2013, , 19 - 22, 01.01.2013

Melek Bilgin Tuba Yıldırım Murat Hökelek

https://doi.org/10.5152/balkanmedj.2012.098

Abstract

Objective: Ivermectin and sulphadiazine were tested individually to determine their in vitro effects on Toxoplasma gondii grown in human epidermoid larynx carcinoma (Hep-2) cell culture. Study Design: In-vitro study. Material and Methods: Toxoplasma growth was quantities by an enzyme immunoassay performed directly on the fixed cultures, using a rabbit anti-T. gondii immunoglobulin G as the first antibody and a phosphatase-labeled anti-rabbit immunoglobulin G as the second antibody. For each drug, regression models were used to quantify the relationship between optical density values and antimicrobial agent concentrations in the cultures. Results: The 50% inhibitory concentrations (IC50) of ivermectin and sulphadiazine were found to be 0.2 μg/mL and 7.3 μg/mL after 48 h of exposure, respectively. None of the concentrations tested for each drugs demonstrated toxicity to Hep-2 cells after 72 h of incubation. Conclusion: These results indicate that ivermectin significantly inhibited replication of the tachyzoites of T. gondii RH strain. Turkish Başlık: İvermektin ve sulfadiazinin toxoplasma gondıı'ye etkinliklerinin in vitro değerlendirilmesi Anahtar Kelimeler: Toxoplasma gondii, ivermektin, sulfadiazin, in vitro, Hep-2 Amaç: İvermektin ile sulfadiazinin, in vitro T. gondii ile enfekte insan larenks epidermoid karsinom (Hep-2) hücrelerinde anti-toxoplasma aktiviteleri araştırılmıştır. Gereç ve Yöntemler: Antimikrobiyal ajanların anti-Toxoplasma aktiviteleri hücre kültür tabanlı ELISA ile değerlendirilmiştir. Primer antikor olarak tavşan anti-T gondii immunglobulin G sekonder antikor olarak alkalen fosfataz işaretli anti-rabbit immunglobulin G kullanılmıştır. Her ilaç için, optik dansite değerleri ile antimikrobiyal ajan konsantrasyonları arasındaki ilişki regresyon analizi uygulanarak değerlendirilmiştir. Bulgular: İvermektin ve sulfadiazinin inkübasyonun 48. saatinde, %50 inhibitör konsantrasyon (IC50) değerleri sırasıyla 0.2 μg/ml ve 7.3 μg/ml olarak hesaplanmıştır. Ayrıca her iki ilacın test edilen konsantrasyonlarının Hep-2 hücrelerine toksik olmadığı saptanmıştır. Sonuç: Bu sonuçlar; ivermektinin in vitro T.gondii RH suşu takizoit replikasyonunu anlamlı olarak inhibe ettiğini göstermiştir.

Keywords

Toxoplasma gondii, ivermectin, sulphadiazine, in vitro, Hep-2

In Vitro Effects of Ivermectin and Sulphadiazine on Toxoplasma gondii

Abstract

References

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