Protective Effects of Alpha-Lipoic Acid on Oleic Acid-Induced Acute Lung Injury in Rats

Abstract

Background: Oxidative stress is believed to be an important factor in the pathogenesis of acute lung injury (ALI). Aims: The aim of this study was to investigate the possible protective role of alpha-lipoic acid (α-LA) on oleic acid (OA)-induced ALI in rats. Study Design: Animal experiment. Methods: A total of thirty-five rats were divided into five groups in the study. Group 1 served as a control group. Rats in Group 2 (α-LA) were administered α-LA intraperitoneally at a dose of 100 mg/kg body weight (BW). Rats in Group 3 (OA) were administered OA intravenously at a dose of 100 mg/kg BW. In Group 4 (pre-OA-α-LA), α-LA was given 15 minutes prior to OA infusion, and in Group 5 (post-OA-α-LA), α-LA was given two hours after OA infusion. Four hours after the OA infusion, rats were decapitated. Blood samples were collected to measure serum levels of malondialdehyde (MDA) and glutathione (GSH), and the levels of activity for superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px). Lung tissue samples were taken for histopathological examination. Results: Exposure to OA resulted in increases in serum MDA levels (p<0.001), as well as histopathological lesions in lung tissue, and decreases in CAT (p<0.05), GSH-Px (p<0.05) activities and GSH (p<0.05) levels. On the other hand, MDA levels were decreased significantly (p<0.001), while CAT (p<0.05), GSH-Px (p<0.01) activities and GSH (p<0.05) levels were increased significantly in the pre-OA-α-LA group compared with the OA group. Conclusion: α-LA was found to lessen oxidative stress and to have positive effects on antioxidants in cases of OA-induced ALI. In conclusion, α-LA appears to have protective effects against ALI and potential for the prevention of ALI. Turkish Başlık: Ratlarda oleik asit ile oluşturulan akut akciğer hasarında alfa-lipoik asidin koruyucu etkileri Anahtar Kelimeler: Akut akciğer hasarı, oleik asit, α-lipoik asit, oksidatif stres. Arkaplan: Oksidatif stresin akut akciğer hasarı (ALI) patogenezinde önemli bir faktör olduğu bilinmektedir. Amaç: Bu çalışmanın amacı, ratlarda oleik asit (OA) ile oluşturulan akut akciğer hasarında alfa-lipoik asidin (α-LA) koruyucu etkilerinin araştırılmasıdır. Çalışma Tasarımı: Deneysel çalışma. Yöntemler: Çalışmada toplam otuz beş rat beş gruba ayrıldı. Grup 1 kontrol grubu olarak ele alındı. Grup 2 (α-LA) ratlara 100 mg/kg dozda α-LA intraperitoneal (i.p) olarak uygulandı. Grup 3 (OA) ratlara 100 mg/kg dozda oleik asit intravenöz (i.v.) olarak uygulandı. Grup 4'te (pre-OA-α-LA) OA infüzyonundan 15 dakika önce; Grup 5'te (post-OA-α-LA) ise OA infüzyonundan 2 saat sonra α-LA verildi. OA infüzyonundan dört saat sonra ratlar dekapite edildi. Serum malondialdehit (MDA) ve glutatyon (GSH) düzeyleri ile süperoksit dismutaz (SOD), katalaz (CAT) ve glutatyon peroksidaz (GSH-Px) aktiviteleri ölçümü için kan örnekleri ile histopatolojik inceleme için akciğer doku örnekleri alındı. Bulgular: OA infüzyonunun akciğer dokusunda histopatolojik lezyonlarla birlikte, serum MDA düzeylerinde artışa (p<0.001); CAT (p<0.05) ve GSH-Px (p<0.05) aktiviteleri ile GSH (p<0.05) düzeylerinde ise azalmaya yol açtığı belirlenmiştir. Buna karşılık, OA grubu ile karşılaştırıldığında pre-OA-α-LA grubunda serum MDA düzeylerinin anlamlı şekilde azaldığı (p<0.001); CAT (p<0.05) ve GSH-Px (p<0.01) aktiviteleri ile GSH (p<0.05) düzeylerinin ise anlamlı şekilde arttığı tespit edilmiştir. Sonuç: OA ile oluşturulan akciğer hasarında α-LA'nın oksidatif stresi azalttığı ve antioksidanlar üzerinde olumlu etkilere sahip olduğu ortaya konmuştur. Sonuç olarak; α-LA'nın akciğer hasarına karşı koruyucu etkisi olduğu ve hasarın önlenmesi açısından önemli bir potansiyele sahip olduğu düşünülebilir.

Keywords

• Acute lung injury, oleic acid, α-lipoic acid, oxidative stress

Protective Effects of Alpha-Lipoic Acid on Oleic Acid-Induced Acute Lung Injury in Rats

Öz

Anahtar Kelimeler

• xx

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